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Osmotic Concentration of Zebrafish (Danio rerio) Body Fluids is Lower in Larvae than in Adults

Kozłowski, Tomasz M LU ; Jönsson, Mikael LU ; Ek, Fredrik LU ; Olsson, Roger LU and Kröger, Ronald H H LU (2018) In Zebrafish 15(1). p.9-14
Abstract

We intended to perform optical and structural measurements on larval zebrafish eyes at 5 days post fertilization, that is, the earliest age at which zebrafish show visually guided behavior. However, excised larval crystalline lenses deteriorated quickly if immersed in a medium that gives good results with adult lenses from a variety of fish species. We suspected that the larvae have body fluids of lower osmolality and tested a medium with 240 mOsm, which is 75% of the established adult value of 320 mOsm. The optical quality of freshly excised and immersed lenses was used to judge the osmotic matches. In addition, we tested how well the shape of the eye is preserved in fixatives of different osmolalities. In both cases, 240 mOsm produced... (More)

We intended to perform optical and structural measurements on larval zebrafish eyes at 5 days post fertilization, that is, the earliest age at which zebrafish show visually guided behavior. However, excised larval crystalline lenses deteriorated quickly if immersed in a medium that gives good results with adult lenses from a variety of fish species. We suspected that the larvae have body fluids of lower osmolality and tested a medium with 240 mOsm, which is 75% of the established adult value of 320 mOsm. The optical quality of freshly excised and immersed lenses was used to judge the osmotic matches. In addition, we tested how well the shape of the eye is preserved in fixatives of different osmolalities. In both cases, 240 mOsm produced the best results. Immersed lenses performed better and the fixed eyes had a more natural shape. Our findings indicate that zebrafish body fluids have lower osmolality in larvae than in adults. This is probably due to an unfavorable body surface-to-volume ratio and incompletely developed regulatory mechanisms. Body fluid osmolality deviating from the adult value has to be taken into account in optical and histological work.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
development, crystalline lens, fixation, fast Fourier transform, histology, osmolarity
in
Zebrafish
volume
15
issue
1
pages
6 pages
external identifiers
  • wos:000414397400001
  • scopus:85041328412
ISSN
1545-8547
DOI
language
English
LU publication?
yes
id
6f71dd76-4f77-4e83-a9d9-45dec211c79b
date added to LUP
2017-12-14 16:55:45
date last changed
2018-05-29 12:10:51
@article{6f71dd76-4f77-4e83-a9d9-45dec211c79b,
  abstract     = {<p>We intended to perform optical and structural measurements on larval zebrafish eyes at 5 days post fertilization, that is, the earliest age at which zebrafish show visually guided behavior. However, excised larval crystalline lenses deteriorated quickly if immersed in a medium that gives good results with adult lenses from a variety of fish species. We suspected that the larvae have body fluids of lower osmolality and tested a medium with 240 mOsm, which is 75% of the established adult value of 320 mOsm. The optical quality of freshly excised and immersed lenses was used to judge the osmotic matches. In addition, we tested how well the shape of the eye is preserved in fixatives of different osmolalities. In both cases, 240 mOsm produced the best results. Immersed lenses performed better and the fixed eyes had a more natural shape. Our findings indicate that zebrafish body fluids have lower osmolality in larvae than in adults. This is probably due to an unfavorable body surface-to-volume ratio and incompletely developed regulatory mechanisms. Body fluid osmolality deviating from the adult value has to be taken into account in optical and histological work.</p>},
  author       = {Kozłowski, Tomasz M and Jönsson, Mikael and Ek, Fredrik and Olsson, Roger and Kröger, Ronald H H},
  issn         = {1545-8547},
  keyword      = {development,crystalline lens,fixation,fast Fourier transform,histology,osmolarity},
  language     = {eng},
  month        = {02},
  number       = {1},
  pages        = {9--14},
  series       = {Zebrafish},
  title        = {Osmotic Concentration of Zebrafish (Danio rerio) Body Fluids is Lower in Larvae than in Adults},
  url          = {http://dx.doi.org/},
  volume       = {15},
  year         = {2018},
}