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Composition of human faecal microbiota in resistance to Campylobacter infection

Kampmann, C. LU ; Dicksved, J.; Engstrand, L. and Rautelin, H. (2016) In Clinical Microbiology and Infection 22(1). p.1-61
Abstract

In mice, specific species composition of gut microbiota enhances susceptibility to Campylobacter jejuni but little is known about the specific composition of the human gut microbiota in providing protection from infections caused by enteropathogens. Healthy adult individuals, who travelled in groups from Sweden to destinations with an estimated high risk for acquisition of Campylobacter infection, were enrolled. Faecal samples, collected before travelling and after returning home, were cultured for bacterial enteropathogens, and analysed for Campylobacter by PCR and for the species composition of the microbiota by 16S amplicon massive parallel sequencing. The microbiota compositions were compared between persons who became infected... (More)

In mice, specific species composition of gut microbiota enhances susceptibility to Campylobacter jejuni but little is known about the specific composition of the human gut microbiota in providing protection from infections caused by enteropathogens. Healthy adult individuals, who travelled in groups from Sweden to destinations with an estimated high risk for acquisition of Campylobacter infection, were enrolled. Faecal samples, collected before travelling and after returning home, were cultured for bacterial enteropathogens, and analysed for Campylobacter by PCR and for the species composition of the microbiota by 16S amplicon massive parallel sequencing. The microbiota compositions were compared between persons who became infected during their travel and those who did not. A total of 63 participants completed the study; 14 became infected with Campylobacter, two with Salmonella and 47 remained negative for the enteropathogens tested. After exclusion of samples taken after antimicrobial treatment, 49 individuals were included in the final analyses. Intra-individual stability of the microbiota was demonstrated for samples taken before travelling. The original diversity of the faecal microbiota was significantly lower among individuals who later became infected compared with those who remained uninfected. The relative abundances of bacteria belonging to the family Lachnospiraceae, and more specifically its two genera Dorea and Coprococcus, were significantly higher among those who remained uninfected. The travel-related infection did not significantly modify the faecal microbiota composition. Species composition of human gut microbiota is important for colonization resistance to Campylobacter infection. Especially individuals with a lower diversity are more susceptible to Campylobacter infection.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Campylobacter, Colonization resistance, Enteritis, Infection, Microbiota
in
Clinical Microbiology and Infection
volume
22
issue
1
pages
1 - 61
publisher
Wiley-Blackwell
external identifiers
  • scopus:84959553385
ISSN
1198-743X
DOI
10.1016/j.cmi.2015.09.004
language
English
LU publication?
yes
id
707f9a6e-b139-4d3b-acec-c6220227ab95
date added to LUP
2016-05-10 08:25:46
date last changed
2017-07-23 05:14:11
@article{707f9a6e-b139-4d3b-acec-c6220227ab95,
  abstract     = {<p>In mice, specific species composition of gut microbiota enhances susceptibility to Campylobacter jejuni but little is known about the specific composition of the human gut microbiota in providing protection from infections caused by enteropathogens. Healthy adult individuals, who travelled in groups from Sweden to destinations with an estimated high risk for acquisition of Campylobacter infection, were enrolled. Faecal samples, collected before travelling and after returning home, were cultured for bacterial enteropathogens, and analysed for Campylobacter by PCR and for the species composition of the microbiota by 16S amplicon massive parallel sequencing. The microbiota compositions were compared between persons who became infected during their travel and those who did not. A total of 63 participants completed the study; 14 became infected with Campylobacter, two with Salmonella and 47 remained negative for the enteropathogens tested. After exclusion of samples taken after antimicrobial treatment, 49 individuals were included in the final analyses. Intra-individual stability of the microbiota was demonstrated for samples taken before travelling. The original diversity of the faecal microbiota was significantly lower among individuals who later became infected compared with those who remained uninfected. The relative abundances of bacteria belonging to the family Lachnospiraceae, and more specifically its two genera Dorea and Coprococcus, were significantly higher among those who remained uninfected. The travel-related infection did not significantly modify the faecal microbiota composition. Species composition of human gut microbiota is important for colonization resistance to Campylobacter infection. Especially individuals with a lower diversity are more susceptible to Campylobacter infection.</p>},
  author       = {Kampmann, C. and Dicksved, J. and Engstrand, L. and Rautelin, H.},
  issn         = {1198-743X},
  keyword      = {Campylobacter,Colonization resistance,Enteritis,Infection,Microbiota},
  language     = {eng},
  number       = {1},
  pages        = {1--61},
  publisher    = {Wiley-Blackwell},
  series       = {Clinical Microbiology and Infection},
  title        = {Composition of human faecal microbiota in resistance to Campylobacter infection},
  url          = {http://dx.doi.org/10.1016/j.cmi.2015.09.004},
  volume       = {22},
  year         = {2016},
}