Osmotic Concentration of Zebrafish (Danio rerio) Body Fluids is Lower in Larvae than in Adults
Kozłowski, Tomasz M LU ; Jönsson, Mikael LU ; Ek, Fredrik LU ; Olsson, Roger LU
and Kröger, Ronald H H
LU
(2018)
In Zebrafish
15(1).
p.9-14
- Abstract
We intended to perform optical and structural measurements on larval zebrafish eyes at 5 days post fertilization, that is, the earliest age at which zebrafish show visually guided behavior. However, excised larval crystalline lenses deteriorated quickly if immersed in a medium that gives good results with adult lenses from a variety of fish species. We suspected that the larvae have body fluids of lower osmolality and tested a medium with 240 mOsm, which is 75% of the established adult value of 320 mOsm. The optical quality of freshly excised and immersed lenses was used to judge the osmotic matches. In addition, we tested how well the shape of the eye is preserved in fixatives of different osmolalities. In both cases, 240 mOsm produced... (More)
We intended to perform optical and structural measurements on larval zebrafish eyes at 5 days post fertilization, that is, the earliest age at which zebrafish show visually guided behavior. However, excised larval crystalline lenses deteriorated quickly if immersed in a medium that gives good results with adult lenses from a variety of fish species. We suspected that the larvae have body fluids of lower osmolality and tested a medium with 240 mOsm, which is 75% of the established adult value of 320 mOsm. The optical quality of freshly excised and immersed lenses was used to judge the osmotic matches. In addition, we tested how well the shape of the eye is preserved in fixatives of different osmolalities. In both cases, 240 mOsm produced the best results. Immersed lenses performed better and the fixed eyes had a more natural shape. Our findings indicate that zebrafish body fluids have lower osmolality in larvae than in adults. This is probably due to an unfavorable body surface-to-volume ratio and incompletely developed regulatory mechanisms. Body fluid osmolality deviating from the adult value has to be taken into account in optical and histological work.
(Less)
- author
- Kozłowski, Tomasz M
LU
; Jönsson, Mikael
LU
; Ek, Fredrik
LU
; Olsson, Roger
LU
and Kröger, Ronald H H
LU
- organization
- publishing date
- 2018-02-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- development, crystalline lens, fixation, fast Fourier transform, histology, osmolarity
- in
- Zebrafish
- volume
- 15
- issue
- 1
- pages
- 6 pages
- publisher
- Mary Ann Liebert, Inc.
- external identifiers
-
- pmid:29058563
- wos:000414397400001
- scopus:85041328412
- ISSN
- 1545-8547
- DOI
- 10.1089/zeb.2017.1504
- language
- English
- LU publication?
- yes
- id
- 6f71dd76-4f77-4e83-a9d9-45dec211c79b
- date added to LUP
- 2017-12-14 16:55:45
- date last changed
- 2024-04-15 00:42:18
@article{6f71dd76-4f77-4e83-a9d9-45dec211c79b,
abstract = {{<p>We intended to perform optical and structural measurements on larval zebrafish eyes at 5 days post fertilization, that is, the earliest age at which zebrafish show visually guided behavior. However, excised larval crystalline lenses deteriorated quickly if immersed in a medium that gives good results with adult lenses from a variety of fish species. We suspected that the larvae have body fluids of lower osmolality and tested a medium with 240 mOsm, which is 75% of the established adult value of 320 mOsm. The optical quality of freshly excised and immersed lenses was used to judge the osmotic matches. In addition, we tested how well the shape of the eye is preserved in fixatives of different osmolalities. In both cases, 240 mOsm produced the best results. Immersed lenses performed better and the fixed eyes had a more natural shape. Our findings indicate that zebrafish body fluids have lower osmolality in larvae than in adults. This is probably due to an unfavorable body surface-to-volume ratio and incompletely developed regulatory mechanisms. Body fluid osmolality deviating from the adult value has to be taken into account in optical and histological work.</p>}},
author = {{Kozłowski, Tomasz M and Jönsson, Mikael and Ek, Fredrik and Olsson, Roger and Kröger, Ronald H H}},
issn = {{1545-8547}},
keywords = {{development; crystalline lens; fixation; fast Fourier transform; histology; osmolarity}},
language = {{eng}},
month = {{02}},
number = {{1}},
pages = {{9--14}},
publisher = {{Mary Ann Liebert, Inc.}},
series = {{Zebrafish}},
title = {{Osmotic Concentration of Zebrafish (Danio rerio) Body Fluids is Lower in Larvae than in Adults}},
url = {{http://dx.doi.org/10.1089/zeb.2017.1504}},
doi = {{10.1089/zeb.2017.1504}},
volume = {{15}},
year = {{2018}},
}