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Ku protein in human T and B lymphocytes: full length functional form and signs of degradation

Sallmyr, Annahita LU ; Henriksson, Gunnel LU ; Fukushima, S and Bredberg, Anders LU (2001) In Biochimica et Biophysica Acta 1538(2-3). p.305-312
Abstract
DNA-dependent protein kinase (DNA-PK) has been shown to take part in cell cycle regulatory signal transduction and in the repair of X-ray-induced DNA double-strand breaks. Functional DNA-PK is furthermore needed for the generation of antigen specificity during lymphocyte maturation. The Ku86 subunit of DNA-PK has been reported to exist in human B lymphocytes in a truncated form capable of binding to broken DNA but lacking the ability to activate the kinase function of DNA-PK. In the present work the Ku70 and Ku86 dimer proteins in T and B lymphocytes from human blood donors were analysed by immunoblotting and were observed apparently to be of full length. Also, nuclear protein extracted from B and non-B lymphocytes displayed DNA-dependent... (More)
DNA-dependent protein kinase (DNA-PK) has been shown to take part in cell cycle regulatory signal transduction and in the repair of X-ray-induced DNA double-strand breaks. Functional DNA-PK is furthermore needed for the generation of antigen specificity during lymphocyte maturation. The Ku86 subunit of DNA-PK has been reported to exist in human B lymphocytes in a truncated form capable of binding to broken DNA but lacking the ability to activate the kinase function of DNA-PK. In the present work the Ku70 and Ku86 dimer proteins in T and B lymphocytes from human blood donors were analysed by immunoblotting and were observed apparently to be of full length. Also, nuclear protein extracted from B and non-B lymphocytes displayed DNA-dependent kinase activity. However, a minor fraction of Ku86 in lymphocytes was observed to be truncated with a molecular mass of approx. 70 kDa. (Less)
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Ku protein, DNA-dependent protein kinase, B lymphocyte, T lymphocyte
in
Biochimica et Biophysica Acta
volume
1538
issue
2-3
pages
305 - 312
publisher
Elsevier
external identifiers
  • pmid:11336801
  • scopus:0035938186
ISSN
0006-3002
DOI
10.1016/S0167-4889(01)00081-7
language
English
LU publication?
yes
id
70154637-e2f0-4550-8ee5-e8c95af1d8c6 (old id 1121601)
date added to LUP
2016-04-01 15:54:11
date last changed
2022-01-28 07:55:03
@article{70154637-e2f0-4550-8ee5-e8c95af1d8c6,
  abstract     = {{DNA-dependent protein kinase (DNA-PK) has been shown to take part in cell cycle regulatory signal transduction and in the repair of X-ray-induced DNA double-strand breaks. Functional DNA-PK is furthermore needed for the generation of antigen specificity during lymphocyte maturation. The Ku86 subunit of DNA-PK has been reported to exist in human B lymphocytes in a truncated form capable of binding to broken DNA but lacking the ability to activate the kinase function of DNA-PK. In the present work the Ku70 and Ku86 dimer proteins in T and B lymphocytes from human blood donors were analysed by immunoblotting and were observed apparently to be of full length. Also, nuclear protein extracted from B and non-B lymphocytes displayed DNA-dependent kinase activity. However, a minor fraction of Ku86 in lymphocytes was observed to be truncated with a molecular mass of approx. 70 kDa.}},
  author       = {{Sallmyr, Annahita and Henriksson, Gunnel and Fukushima, S and Bredberg, Anders}},
  issn         = {{0006-3002}},
  keywords     = {{Ku protein; DNA-dependent protein kinase; B lymphocyte; T lymphocyte}},
  language     = {{eng}},
  number       = {{2-3}},
  pages        = {{305--312}},
  publisher    = {{Elsevier}},
  series       = {{Biochimica et Biophysica Acta}},
  title        = {{Ku protein in human T and B lymphocytes: full length functional form and signs of degradation}},
  url          = {{http://dx.doi.org/10.1016/S0167-4889(01)00081-7}},
  doi          = {{10.1016/S0167-4889(01)00081-7}},
  volume       = {{1538}},
  year         = {{2001}},
}