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Compressive loading of the murine tibia reveals site-specific micro-scale differences in adaptation and maturation rates of bone

Bergström, I.; Kerns, J. G.; Törnqvist, A. E.; Perdikouri, C. LU ; Mathavan, N. LU ; Koskela, A.; Henriksson, H. B.; Tuukkanen, J.; Andersson, G. and Isaksson, H. LU , et al. (2016) In Osteoporosis International
Abstract

Summary: Loading increases bone mass and strength in a site-specific manner; however, possible effects of loading on bone matrix composition have not been evaluated. Site-specific structural and material properties of mouse bone were analyzed on the macro- and micro/molecular scale in the presence and absence of axial loading. The response of bone to load is heterogeneous, adapting at molecular, micro-, and macro-levels. Introduction: Osteoporosis is a degenerative disease resulting in reduced bone mineral density, structure, and strength. The overall aim was to explore the hypothesis that changes in loading environment result in site-specific adaptations at molecular/micro- and macro-scale in mouse bone. Methods: Right tibiae of adult... (More)

Summary: Loading increases bone mass and strength in a site-specific manner; however, possible effects of loading on bone matrix composition have not been evaluated. Site-specific structural and material properties of mouse bone were analyzed on the macro- and micro/molecular scale in the presence and absence of axial loading. The response of bone to load is heterogeneous, adapting at molecular, micro-, and macro-levels. Introduction: Osteoporosis is a degenerative disease resulting in reduced bone mineral density, structure, and strength. The overall aim was to explore the hypothesis that changes in loading environment result in site-specific adaptations at molecular/micro- and macro-scale in mouse bone. Methods: Right tibiae of adult mice were subjected to well-defined cyclic axial loading for 2 weeks; left tibiae were used as physiologically loaded controls. The bones were analyzed with μCT (structure), reference point indentation (material properties), Raman spectroscopy (chemical), and small-angle X-ray scattering (mineral crystallization and structure). Results: The cranial and caudal sites of tibiae are structurally and biochemically different within control bones. In response to loading, cranial and caudal sites increase in cortical thickness with reduced mineralization (−14 and −3%, p < 0.01, respectively) and crystallinity (−1.4 and −0.3%, p < 0.05, respectively). Along the length of the loaded bones, collagen content becomes more heterogeneous on the caudal site and the mineral/collagen increases distally at both sites. Conclusion: Bone structure and composition are heterogeneous, finely tuned, adaptive, and site-specifically responsive at the micro-scale to maintain optimal function. Manipulation of this heterogeneity may affect bone strength, relative to specific applied loads.

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publication status
epub
subject
keywords
Loading, Raman spectroscopy, RPI, SAXS
in
Osteoporosis International
pages
11 pages
publisher
Springer
external identifiers
  • scopus:85002152411
  • wos:000394258000040
ISSN
0937-941X
DOI
10.1007/s00198-016-3846-6
language
English
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yes
id
6d65f635-5442-48c1-9bb3-ea4364fca368
date added to LUP
2016-12-28 09:35:04
date last changed
2017-09-18 11:33:27
@article{6d65f635-5442-48c1-9bb3-ea4364fca368,
  abstract     = {<p>Summary: Loading increases bone mass and strength in a site-specific manner; however, possible effects of loading on bone matrix composition have not been evaluated. Site-specific structural and material properties of mouse bone were analyzed on the macro- and micro/molecular scale in the presence and absence of axial loading. The response of bone to load is heterogeneous, adapting at molecular, micro-, and macro-levels. Introduction: Osteoporosis is a degenerative disease resulting in reduced bone mineral density, structure, and strength. The overall aim was to explore the hypothesis that changes in loading environment result in site-specific adaptations at molecular/micro- and macro-scale in mouse bone. Methods: Right tibiae of adult mice were subjected to well-defined cyclic axial loading for 2 weeks; left tibiae were used as physiologically loaded controls. The bones were analyzed with μCT (structure), reference point indentation (material properties), Raman spectroscopy (chemical), and small-angle X-ray scattering (mineral crystallization and structure). Results: The cranial and caudal sites of tibiae are structurally and biochemically different within control bones. In response to loading, cranial and caudal sites increase in cortical thickness with reduced mineralization (−14 and −3%, p &lt; 0.01, respectively) and crystallinity (−1.4 and −0.3%, p &lt; 0.05, respectively). Along the length of the loaded bones, collagen content becomes more heterogeneous on the caudal site and the mineral/collagen increases distally at both sites. Conclusion: Bone structure and composition are heterogeneous, finely tuned, adaptive, and site-specifically responsive at the micro-scale to maintain optimal function. Manipulation of this heterogeneity may affect bone strength, relative to specific applied loads.</p>},
  author       = {Bergström, I. and Kerns, J. G. and Törnqvist, A. E. and Perdikouri, C. and Mathavan, N. and Koskela, A. and Henriksson, H. B. and Tuukkanen, J. and Andersson, G. and Isaksson, H. and Goodship, A. E. and Windahl, S. H.},
  issn         = {0937-941X},
  keyword      = {Loading,Raman spectroscopy,RPI,SAXS},
  language     = {eng},
  month        = {12},
  pages        = {11},
  publisher    = {Springer},
  series       = {Osteoporosis International},
  title        = {Compressive loading of the murine tibia reveals site-specific micro-scale differences in adaptation and maturation rates of bone},
  url          = {http://dx.doi.org/10.1007/s00198-016-3846-6},
  year         = {2016},
}