Tryptophan regulates the expression of IGFBP1 in bovine endometrial epithelial cells in vitro via the TDO2-AHR pathway
Wang, Peng-Chao ; Liu, Ze-Kun ; Li, Jia-Rong ; Zhao, Zi-Hui ; Chang, Qian-Wen ; Guo, Xiao-Min ; Jin, Lin ; Hu, Yong-Ting and Yang, Zhenshan LU
(2024)
In BMC Veterinary Research
20.
p.1-9
- Abstract
BACKGROUND: This study aimed to identify the roles of L-tryptophan (Trp) and its rate-limiting enzymes on the receptivity of bovine endometrial epithelial cells. Real-time PCR was conducted to analyze the differential expression of genes between different groups of bovine endometrial epithelial cells. Western blot was performed to detect Cyclooxygenase-2 (COX2) expression after treatment with Trp or kynurenine (the main metabolites of Trp). The kynurenine assay was used to examine if Trp or prostaglandin E2 (PGE2) can increase the production of kynurenine in the bovine endometrial epithelial cells.
RESULTS: Trp significantly stimulates insulin growth factor binding protein 1 (IGFBP1) expression, a common endometrial marker of... (More)
BACKGROUND: This study aimed to identify the roles of L-tryptophan (Trp) and its rate-limiting enzymes on the receptivity of bovine endometrial epithelial cells. Real-time PCR was conducted to analyze the differential expression of genes between different groups of bovine endometrial epithelial cells. Western blot was performed to detect Cyclooxygenase-2 (COX2) expression after treatment with Trp or kynurenine (the main metabolites of Trp). The kynurenine assay was used to examine if Trp or prostaglandin E2 (PGE2) can increase the production of kynurenine in the bovine endometrial epithelial cells.
RESULTS: Trp significantly stimulates insulin growth factor binding protein 1 (IGFBP1) expression, a common endometrial marker of conceptus elongation and uterus receptivity for ruminants. When bovine endometrial epithelial cells are treated with Trp, tryptophan hydroxylase-1 remains unchanged, but tryptophan 2,3-dioxygenase 2 (TDO2) is significantly increased, suggesting tryptophan is mainly metabolized through the kynurenine pathway. Kynurenine significantly stimulates IGFBP1 expression. Furthermore, Trp and kynurenine significantly increase the expression of aryl hydrocarbon receptor (AHR). CH223191, an AHR inhibitor, abrogates the induction of Trp and kynurenine on IGFBP1. PGE2 significantly induces the expression of TDO2, AHR, and IGFBP1.
CONCLUSIONS: The regulation between Trp / kynurenine and PGE2 may be crucial for the receptivity of the bovine uterus.
(Less)
- author
- Wang, Peng-Chao
; Liu, Ze-Kun
; Li, Jia-Rong
; Zhao, Zi-Hui
; Chang, Qian-Wen
; Guo, Xiao-Min
; Jin, Lin
; Hu, Yong-Ting
and Yang, Zhenshan
LU
- organization
- publishing date
- 2024-09-04
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Animals, Cattle, Female, Tryptophan/pharmacology, Endometrium/metabolism, Epithelial Cells/metabolism, Insulin-Like Growth Factor Binding Protein 1/metabolism, Receptors, Aryl Hydrocarbon/metabolism, Kynurenine/metabolism, Tryptophan Oxygenase/metabolism, Dinoprostone/metabolism, Gene Expression Regulation/drug effects, Signal Transduction/drug effects, Cyclooxygenase 2/metabolism
- in
- BMC Veterinary Research
- volume
- 20
- article number
- 390
- pages
- 1 - 9
- publisher
- BioMed Central (BMC)
- external identifiers
-
- scopus:85203007394
- pmid:39227948
- ISSN
- 1746-6148
- DOI
- 10.1186/s12917-024-04191-9
- language
- English
- LU publication?
- yes
- additional info
- © 2024. The Author(s).
- id
- 6ef14534-ed83-48c4-a4c2-85f935de69d2
- date added to LUP
- 2024-10-09 15:27:16
- date last changed
- 2025-07-04 03:30:45
@article{6ef14534-ed83-48c4-a4c2-85f935de69d2,
abstract = {{<p>BACKGROUND: This study aimed to identify the roles of L-tryptophan (Trp) and its rate-limiting enzymes on the receptivity of bovine endometrial epithelial cells. Real-time PCR was conducted to analyze the differential expression of genes between different groups of bovine endometrial epithelial cells. Western blot was performed to detect Cyclooxygenase-2 (COX2) expression after treatment with Trp or kynurenine (the main metabolites of Trp). The kynurenine assay was used to examine if Trp or prostaglandin E2 (PGE2) can increase the production of kynurenine in the bovine endometrial epithelial cells.</p><p>RESULTS: Trp significantly stimulates insulin growth factor binding protein 1 (IGFBP1) expression, a common endometrial marker of conceptus elongation and uterus receptivity for ruminants. When bovine endometrial epithelial cells are treated with Trp, tryptophan hydroxylase-1 remains unchanged, but tryptophan 2,3-dioxygenase 2 (TDO2) is significantly increased, suggesting tryptophan is mainly metabolized through the kynurenine pathway. Kynurenine significantly stimulates IGFBP1 expression. Furthermore, Trp and kynurenine significantly increase the expression of aryl hydrocarbon receptor (AHR). CH223191, an AHR inhibitor, abrogates the induction of Trp and kynurenine on IGFBP1. PGE2 significantly induces the expression of TDO2, AHR, and IGFBP1.</p><p>CONCLUSIONS: The regulation between Trp / kynurenine and PGE2 may be crucial for the receptivity of the bovine uterus.</p>}},
author = {{Wang, Peng-Chao and Liu, Ze-Kun and Li, Jia-Rong and Zhao, Zi-Hui and Chang, Qian-Wen and Guo, Xiao-Min and Jin, Lin and Hu, Yong-Ting and Yang, Zhenshan}},
issn = {{1746-6148}},
keywords = {{Animals; Cattle; Female; Tryptophan/pharmacology; Endometrium/metabolism; Epithelial Cells/metabolism; Insulin-Like Growth Factor Binding Protein 1/metabolism; Receptors, Aryl Hydrocarbon/metabolism; Kynurenine/metabolism; Tryptophan Oxygenase/metabolism; Dinoprostone/metabolism; Gene Expression Regulation/drug effects; Signal Transduction/drug effects; Cyclooxygenase 2/metabolism}},
language = {{eng}},
month = {{09}},
pages = {{1--9}},
publisher = {{BioMed Central (BMC)}},
series = {{BMC Veterinary Research}},
title = {{Tryptophan regulates the expression of IGFBP1 in bovine endometrial epithelial cells in vitro via the TDO2-AHR pathway}},
url = {{http://dx.doi.org/10.1186/s12917-024-04191-9}},
doi = {{10.1186/s12917-024-04191-9}},
volume = {{20}},
year = {{2024}},
}