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Fungal biomass production and turnover in soil estimated using the acetate-in-ergosterol technique

Rousk, Johannes LU and Bååth, Erland LU (2007) In Soil Biology & Biochemistry 39(8). p.2173-2177
Abstract
We report the first attempt to estimate fungal biomass production in soil by correlating relative fungal growth rates (i.e., acetate incorporation into ergosterol) with fungal biomass increase (i.e., ergosterol) following amendments with dried alfalfa or barley straw in soil. The conversion factor obtained was then used in unamended soil, resulting in fungal biomass productions of 10–12 μg C g−1 soil, yielding fungal turnover times between 130 and 150 days. Using a conversion factor from alfalfa-treated soil only resulted in two times higher estimates for biomass production and consequently lower turnover times. Comparing fungal biomass production with basal respiration indicated that these calculations overestimated the former. Still, the... (More)
We report the first attempt to estimate fungal biomass production in soil by correlating relative fungal growth rates (i.e., acetate incorporation into ergosterol) with fungal biomass increase (i.e., ergosterol) following amendments with dried alfalfa or barley straw in soil. The conversion factor obtained was then used in unamended soil, resulting in fungal biomass productions of 10–12 μg C g−1 soil, yielding fungal turnover times between 130 and 150 days. Using a conversion factor from alfalfa-treated soil only resulted in two times higher estimates for biomass production and consequently lower turnover times. Comparing fungal biomass production with basal respiration indicated that these calculations overestimated the former. Still, the turnover times of fungal biomass in soil were in the same range as turnover times estimated in aquatic systems. The slow turnover of fungal biomass contrasts with the short turnover times found for bacteria. The study thus presents empirical data substantiating the theoretical division of bacteria and fungi into a fast and a slow energy channel, respectively, in the soil food web. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Biomass production, Soil, Fungi, Ergosterol, 14C acetate
in
Soil Biology & Biochemistry
volume
39
issue
8
pages
2173 - 2177
publisher
Elsevier
external identifiers
  • wos:000247295800035
  • scopus:34248587936
ISSN
0038-0717
DOI
10.1016/j.soilbio.2007.03.023
project
Effect of environmental factors on fungal and bacterial growth in soil
language
English
LU publication?
yes
id
dd9e8e1e-5251-4716-951a-b9b0951108f2 (old id 707510)
date added to LUP
2007-12-11 12:48:34
date last changed
2017-10-08 04:25:10
@article{dd9e8e1e-5251-4716-951a-b9b0951108f2,
  abstract     = {We report the first attempt to estimate fungal biomass production in soil by correlating relative fungal growth rates (i.e., acetate incorporation into ergosterol) with fungal biomass increase (i.e., ergosterol) following amendments with dried alfalfa or barley straw in soil. The conversion factor obtained was then used in unamended soil, resulting in fungal biomass productions of 10–12 μg C g−1 soil, yielding fungal turnover times between 130 and 150 days. Using a conversion factor from alfalfa-treated soil only resulted in two times higher estimates for biomass production and consequently lower turnover times. Comparing fungal biomass production with basal respiration indicated that these calculations overestimated the former. Still, the turnover times of fungal biomass in soil were in the same range as turnover times estimated in aquatic systems. The slow turnover of fungal biomass contrasts with the short turnover times found for bacteria. The study thus presents empirical data substantiating the theoretical division of bacteria and fungi into a fast and a slow energy channel, respectively, in the soil food web.},
  author       = {Rousk, Johannes and Bååth, Erland},
  issn         = {0038-0717},
  keyword      = {Biomass production,Soil,Fungi,Ergosterol,14C acetate},
  language     = {eng},
  number       = {8},
  pages        = {2173--2177},
  publisher    = {Elsevier},
  series       = {Soil Biology & Biochemistry},
  title        = {Fungal biomass production and turnover in soil estimated using the acetate-in-ergosterol technique},
  url          = {http://dx.doi.org/10.1016/j.soilbio.2007.03.023},
  volume       = {39},
  year         = {2007},
}