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MHC expression in fragment and full-thickness allogeneic embryonic retinal transplants

Ghosh, F LU ; Larsson, Jörgen LU and Wilke, Kennerth (2000) In Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie 238(7). p.98-589
Abstract

BACKGROUND: The study was carried out to evaluate the expression of major histocompatibility complex (MHC) molecules in retinal transplants with different tissue integrity.

METHODS: Twelve adult rabbits received an allogeneic subretinal neuroretinal transplant, in the form of either fragmented embryonic cells or a complete full-thickness embryonic retina. A controlled transvitreal approach was used for both transplantation types. The grafts were examined histologically after 31 or 49 days with hematoxylin and eosin staining and immunohistochemical analysis of MHC class I and class II expression.

RESULTS: All five fragment transplants developed into rosettes. Two of them displayed MHC class I-labeled cells, and four MHC class... (More)

BACKGROUND: The study was carried out to evaluate the expression of major histocompatibility complex (MHC) molecules in retinal transplants with different tissue integrity.

METHODS: Twelve adult rabbits received an allogeneic subretinal neuroretinal transplant, in the form of either fragmented embryonic cells or a complete full-thickness embryonic retina. A controlled transvitreal approach was used for both transplantation types. The grafts were examined histologically after 31 or 49 days with hematoxylin and eosin staining and immunohistochemical analysis of MHC class I and class II expression.

RESULTS: All five fragment transplants developed into rosettes. Two of them displayed MHC class I-labeled cells, and four MHC class II-labeled cells. The cells were concentrated on the scleral side of the graft, and there was also a marked increase of labeled cells in the choroid. MHC labeling was often associated with defects in the retinal pigment epithelium. Six of the seven full-thickness grafts displayed a laminated morphology with well-developed retinal layers. The seventh consisted of rosettes. None of these grafts displayed MHC class I- or class II-labeled cells.

CONCLUSIONS: The findings suggest that host immune response against fragmented and intact neuroretinal grafts is different, indicating tissue integrity as one factor affecting graft-host immune interactions. The absence of immune response in full-thickness grafts is encouraging and important in the struggle to find therapies for retinal degenerative disease.

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keywords
Animals, Biomarkers, Fetal Tissue Transplantation, Fluorescent Antibody Technique, Indirect, Graft Rejection, Histocompatibility Antigens Class I, Histocompatibility Antigens Class II, Microscopy, Fluorescence, Pigment Epithelium of Eye, Rabbits, Retina, Transplantation, Homologous, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
in
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie
volume
238
issue
7
pages
10 pages
publisher
Springer
external identifiers
  • scopus:0033944592
ISSN
0721-832X
DOI
10.1007/s004170000138
language
English
LU publication?
yes
id
6ef8a592-f759-4630-bac4-fe463221c8fe
date added to LUP
2017-05-17 11:37:38
date last changed
2017-05-29 15:22:14
@article{6ef8a592-f759-4630-bac4-fe463221c8fe,
  abstract     = {<p>BACKGROUND: The study was carried out to evaluate the expression of major histocompatibility complex (MHC) molecules in retinal transplants with different tissue integrity.</p><p>METHODS: Twelve adult rabbits received an allogeneic subretinal neuroretinal transplant, in the form of either fragmented embryonic cells or a complete full-thickness embryonic retina. A controlled transvitreal approach was used for both transplantation types. The grafts were examined histologically after 31 or 49 days with hematoxylin and eosin staining and immunohistochemical analysis of MHC class I and class II expression.</p><p>RESULTS: All five fragment transplants developed into rosettes. Two of them displayed MHC class I-labeled cells, and four MHC class II-labeled cells. The cells were concentrated on the scleral side of the graft, and there was also a marked increase of labeled cells in the choroid. MHC labeling was often associated with defects in the retinal pigment epithelium. Six of the seven full-thickness grafts displayed a laminated morphology with well-developed retinal layers. The seventh consisted of rosettes. None of these grafts displayed MHC class I- or class II-labeled cells.</p><p>CONCLUSIONS: The findings suggest that host immune response against fragmented and intact neuroretinal grafts is different, indicating tissue integrity as one factor affecting graft-host immune interactions. The absence of immune response in full-thickness grafts is encouraging and important in the struggle to find therapies for retinal degenerative disease.</p>},
  author       = {Ghosh, F and Larsson, Jörgen and Wilke, Kennerth},
  issn         = {0721-832X},
  keyword      = {Animals,Biomarkers,Fetal Tissue Transplantation,Fluorescent Antibody Technique, Indirect,Graft Rejection,Histocompatibility Antigens Class I,Histocompatibility Antigens Class II,Microscopy, Fluorescence,Pigment Epithelium of Eye,Rabbits,Retina,Transplantation, Homologous,Comparative Study,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {7},
  pages        = {98--589},
  publisher    = {Springer},
  series       = {Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie},
  title        = {MHC expression in fragment and full-thickness allogeneic embryonic retinal transplants},
  url          = {http://dx.doi.org/10.1007/s004170000138},
  volume       = {238},
  year         = {2000},
}