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Comparative Secretome Analyses of Primary Murine White and Brown Adipocytes Reveal Novel Adipokines

Ali Khan, Asrar; Hansson, Jenny LU ; Weber, Peter; Foehr, Sophia; Krijgsveld, Jeroen; Herzig, Stephan and Scheideler, Marcel (2018) In Molecular and Cellular Proteomics 17(12). p.2358-2370
Abstract

The adipose organ, including white and brown adipose tissues, is an important player in systemic energy homeostasis, storing excess energy in form of lipids while releasing energy upon various energy demands. Recent studies have demonstrated that white and brown adipocytes also function as endocrine cells and regulate systemic metabolism by secreting factors that act locally and systemically. However, a comparative proteomic analysis of secreted factors from white and brown adipocytes and their responsiveness to adrenergic stimulation has not been reported yet. Therefore, we studied and compared the secretome of white and brown adipocytes, with and without norepinephrine (NE) stimulation. Our results reveal that... (More)

The adipose organ, including white and brown adipose tissues, is an important player in systemic energy homeostasis, storing excess energy in form of lipids while releasing energy upon various energy demands. Recent studies have demonstrated that white and brown adipocytes also function as endocrine cells and regulate systemic metabolism by secreting factors that act locally and systemically. However, a comparative proteomic analysis of secreted factors from white and brown adipocytes and their responsiveness to adrenergic stimulation has not been reported yet. Therefore, we studied and compared the secretome of white and brown adipocytes, with and without norepinephrine (NE) stimulation. Our results reveal that carbohydrate-metabolism-regulating proteins are preferably secreted from white adipocytes, while brown adipocytes predominantly secrete a large variety of proteins. Upon NE stimulation, an increased secretion of known adipokines is favored by white adipocytes while brown adipocytes secreted higher amounts of novel adipokines. Furthermore, the secretory response between NE-stimulated and basal state was multifaceted addressing lipid and glucose metabolism, adipogenesis, and antioxidative reactions. Intriguingly, NE stimulation drastically changed the secretome in brown adipocytes. In conclusion, our study provides a comprehensive catalogue of novel adipokine candidates secreted from white and brown adipocytes with many of them responsive to NE. Given the beneficial effects of brown adipose tissue activation on its endocrine function and systemic metabolism, this study provides an archive of novel batokine candidates and biomarkers for activated brown adipose tissue.

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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular and Cellular Proteomics
volume
17
issue
12
pages
13 pages
publisher
American Society for Biochemistry and Molecular Biology
external identifiers
  • scopus:85057725719
ISSN
1535-9484
DOI
10.1074/mcp.RA118.000704
language
English
LU publication?
no
id
6fa087f4-4b3e-4145-a496-157c80d3f953
date added to LUP
2019-05-07 21:08:02
date last changed
2019-10-15 07:03:07
@article{6fa087f4-4b3e-4145-a496-157c80d3f953,
  abstract     = {<p>The adipose organ, including white and brown adipose tissues, is an important player in systemic energy homeostasis, storing excess energy in form of lipids while releasing energy upon various energy demands. Recent studies have demonstrated that white and brown adipocytes also function as endocrine cells and regulate systemic metabolism by secreting factors that act locally and systemically. However, a comparative proteomic analysis of secreted factors from white and brown adipocytes and their responsiveness to adrenergic stimulation has not been reported yet. Therefore, we studied and compared the secretome of white and brown adipocytes, with and without norepinephrine (NE) stimulation. Our results reveal that carbohydrate-metabolism-regulating proteins are preferably secreted from white adipocytes, while brown adipocytes predominantly secrete a large variety of proteins. Upon NE stimulation, an increased secretion of known adipokines is favored by white adipocytes while brown adipocytes secreted higher amounts of novel adipokines. Furthermore, the secretory response between NE-stimulated and basal state was multifaceted addressing lipid and glucose metabolism, adipogenesis, and antioxidative reactions. Intriguingly, NE stimulation drastically changed the secretome in brown adipocytes. In conclusion, our study provides a comprehensive catalogue of novel adipokine candidates secreted from white and brown adipocytes with many of them responsive to NE. Given the beneficial effects of brown adipose tissue activation on its endocrine function and systemic metabolism, this study provides an archive of novel batokine candidates and biomarkers for activated brown adipose tissue.</p>},
  author       = {Ali Khan, Asrar and Hansson, Jenny and Weber, Peter and Foehr, Sophia and Krijgsveld, Jeroen and Herzig, Stephan and Scheideler, Marcel},
  issn         = {1535-9484},
  language     = {eng},
  number       = {12},
  pages        = {2358--2370},
  publisher    = {American Society for Biochemistry and Molecular Biology},
  series       = {Molecular and Cellular Proteomics},
  title        = {Comparative Secretome Analyses of Primary Murine White and Brown Adipocytes Reveal Novel Adipokines},
  url          = {http://dx.doi.org/10.1074/mcp.RA118.000704},
  volume       = {17},
  year         = {2018},
}