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Differences in lipid composition between free-living and initially adhered cells of a Gram-negative bacterium

Valeur, Anders ; Tunlid, Anders LU and Odham, Göran (1988) In Archives of Microbiology 149(6). p.521-526
Abstract

The phospholipid fatty acid composition and poly-β-hydroxybutyrate (PHB) content of initially adhered and free-living cells of a Pseudomonas sp. isolated from the rape plant Brassica napus were examined with gas chromatography (GC). Five different adhesion experiments were made including variations in surface charge (hydrophilic and lipophilic), temperature, media composition and time of adhesion. Lipids and poly-β-hydroxybutyrate (PHB) were extracted with a chloroform-methanol-water mixture, hydrolyzed and esterified with pentafluorobenzyl bromide. Analysis was performed with capillary gas chromatography and flame ionization detection. A pronounced difference in both the ratio saturated/unsaturated fatty acids and in PHB content... (More)

The phospholipid fatty acid composition and poly-β-hydroxybutyrate (PHB) content of initially adhered and free-living cells of a Pseudomonas sp. isolated from the rape plant Brassica napus were examined with gas chromatography (GC). Five different adhesion experiments were made including variations in surface charge (hydrophilic and lipophilic), temperature, media composition and time of adhesion. Lipids and poly-β-hydroxybutyrate (PHB) were extracted with a chloroform-methanol-water mixture, hydrolyzed and esterified with pentafluorobenzyl bromide. Analysis was performed with capillary gas chromatography and flame ionization detection. A pronounced difference in both the ratio saturated/unsaturated fatty acids and in PHB content between free-living and adhered bacteria were found. The free-living bacteria has a significantly smaller ratio of saturated/unsaturated C16 and C18 fatty acids and also a smaller ratio of total C18/total C16 fatty acids. Bacteria adhered to the lipophilic surface had a higher ratio of saturated to unsaturated C16 fatty acids than at the hydrophilic surface. There were no major differences between the treatments regarding the amount of bacteria adhered to the surface or their lipid composition.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Adhesion, Bacterial membrane, Capillary gas chromatography, Fatty acid ratios, Pseudomonas, Subpopulation
in
Archives of Microbiology
volume
149
issue
6
pages
521 - 526
publisher
Springer
external identifiers
  • scopus:0000940312
ISSN
0302-8933
DOI
10.1007/BF00446755
language
English
LU publication?
yes
id
6d987c4e-34f4-468c-8d8b-a254fda7973d
date added to LUP
2019-10-23 17:27:31
date last changed
2020-01-13 02:28:43
@article{6d987c4e-34f4-468c-8d8b-a254fda7973d,
  abstract     = {<p>The phospholipid fatty acid composition and poly-β-hydroxybutyrate (PHB) content of initially adhered and free-living cells of a Pseudomonas sp. isolated from the rape plant Brassica napus were examined with gas chromatography (GC). Five different adhesion experiments were made including variations in surface charge (hydrophilic and lipophilic), temperature, media composition and time of adhesion. Lipids and poly-β-hydroxybutyrate (PHB) were extracted with a chloroform-methanol-water mixture, hydrolyzed and esterified with pentafluorobenzyl bromide. Analysis was performed with capillary gas chromatography and flame ionization detection. A pronounced difference in both the ratio saturated/unsaturated fatty acids and in PHB content between free-living and adhered bacteria were found. The free-living bacteria has a significantly smaller ratio of saturated/unsaturated C<sub>16</sub> and C<sub>18</sub> fatty acids and also a smaller ratio of total C<sub>18</sub>/total C<sub>16</sub> fatty acids. Bacteria adhered to the lipophilic surface had a higher ratio of saturated to unsaturated C<sub>16</sub> fatty acids than at the hydrophilic surface. There were no major differences between the treatments regarding the amount of bacteria adhered to the surface or their lipid composition.</p>},
  author       = {Valeur, Anders and Tunlid, Anders and Odham, Göran},
  issn         = {0302-8933},
  language     = {eng},
  month        = {04},
  number       = {6},
  pages        = {521--526},
  publisher    = {Springer},
  series       = {Archives of Microbiology},
  title        = {Differences in lipid composition between free-living and initially adhered cells of a Gram-negative bacterium},
  url          = {http://dx.doi.org/10.1007/BF00446755},
  doi          = {10.1007/BF00446755},
  volume       = {149},
  year         = {1988},
}