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Healing of tympanic membrane after myringotomy during Streptococcus pneumoniae otitis media : An otomicroscopic and histologic study in the rat

Magnuson, Karin LU orcid ; Hermansson, Ann LU and Hellström, Sten (1996) In Annals of Otology, Rhinology and Laryngology 105(5). p.397-404
Abstract

The purpose of our study was to elucidate the course of healing of the tympanic membrane (TM) when myringotomy was performed during acute otitis media. The early and long-lasting structural changes of the TM were studied in an animal model. Rats were inoculated with Streptococcus pneumoniae (PnC) type 3 in the bulla. When the infection was manifest, myringotomy was performed. On days 4 and 12, and 3 and 6 months after myringotomy, the TM status was checked by otomicroscopy and TMs were prepared for light and electron microscopy. Comparison was made with PnC-infected TMs that were not perforated, as well as myringotomized noninfected TMs. The infection resolved more slowly in myringotomized ears compared to PnC-infected ears that were... (More)

The purpose of our study was to elucidate the course of healing of the tympanic membrane (TM) when myringotomy was performed during acute otitis media. The early and long-lasting structural changes of the TM were studied in an animal model. Rats were inoculated with Streptococcus pneumoniae (PnC) type 3 in the bulla. When the infection was manifest, myringotomy was performed. On days 4 and 12, and 3 and 6 months after myringotomy, the TM status was checked by otomicroscopy and TMs were prepared for light and electron microscopy. Comparison was made with PnC-infected TMs that were not perforated, as well as myringotomized noninfected TMs. The infection resolved more slowly in myringotomized ears compared to PnC-infected ears that were left untouched. After 6 months, the pars tensa of the myringotomized infected ears was thickened and showed a disorganized collagen structure, compared with myringotomized noninfected ears, in which TMs were normalized. The PnC- infected TMs without myringotomy were completely normalized after 2 months. We conclude that a combination of bacterial infection and myringotomy causes long-lasting changes in TM structure. This impaired structure of the connective tissue could be of importance in chronic middle ear disease as a presumptive site for retraction and perforation of the TM.

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author
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type
Contribution to journal
publication status
published
subject
keywords
myringotomy, rat, Streptococcus pneumoniae, tympanic membrane
in
Annals of Otology, Rhinology and Laryngology
volume
105
issue
5
pages
8 pages
publisher
Annals Publishing Company
external identifiers
  • pmid:8651635
  • scopus:0029927320
ISSN
0003-4894
DOI
10.1177/000348949610500513
language
English
LU publication?
yes
id
6fc10051-45cf-49c2-b71c-ef6bb8bf4b73
date added to LUP
2019-05-08 13:12:37
date last changed
2024-01-01 03:34:41
@article{6fc10051-45cf-49c2-b71c-ef6bb8bf4b73,
  abstract     = {{<p>The purpose of our study was to elucidate the course of healing of the tympanic membrane (TM) when myringotomy was performed during acute otitis media. The early and long-lasting structural changes of the TM were studied in an animal model. Rats were inoculated with Streptococcus pneumoniae (PnC) type 3 in the bulla. When the infection was manifest, myringotomy was performed. On days 4 and 12, and 3 and 6 months after myringotomy, the TM status was checked by otomicroscopy and TMs were prepared for light and electron microscopy. Comparison was made with PnC-infected TMs that were not perforated, as well as myringotomized noninfected TMs. The infection resolved more slowly in myringotomized ears compared to PnC-infected ears that were left untouched. After 6 months, the pars tensa of the myringotomized infected ears was thickened and showed a disorganized collagen structure, compared with myringotomized noninfected ears, in which TMs were normalized. The PnC- infected TMs without myringotomy were completely normalized after 2 months. We conclude that a combination of bacterial infection and myringotomy causes long-lasting changes in TM structure. This impaired structure of the connective tissue could be of importance in chronic middle ear disease as a presumptive site for retraction and perforation of the TM.</p>}},
  author       = {{Magnuson, Karin and Hermansson, Ann and Hellström, Sten}},
  issn         = {{0003-4894}},
  keywords     = {{myringotomy; rat; Streptococcus pneumoniae; tympanic membrane}},
  language     = {{eng}},
  month        = {{05}},
  number       = {{5}},
  pages        = {{397--404}},
  publisher    = {{Annals Publishing Company}},
  series       = {{Annals of Otology, Rhinology and Laryngology}},
  title        = {{Healing of tympanic membrane after myringotomy during Streptococcus pneumoniae otitis media : An otomicroscopic and histologic study in the rat}},
  url          = {{http://dx.doi.org/10.1177/000348949610500513}},
  doi          = {{10.1177/000348949610500513}},
  volume       = {{105}},
  year         = {{1996}},
}