Secretion of plasminogen activator and lysosomal enzymes from mouse skeletal muscle: effect of denervation
(1995) In Journal of Cellular Physiology 164(3). p.555-561- Abstract
- Levels of hydrolytic enzymes increase in skeletal muscle after denervation and their activities in the extracellular matrix appear to be important for interaction between muscle and nerve. Using enzymatic assays for beta-glucuronidase, beta-galactosidase, and plasminogen activator, we show that secretion of these enzymes from mouse skeletal muscle increases after denervation and that drugs interfering with the secretory pathway or the reuptake of enzymes modulate this release. Thus, brefeldin A inhibited secretion of plasminogen activator activity and mannan increased secreted amounts of beta-glucuronidase, but not of beta-galactosidase, in denervated muscle. In innervated muscle, brefeldin A decreased secreted activity of plasminogen... (More)
- Levels of hydrolytic enzymes increase in skeletal muscle after denervation and their activities in the extracellular matrix appear to be important for interaction between muscle and nerve. Using enzymatic assays for beta-glucuronidase, beta-galactosidase, and plasminogen activator, we show that secretion of these enzymes from mouse skeletal muscle increases after denervation and that drugs interfering with the secretory pathway or the reuptake of enzymes modulate this release. Thus, brefeldin A inhibited secretion of plasminogen activator activity and mannan increased secreted amounts of beta-glucuronidase, but not of beta-galactosidase, in denervated muscle. In innervated muscle, brefeldin A decreased secreted activity of plasminogen activator, but mannan had no effect on secretion of either beta-glucuronidase or beta-galactosidase. Furthermore, secretion of plasminogen activator was temperature dependent. These observations, together with previous studies, suggest that secretion of hydrolytic enzymes from adult skeletal muscle may be of physiological significance in nerve-muscle communication. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1109588
- author
- Vult von Steyern, Fredrik LU and Josefsson, Jan-Owe LU
- organization
- publishing date
- 1995
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Cellular Physiology
- volume
- 164
- issue
- 3
- pages
- 555 - 561
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:7650063
- scopus:0029151019
- ISSN
- 1097-4652
- DOI
- 10.1002/jcp.1041640313
- language
- English
- LU publication?
- yes
- id
- 6df61f98-90bc-4979-8b56-e01bb1bd7495 (old id 1109588)
- date added to LUP
- 2016-04-01 12:00:32
- date last changed
- 2021-01-03 07:07:20
@article{6df61f98-90bc-4979-8b56-e01bb1bd7495, abstract = {{Levels of hydrolytic enzymes increase in skeletal muscle after denervation and their activities in the extracellular matrix appear to be important for interaction between muscle and nerve. Using enzymatic assays for beta-glucuronidase, beta-galactosidase, and plasminogen activator, we show that secretion of these enzymes from mouse skeletal muscle increases after denervation and that drugs interfering with the secretory pathway or the reuptake of enzymes modulate this release. Thus, brefeldin A inhibited secretion of plasminogen activator activity and mannan increased secreted amounts of beta-glucuronidase, but not of beta-galactosidase, in denervated muscle. In innervated muscle, brefeldin A decreased secreted activity of plasminogen activator, but mannan had no effect on secretion of either beta-glucuronidase or beta-galactosidase. Furthermore, secretion of plasminogen activator was temperature dependent. These observations, together with previous studies, suggest that secretion of hydrolytic enzymes from adult skeletal muscle may be of physiological significance in nerve-muscle communication.}}, author = {{Vult von Steyern, Fredrik and Josefsson, Jan-Owe}}, issn = {{1097-4652}}, language = {{eng}}, number = {{3}}, pages = {{555--561}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Journal of Cellular Physiology}}, title = {{Secretion of plasminogen activator and lysosomal enzymes from mouse skeletal muscle: effect of denervation}}, url = {{http://dx.doi.org/10.1002/jcp.1041640313}}, doi = {{10.1002/jcp.1041640313}}, volume = {{164}}, year = {{1995}}, }