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Antigenic and biochemical characteristics of Mobiluncus mulieris and Mobiluncus curtisii

Skarin, A LU (1986) In Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology 94(3). p.33-127
Abstract

Twenty-four strains of Mobiluncus mulieris and 27 strains of Mobiluncus curtisii were tested with respect to 6 different biochemical characteristics: arginin-decarboxylase activity, beta-galactosidase activity, synergistic hemolysis with Staphylococcus aureus, hydrolysis of hippurate, migration through soft agar and reduction of nitrate. Antigens of the same strains, prepared by ultrasonication, were subjected to sodium dodecyl sulphate-polyacrylamide gel electrophoresis followed by immunoblotting using polyclonal rabbit antisera against two of the M. mulieris strains and five of the M. curtisii strains. Two different strongly reacting protein antigens could be detected in the M. mulieris strains. These strains could be separated into... (More)

Twenty-four strains of Mobiluncus mulieris and 27 strains of Mobiluncus curtisii were tested with respect to 6 different biochemical characteristics: arginin-decarboxylase activity, beta-galactosidase activity, synergistic hemolysis with Staphylococcus aureus, hydrolysis of hippurate, migration through soft agar and reduction of nitrate. Antigens of the same strains, prepared by ultrasonication, were subjected to sodium dodecyl sulphate-polyacrylamide gel electrophoresis followed by immunoblotting using polyclonal rabbit antisera against two of the M. mulieris strains and five of the M. curtisii strains. Two different strongly reacting protein antigens could be detected in the M. mulieris strains. These strains could be separated into three groups based on the possession of either of the two antigens or both. In the M. curtisii strains, 10 strongly reacting protein antigens could be detected. Four strains possessed only one of these antigens, one did not possess any, while the remaining strains possessed different sets of 9 of them. Within each species common protein antigens were detected. No antigens were found which were shared by both species. The biochemical characteristics studied could not differentiate between the antigenic groups in any of the species. None of the antigenic subgroups of M. curtisii found in the present study was identical with any of the two subspecies (curtisii and holmesii) which have been proposed.

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organization
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Contribution to journal
publication status
published
subject
keywords
Antigens, Bacterial/analysis, Bacteria, Anaerobic/classification, Carboxy-Lyases/metabolism, Electrophoresis, Polyacrylamide Gel, Female, Hemolysis, Hippurates/metabolism, Humans, Immunosorbent Techniques, Nitrates/metabolism, Vagina/microbiology, beta-Galactosidase/metabolism
in
Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology
volume
94
issue
3
pages
33 - 127
publisher
John Wiley & Sons Inc.
external identifiers
  • pmid:3090855
  • scopus:0022728315
ISSN
0108-0180
DOI
10.1111/j.1699-0463.1986.tb03031.x
language
English
LU publication?
yes
id
6d8212ea-0c33-48da-8667-89e64999bc20
date added to LUP
2022-07-12 09:23:17
date last changed
2024-01-02 21:58:52
@article{6d8212ea-0c33-48da-8667-89e64999bc20,
  abstract     = {{<p>Twenty-four strains of Mobiluncus mulieris and 27 strains of Mobiluncus curtisii were tested with respect to 6 different biochemical characteristics: arginin-decarboxylase activity, beta-galactosidase activity, synergistic hemolysis with Staphylococcus aureus, hydrolysis of hippurate, migration through soft agar and reduction of nitrate. Antigens of the same strains, prepared by ultrasonication, were subjected to sodium dodecyl sulphate-polyacrylamide gel electrophoresis followed by immunoblotting using polyclonal rabbit antisera against two of the M. mulieris strains and five of the M. curtisii strains. Two different strongly reacting protein antigens could be detected in the M. mulieris strains. These strains could be separated into three groups based on the possession of either of the two antigens or both. In the M. curtisii strains, 10 strongly reacting protein antigens could be detected. Four strains possessed only one of these antigens, one did not possess any, while the remaining strains possessed different sets of 9 of them. Within each species common protein antigens were detected. No antigens were found which were shared by both species. The biochemical characteristics studied could not differentiate between the antigenic groups in any of the species. None of the antigenic subgroups of M. curtisii found in the present study was identical with any of the two subspecies (curtisii and holmesii) which have been proposed.</p>}},
  author       = {{Skarin, A}},
  issn         = {{0108-0180}},
  keywords     = {{Antigens, Bacterial/analysis; Bacteria, Anaerobic/classification; Carboxy-Lyases/metabolism; Electrophoresis, Polyacrylamide Gel; Female; Hemolysis; Hippurates/metabolism; Humans; Immunosorbent Techniques; Nitrates/metabolism; Vagina/microbiology; beta-Galactosidase/metabolism}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{33--127}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology}},
  title        = {{Antigenic and biochemical characteristics of Mobiluncus mulieris and Mobiluncus curtisii}},
  url          = {{http://dx.doi.org/10.1111/j.1699-0463.1986.tb03031.x}},
  doi          = {{10.1111/j.1699-0463.1986.tb03031.x}},
  volume       = {{94}},
  year         = {{1986}},
}