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Membrane interactions and antimicrobial effects of layered double hydroxide nanoparticles

Malekkhaiat-Häffner, S.; Nyström, L.; Nordström, Randi; Xu, Z. P.; Davoudi, M. LU ; Schmidtchen, A. LU and Malmsten, M. (2017) In Physical Chemistry Chemical Physics 19(35). p.23832-23842
Abstract

Membrane interactions are critical for the successful use of inorganic nanoparticles as antimicrobial agents and as carriers of, or co-actives with, antimicrobial peptides (AMPs). In order to contribute to an increased understanding of these, we here investigate effects of particle size (42-208 nm) on layered double hydroxide (LDH) interactions with both bacteria-mimicking and mammalian-mimicking lipid membranes. LDH binding to bacteria-mimicking membranes, extraction of anionic lipids, as well as resulting membrane destabilization, was found to increase with decreasing particle size, also translating into size-dependent synergistic effects with the antimicrobial peptide LL-37. Due to strong interactions with anionic lipopolysaccharide... (More)

Membrane interactions are critical for the successful use of inorganic nanoparticles as antimicrobial agents and as carriers of, or co-actives with, antimicrobial peptides (AMPs). In order to contribute to an increased understanding of these, we here investigate effects of particle size (42-208 nm) on layered double hydroxide (LDH) interactions with both bacteria-mimicking and mammalian-mimicking lipid membranes. LDH binding to bacteria-mimicking membranes, extraction of anionic lipids, as well as resulting membrane destabilization, was found to increase with decreasing particle size, also translating into size-dependent synergistic effects with the antimicrobial peptide LL-37. Due to strong interactions with anionic lipopolysaccharide and peptidoglycan layers, direct membrane disruption of both Gram-negative and Gram-positive bacteria is suppressed. However, LDH nanoparticles cause size-dependent charge reversal and resulting flocculation of both liposomes and bacteria, which may provide a mechanism for bacterial confinement or clearance. Taken together, these findings demonstrate a set of previously unknown behaviors, including synergistic membrane destabilization and dual confinement/killing of bacteria through combined LDH/AMP exposure, of potential therapeutic interest.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Physical Chemistry Chemical Physics
volume
19
issue
35
pages
11 pages
publisher
Royal Society of Chemistry
external identifiers
  • scopus:85029555120
  • wos:000410585900026
ISSN
1463-9076
DOI
10.1039/c7cp02701j
language
English
LU publication?
yes
id
70e323fd-0a35-4eed-9707-69e14c5f8b22
date added to LUP
2017-10-05 08:11:06
date last changed
2018-01-16 13:20:54
@article{70e323fd-0a35-4eed-9707-69e14c5f8b22,
  abstract     = {<p>Membrane interactions are critical for the successful use of inorganic nanoparticles as antimicrobial agents and as carriers of, or co-actives with, antimicrobial peptides (AMPs). In order to contribute to an increased understanding of these, we here investigate effects of particle size (42-208 nm) on layered double hydroxide (LDH) interactions with both bacteria-mimicking and mammalian-mimicking lipid membranes. LDH binding to bacteria-mimicking membranes, extraction of anionic lipids, as well as resulting membrane destabilization, was found to increase with decreasing particle size, also translating into size-dependent synergistic effects with the antimicrobial peptide LL-37. Due to strong interactions with anionic lipopolysaccharide and peptidoglycan layers, direct membrane disruption of both Gram-negative and Gram-positive bacteria is suppressed. However, LDH nanoparticles cause size-dependent charge reversal and resulting flocculation of both liposomes and bacteria, which may provide a mechanism for bacterial confinement or clearance. Taken together, these findings demonstrate a set of previously unknown behaviors, including synergistic membrane destabilization and dual confinement/killing of bacteria through combined LDH/AMP exposure, of potential therapeutic interest.</p>},
  author       = {Malekkhaiat-Häffner, S. and Nyström, L. and Nordström, Randi and Xu, Z. P. and Davoudi, M. and Schmidtchen, A. and Malmsten, M.},
  issn         = {1463-9076},
  language     = {eng},
  number       = {35},
  pages        = {23832--23842},
  publisher    = {Royal Society of Chemistry},
  series       = {Physical Chemistry Chemical Physics},
  title        = {Membrane interactions and antimicrobial effects of layered double hydroxide nanoparticles},
  url          = {http://dx.doi.org/10.1039/c7cp02701j},
  volume       = {19},
  year         = {2017},
}