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Flow cytometric parameters for characterizing platelet activation by measuring P-selectin (CD62) expression : Theoretical consideration and evaluation in thrombin-treated platelet populations

Leytin, Valery ; Mody, Meera ; Semple, John W. LU ; Garvey, Bernadette and Freedman, John (2000) In Biochemical and Biophysical Research Communications 269(1). p.85-90
Abstract

Two flow cytometric parameters are generally used to quantify platelet activation as measured by P-selectin (CD62) expression: percentage and mean channel fluorescence of CD62-positive platelets (%+ and MCF+, respectively). We describe a method for calculation of indices of platelet activation for positive (IPA+) and total (IPA(Σ)) platelets, which reflect integrated amounts of CD62 expressed in these populations; IPA+ is calculated as the product of %+ and MCF+, whereas IPA(Σ) is exclusively determined by mean fluorescence of the total platelet population (MCF(Σ)) and does not depend on %+. We use these parameters to characterize human platelet activation in... (More)

Two flow cytometric parameters are generally used to quantify platelet activation as measured by P-selectin (CD62) expression: percentage and mean channel fluorescence of CD62-positive platelets (%+ and MCF+, respectively). We describe a method for calculation of indices of platelet activation for positive (IPA+) and total (IPA(Σ)) platelets, which reflect integrated amounts of CD62 expressed in these populations; IPA+ is calculated as the product of %+ and MCF+, whereas IPA(Σ) is exclusively determined by mean fluorescence of the total platelet population (MCF(Σ)) and does not depend on %+. We use these parameters to characterize human platelet activation in whole blood samples treated with varying human α-thrombin concentrations, mimicking the variations in platelet activation in a number of clinical settings. Multiparameter analysis of CD62 expression may be useful for selective diagnosis of disorders with systemic or localized platelet activation and for monitoring the clinical course of the disease and effect of therapeutic interventions. (C) 2000 Academic Press.

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author
; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Flow cytometric parameters, Human α-thrombin, P-selectin (CD62) expression, Platelet activation
in
Biochemical and Biophysical Research Communications
volume
269
issue
1
pages
6 pages
publisher
Elsevier
external identifiers
  • pmid:10694482
  • scopus:0034607136
ISSN
0006-291X
DOI
10.1006/bbrc.2000.2255
language
English
LU publication?
no
id
70c590b4-a1c1-438e-bf7c-53bf92e024d7
date added to LUP
2019-12-03 10:26:58
date last changed
2024-03-20 01:34:08
@article{70c590b4-a1c1-438e-bf7c-53bf92e024d7,
  abstract     = {{<p>Two flow cytometric parameters are generally used to quantify platelet activation as measured by P-selectin (CD62) expression: percentage and mean channel fluorescence of CD62-positive platelets (%<sup>+</sup> and MCF<sup>+</sup>, respectively). We describe a method for calculation of indices of platelet activation for positive (IPA<sup>+</sup>) and total (IPA(Σ)) platelets, which reflect integrated amounts of CD62 expressed in these populations; IPA<sup>+</sup> is calculated as the product of %<sup>+</sup> and MCF<sup>+</sup>, whereas IPA(Σ) is exclusively determined by mean fluorescence of the total platelet population (MCF(Σ)) and does not depend on %<sup>+</sup>. We use these parameters to characterize human platelet activation in whole blood samples treated with varying human α-thrombin concentrations, mimicking the variations in platelet activation in a number of clinical settings. Multiparameter analysis of CD62 expression may be useful for selective diagnosis of disorders with systemic or localized platelet activation and for monitoring the clinical course of the disease and effect of therapeutic interventions. (C) 2000 Academic Press.</p>}},
  author       = {{Leytin, Valery and Mody, Meera and Semple, John W. and Garvey, Bernadette and Freedman, John}},
  issn         = {{0006-291X}},
  keywords     = {{Flow cytometric parameters; Human α-thrombin; P-selectin (CD62) expression; Platelet activation}},
  language     = {{eng}},
  month        = {{03}},
  number       = {{1}},
  pages        = {{85--90}},
  publisher    = {{Elsevier}},
  series       = {{Biochemical and Biophysical Research Communications}},
  title        = {{Flow cytometric parameters for characterizing platelet activation by measuring P-selectin (CD62) expression : Theoretical consideration and evaluation in thrombin-treated platelet populations}},
  url          = {{http://dx.doi.org/10.1006/bbrc.2000.2255}},
  doi          = {{10.1006/bbrc.2000.2255}},
  volume       = {{269}},
  year         = {{2000}},
}