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Versatile microporous polymer-based supports for serial macromolecular crystallography

Martiel, Isabelle ; Beale, John H. ; Karpik, Agnieszka ; Huang, Chia Ying ; Vera, Laura ; Olieric, Natacha ; Wranik, Maximilian ; Tsai, Ching Ju ; Mühle, Jonas and Aurelius, Oskar LU , et al. (2021) In Acta Crystallographica Section D: Structural Biology 77. p.1153-1167
Abstract

Serial data collection has emerged as a major tool for data collection at state-of-the-art light sources, such as microfocus beamlines at synchrotrons and X-ray free-electron lasers. Challenging targets, characterized by small crystal sizes, weak diffraction and stringent dose limits, benefit most from these methods. Here, the use of a thin support made of a polymer-based membrane for performing serial data collection or screening experiments is demonstrated. It is shown that these supports are suitable for a wide range of protein crystals suspended in liquids. The supports have also proved to be applicable to challenging cases such as membrane proteins growing in the sponge phase. The sample-deposition method is simple and robust, as... (More)

Serial data collection has emerged as a major tool for data collection at state-of-the-art light sources, such as microfocus beamlines at synchrotrons and X-ray free-electron lasers. Challenging targets, characterized by small crystal sizes, weak diffraction and stringent dose limits, benefit most from these methods. Here, the use of a thin support made of a polymer-based membrane for performing serial data collection or screening experiments is demonstrated. It is shown that these supports are suitable for a wide range of protein crystals suspended in liquids. The supports have also proved to be applicable to challenging cases such as membrane proteins growing in the sponge phase. The sample-deposition method is simple and robust, as well as flexible and adaptable to a variety of cases. It results in an optimally thin specimen providing low background while maintaining minute amounts of mother liquor around the crystals. The 2 × 2 mm area enables the deposition of up to several microlitres of liquid. Imaging and visualization of the crystals are straightforward on the highly transparent membrane. Thanks to their affordable fabrication, these supports have the potential to become an attractive option for serial experiments at synchrotrons and free-electron lasers.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Fixed target, Sample supports, Serial crystallography, SFX
in
Acta Crystallographica Section D: Structural Biology
volume
77
pages
15 pages
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:85114344941
  • pmid:34473086
ISSN
2059-7983
DOI
10.1107/S2059798321007324
language
English
LU publication?
yes
id
6f11c53a-32a9-447a-9a72-49aa131732c7
date added to LUP
2021-10-07 12:39:53
date last changed
2025-01-13 14:43:39
@article{6f11c53a-32a9-447a-9a72-49aa131732c7,
  abstract     = {{<p>Serial data collection has emerged as a major tool for data collection at state-of-the-art light sources, such as microfocus beamlines at synchrotrons and X-ray free-electron lasers. Challenging targets, characterized by small crystal sizes, weak diffraction and stringent dose limits, benefit most from these methods. Here, the use of a thin support made of a polymer-based membrane for performing serial data collection or screening experiments is demonstrated. It is shown that these supports are suitable for a wide range of protein crystals suspended in liquids. The supports have also proved to be applicable to challenging cases such as membrane proteins growing in the sponge phase. The sample-deposition method is simple and robust, as well as flexible and adaptable to a variety of cases. It results in an optimally thin specimen providing low background while maintaining minute amounts of mother liquor around the crystals. The 2 × 2 mm area enables the deposition of up to several microlitres of liquid. Imaging and visualization of the crystals are straightforward on the highly transparent membrane. Thanks to their affordable fabrication, these supports have the potential to become an attractive option for serial experiments at synchrotrons and free-electron lasers. </p>}},
  author       = {{Martiel, Isabelle and Beale, John H. and Karpik, Agnieszka and Huang, Chia Ying and Vera, Laura and Olieric, Natacha and Wranik, Maximilian and Tsai, Ching Ju and Mühle, Jonas and Aurelius, Oskar and John, Juliane and Högbom, Martin and Wang, Meitian and Marsh, May and Padeste, Celestino}},
  issn         = {{2059-7983}},
  keywords     = {{Fixed target; Sample supports; Serial crystallography; SFX}},
  language     = {{eng}},
  month        = {{09}},
  pages        = {{1153--1167}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Acta Crystallographica Section D: Structural Biology}},
  title        = {{Versatile microporous polymer-based supports for serial macromolecular crystallography}},
  url          = {{http://dx.doi.org/10.1107/S2059798321007324}},
  doi          = {{10.1107/S2059798321007324}},
  volume       = {{77}},
  year         = {{2021}},
}