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Identification of Iridoid Glucoside Transporters in Catharanthus roseus

Larsen, Lars Bo; Fuller, Victoria L; Pollier, Jacob; Van Moerkercke, Alex LU ; Schweizer, Fabian; Payne, Richard J.; Colinas, Maite; O'Connor, Sarah E; Goossens, Alain and Halkier, Barbara A (2017) In Plant and Cell Physiology 58(9). p.1507-1518
Abstract

Monoterpenoid indole alkaloids (MIAs) are plant defense compounds and high-value pharmaceuticals. Biosynthesis of the universal MIA precursor, secologanin, is organized between internal phloem-associated parenchyma (IPAP) and epidermis cells. Transporters for intercellular transport of proposed mobile pathway intermediates have remained elusive. Screening of an Arabidopsis thaliana transporter library expressed in Xenopus oocytes identified AtNPF2.9 as a putative iridoid glucoside importer. Eight orthologs were identified in Catharanthus roseus, of which three, CrNPF2.4, CrNPF2.5 and CrNPF2.6, were capable of transporting the iridoid glucosides 7-deoxyloganic acid, loganic acid, loganin and secologanin into oocytes. Based on enzyme... (More)

Monoterpenoid indole alkaloids (MIAs) are plant defense compounds and high-value pharmaceuticals. Biosynthesis of the universal MIA precursor, secologanin, is organized between internal phloem-associated parenchyma (IPAP) and epidermis cells. Transporters for intercellular transport of proposed mobile pathway intermediates have remained elusive. Screening of an Arabidopsis thaliana transporter library expressed in Xenopus oocytes identified AtNPF2.9 as a putative iridoid glucoside importer. Eight orthologs were identified in Catharanthus roseus, of which three, CrNPF2.4, CrNPF2.5 and CrNPF2.6, were capable of transporting the iridoid glucosides 7-deoxyloganic acid, loganic acid, loganin and secologanin into oocytes. Based on enzyme expression data and transporter specificity, we propose that several enzymes of the biosynthetic pathway are present in both IPAP and epidermis cells, and that the three transporters are responsible for transporting not only loganic acid, as previously proposed, but multiple intermediates. Identification of the iridoid glucoside-transporting CrNPFs is an important step toward understanding the complex orchestration of the seco-iridioid pathway.

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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Plant and Cell Physiology
volume
58
issue
9
pages
12 pages
publisher
Oxford University Press
ISSN
1471-9053
DOI
10.1093/pcp/pcx097
language
English
LU publication?
no
id
6d8bde2a-50ca-41af-861f-ee68946ab1c3
date added to LUP
2017-11-06 11:27:34
date last changed
2018-05-08 03:00:36
@article{6d8bde2a-50ca-41af-861f-ee68946ab1c3,
  abstract     = {<p>Monoterpenoid indole alkaloids (MIAs) are plant defense compounds and high-value pharmaceuticals. Biosynthesis of the universal MIA precursor, secologanin, is organized between internal phloem-associated parenchyma (IPAP) and epidermis cells. Transporters for intercellular transport of proposed mobile pathway intermediates have remained elusive. Screening of an Arabidopsis thaliana transporter library expressed in Xenopus oocytes identified AtNPF2.9 as a putative iridoid glucoside importer. Eight orthologs were identified in Catharanthus roseus, of which three, CrNPF2.4, CrNPF2.5 and CrNPF2.6, were capable of transporting the iridoid glucosides 7-deoxyloganic acid, loganic acid, loganin and secologanin into oocytes. Based on enzyme expression data and transporter specificity, we propose that several enzymes of the biosynthetic pathway are present in both IPAP and epidermis cells, and that the three transporters are responsible for transporting not only loganic acid, as previously proposed, but multiple intermediates. Identification of the iridoid glucoside-transporting CrNPFs is an important step toward understanding the complex orchestration of the seco-iridioid pathway.</p>},
  author       = {Larsen, Lars Bo and Fuller, Victoria L and Pollier, Jacob and Van Moerkercke, Alex and Schweizer, Fabian and Payne, Richard J. and Colinas, Maite and O'Connor, Sarah E and Goossens, Alain and Halkier, Barbara A},
  issn         = {1471-9053},
  language     = {eng},
  month        = {09},
  number       = {9},
  pages        = {1507--1518},
  publisher    = {Oxford University Press},
  series       = {Plant and Cell Physiology},
  title        = {Identification of Iridoid Glucoside Transporters in Catharanthus roseus},
  url          = {http://dx.doi.org/10.1093/pcp/pcx097},
  volume       = {58},
  year         = {2017},
}