Advanced

Modulation of miRNA expression in aged rat hippocampus by buttermilk and krill oil

Crespo, M. Carmen ; Tomé-Carneiro, Joao ; Gómez-Coronado, Diego ; Burgos-Ramos, Emma ; Garciá-Serrano, Alba LU ; Martín-Hernández, Roberto ; Baliyan, Shishir ; Fontecha, Javier ; Venero, César and Dávalos, Alberto , et al. (2018) In Scientific Reports 8(1).
Abstract

The increasing incidence of age-induced cognitive decline justifies the search for complementary ways of prevention or delay. We studied the effects of concentrates of phospholipids, sphingolipids, and/or 3-n fatty acids on the expression of genes or miRNAs related to synaptic activity and/or neurodegeneration, in the hippocampus of aged Wistar rats following a 3-month supplementation. The combination of two phospholipidic concentrates of krill oil (KOC) and buttermilk (BMFC) origin modulated the hippocampal expression of 119 miRNAs (11 were common to both BMFC and BMFC + KOC groups). miR-191a-5p and miR-29a-3p changed significantly only in the BMFC group, whereas miR-195-3p and miR-148a-5p did so only in the combined-supplemented... (More)

The increasing incidence of age-induced cognitive decline justifies the search for complementary ways of prevention or delay. We studied the effects of concentrates of phospholipids, sphingolipids, and/or 3-n fatty acids on the expression of genes or miRNAs related to synaptic activity and/or neurodegeneration, in the hippocampus of aged Wistar rats following a 3-month supplementation. The combination of two phospholipidic concentrates of krill oil (KOC) and buttermilk (BMFC) origin modulated the hippocampal expression of 119 miRNAs (11 were common to both BMFC and BMFC + KOC groups). miR-191a-5p and miR-29a-3p changed significantly only in the BMFC group, whereas miR-195-3p and miR-148a-5p did so only in the combined-supplemented group. Thirty-eight, 58, and 72 differentially expressed genes (DEG) were found in the groups supplemented with KOC, BMFC and BMFC + KOC, respectively. Interaction analysis unveiled networks of selected miRNAs with their potential target genes. DEG found in the KOC and BMFC groups were mainly involved in neuroactive processes, whereas they were associated with lysosomes and mRNA surveillance pathways in the BMFC + KOC group. We also report a significant reduction in hippocampal ceramide levels with BMFC + KOC. Our results encourage additional in-depth investigations regarding the potential beneficial effects of these compounds.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and , et al. (More)
; ; ; ; ; ; ; ; ; and (Less)
publishing date
type
Contribution to journal
publication status
published
subject
in
Scientific Reports
volume
8
issue
1
article number
3993
publisher
Nature Publishing Group
external identifiers
  • scopus:85043233251
  • pmid:29507352
ISSN
2045-2322
DOI
10.1038/s41598-018-22148-5
language
English
LU publication?
no
id
6ed9cfd6-20e1-44c9-a2cd-6bbb85799f98
date added to LUP
2020-02-05 15:15:31
date last changed
2021-02-17 05:25:38
@article{6ed9cfd6-20e1-44c9-a2cd-6bbb85799f98,
  abstract     = {<p>The increasing incidence of age-induced cognitive decline justifies the search for complementary ways of prevention or delay. We studied the effects of concentrates of phospholipids, sphingolipids, and/or 3-n fatty acids on the expression of genes or miRNAs related to synaptic activity and/or neurodegeneration, in the hippocampus of aged Wistar rats following a 3-month supplementation. The combination of two phospholipidic concentrates of krill oil (KOC) and buttermilk (BMFC) origin modulated the hippocampal expression of 119 miRNAs (11 were common to both BMFC and BMFC + KOC groups). miR-191a-5p and miR-29a-3p changed significantly only in the BMFC group, whereas miR-195-3p and miR-148a-5p did so only in the combined-supplemented group. Thirty-eight, 58, and 72 differentially expressed genes (DEG) were found in the groups supplemented with KOC, BMFC and BMFC + KOC, respectively. Interaction analysis unveiled networks of selected miRNAs with their potential target genes. DEG found in the KOC and BMFC groups were mainly involved in neuroactive processes, whereas they were associated with lysosomes and mRNA surveillance pathways in the BMFC + KOC group. We also report a significant reduction in hippocampal ceramide levels with BMFC + KOC. Our results encourage additional in-depth investigations regarding the potential beneficial effects of these compounds.</p>},
  author       = {Crespo, M. Carmen and Tomé-Carneiro, Joao and Gómez-Coronado, Diego and Burgos-Ramos, Emma and Garciá-Serrano, Alba and Martín-Hernández, Roberto and Baliyan, Shishir and Fontecha, Javier and Venero, César and Dávalos, Alberto and Visioli, Francesco},
  issn         = {2045-2322},
  language     = {eng},
  month        = {12},
  number       = {1},
  publisher    = {Nature Publishing Group},
  series       = {Scientific Reports},
  title        = {Modulation of miRNA expression in aged rat hippocampus by buttermilk and krill oil},
  url          = {http://dx.doi.org/10.1038/s41598-018-22148-5},
  doi          = {10.1038/s41598-018-22148-5},
  volume       = {8},
  year         = {2018},
}