Membrane interactions and antimicrobial effects of layered double hydroxide nanoparticles
Malekkhaiat-Häffner, S. ; Nyström, L. ; Nordström, Randi ; Xu, Z. P. ; Davoudi, M. LU
; Schmidtchen, A.
LU
and Malmsten, M.
(2017)
In Physical Chemistry Chemical Physics
19(35).
p.23832-23842
- Abstract
Membrane interactions are critical for the successful use of inorganic nanoparticles as antimicrobial agents and as carriers of, or co-actives with, antimicrobial peptides (AMPs). In order to contribute to an increased understanding of these, we here investigate effects of particle size (42-208 nm) on layered double hydroxide (LDH) interactions with both bacteria-mimicking and mammalian-mimicking lipid membranes. LDH binding to bacteria-mimicking membranes, extraction of anionic lipids, as well as resulting membrane destabilization, was found to increase with decreasing particle size, also translating into size-dependent synergistic effects with the antimicrobial peptide LL-37. Due to strong interactions with anionic lipopolysaccharide... (More)
Membrane interactions are critical for the successful use of inorganic nanoparticles as antimicrobial agents and as carriers of, or co-actives with, antimicrobial peptides (AMPs). In order to contribute to an increased understanding of these, we here investigate effects of particle size (42-208 nm) on layered double hydroxide (LDH) interactions with both bacteria-mimicking and mammalian-mimicking lipid membranes. LDH binding to bacteria-mimicking membranes, extraction of anionic lipids, as well as resulting membrane destabilization, was found to increase with decreasing particle size, also translating into size-dependent synergistic effects with the antimicrobial peptide LL-37. Due to strong interactions with anionic lipopolysaccharide and peptidoglycan layers, direct membrane disruption of both Gram-negative and Gram-positive bacteria is suppressed. However, LDH nanoparticles cause size-dependent charge reversal and resulting flocculation of both liposomes and bacteria, which may provide a mechanism for bacterial confinement or clearance. Taken together, these findings demonstrate a set of previously unknown behaviors, including synergistic membrane destabilization and dual confinement/killing of bacteria through combined LDH/AMP exposure, of potential therapeutic interest.
(Less)
- author
- Malekkhaiat-Häffner, S.
; Nyström, L.
; Nordström, Randi
; Xu, Z. P.
; Davoudi, M.
LU
; Schmidtchen, A.
LU
and Malmsten, M.
- organization
- publishing date
- 2017
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Physical Chemistry Chemical Physics
- volume
- 19
- issue
- 35
- pages
- 11 pages
- publisher
- Royal Society of Chemistry
- external identifiers
-
- scopus:85029555120
- pmid:28682360
- wos:000410585900026
- ISSN
- 1463-9076
- DOI
- 10.1039/c7cp02701j
- language
- English
- LU publication?
- yes
- id
- 70e323fd-0a35-4eed-9707-69e14c5f8b22
- date added to LUP
- 2017-10-05 08:11:06
- date last changed
- 2024-06-10 00:44:32
@article{70e323fd-0a35-4eed-9707-69e14c5f8b22,
abstract = {{<p>Membrane interactions are critical for the successful use of inorganic nanoparticles as antimicrobial agents and as carriers of, or co-actives with, antimicrobial peptides (AMPs). In order to contribute to an increased understanding of these, we here investigate effects of particle size (42-208 nm) on layered double hydroxide (LDH) interactions with both bacteria-mimicking and mammalian-mimicking lipid membranes. LDH binding to bacteria-mimicking membranes, extraction of anionic lipids, as well as resulting membrane destabilization, was found to increase with decreasing particle size, also translating into size-dependent synergistic effects with the antimicrobial peptide LL-37. Due to strong interactions with anionic lipopolysaccharide and peptidoglycan layers, direct membrane disruption of both Gram-negative and Gram-positive bacteria is suppressed. However, LDH nanoparticles cause size-dependent charge reversal and resulting flocculation of both liposomes and bacteria, which may provide a mechanism for bacterial confinement or clearance. Taken together, these findings demonstrate a set of previously unknown behaviors, including synergistic membrane destabilization and dual confinement/killing of bacteria through combined LDH/AMP exposure, of potential therapeutic interest.</p>}},
author = {{Malekkhaiat-Häffner, S. and Nyström, L. and Nordström, Randi and Xu, Z. P. and Davoudi, M. and Schmidtchen, A. and Malmsten, M.}},
issn = {{1463-9076}},
language = {{eng}},
number = {{35}},
pages = {{23832--23842}},
publisher = {{Royal Society of Chemistry}},
series = {{Physical Chemistry Chemical Physics}},
title = {{Membrane interactions and antimicrobial effects of layered double hydroxide nanoparticles}},
url = {{http://dx.doi.org/10.1039/c7cp02701j}},
doi = {{10.1039/c7cp02701j}},
volume = {{19}},
year = {{2017}},
}