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Theranostic hexosomes for cancer treatments : an in vitro study

Meli, Valeri; Caltagirone, Claudia; Sinico, Chiara; Lai, Francesco; Falchi, Angela M; Monduzzi, Maura; Obiols-Rabasa, Marc LU ; Picci, Giacomo; Rosa, Antonella and Schmidt, Judith, et al. (2017) In New Journal of Chemistry 41(4). p.1558-1565
Abstract

We have formulated and investigated innovative lipid-based nanoparticles characterized by a reverse hexagonal liquid crystalline inner structure (hexosomes). These nanoparticles were doped with a potent, highly water insoluble anticancer drug, namely docetaxel, and stabilized by a mixture of commercial and folate- and rhodamine-conjugated Pluronic F108. Thus, they simultaneously possess therapeutic, imaging, and targeting properties toward cancer cells. The morphological and structural aspects of the hexosomes were investigated at different temperatures (10, 25, 37, and 50 °C), and our results demonstrate good performance in terms of stability of these nanoparticles. The latter was furthermore confirmed by the very slow and continuous... (More)

We have formulated and investigated innovative lipid-based nanoparticles characterized by a reverse hexagonal liquid crystalline inner structure (hexosomes). These nanoparticles were doped with a potent, highly water insoluble anticancer drug, namely docetaxel, and stabilized by a mixture of commercial and folate- and rhodamine-conjugated Pluronic F108. Thus, they simultaneously possess therapeutic, imaging, and targeting properties toward cancer cells. The morphological and structural aspects of the hexosomes were investigated at different temperatures (10, 25, 37, and 50 °C), and our results demonstrate good performance in terms of stability of these nanoparticles. The latter was furthermore confirmed by the very slow and continuous release profile of docetaxel observed in drug release experiments. Although it was not possible to assess a specific compartmentalization of the dye, this formulation allowed the successful visualization of HeLa cells. Finally, cytotoxic assays showed a 20-fold higher toxic effect of the drug-doped hexosomes against HeLa cells with respect to the free (not loaded in hexosomes) anticancer drug. On the whole, these results indicate that this formulation is a potential theranostic tool in oncology.

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Contribution to journal
publication status
published
subject
in
New Journal of Chemistry
volume
41
issue
4
pages
8 pages
publisher
Royal Society of Chemistry
external identifiers
  • scopus:85012879960
  • wos:000395092600018
ISSN
1144-0546
DOI
10.1039/c6nj03232j
language
English
LU publication?
yes
id
712667de-4754-42b5-baf6-002bea5eac81
date added to LUP
2017-03-01 13:58:04
date last changed
2018-01-14 04:30:40
@article{712667de-4754-42b5-baf6-002bea5eac81,
  abstract     = {<p>We have formulated and investigated innovative lipid-based nanoparticles characterized by a reverse hexagonal liquid crystalline inner structure (hexosomes). These nanoparticles were doped with a potent, highly water insoluble anticancer drug, namely docetaxel, and stabilized by a mixture of commercial and folate- and rhodamine-conjugated Pluronic F108. Thus, they simultaneously possess therapeutic, imaging, and targeting properties toward cancer cells. The morphological and structural aspects of the hexosomes were investigated at different temperatures (10, 25, 37, and 50 °C), and our results demonstrate good performance in terms of stability of these nanoparticles. The latter was furthermore confirmed by the very slow and continuous release profile of docetaxel observed in drug release experiments. Although it was not possible to assess a specific compartmentalization of the dye, this formulation allowed the successful visualization of HeLa cells. Finally, cytotoxic assays showed a 20-fold higher toxic effect of the drug-doped hexosomes against HeLa cells with respect to the free (not loaded in hexosomes) anticancer drug. On the whole, these results indicate that this formulation is a potential theranostic tool in oncology.</p>},
  author       = {Meli, Valeri and Caltagirone, Claudia and Sinico, Chiara and Lai, Francesco and Falchi, Angela M and Monduzzi, Maura and Obiols-Rabasa, Marc and Picci, Giacomo and Rosa, Antonella and Schmidt, Judith and Talmon, Yeshayahu and Murgia, Sergio},
  issn         = {1144-0546},
  language     = {eng},
  number       = {4},
  pages        = {1558--1565},
  publisher    = {Royal Society of Chemistry},
  series       = {New Journal of Chemistry},
  title        = {Theranostic hexosomes for cancer treatments : an in vitro study},
  url          = {http://dx.doi.org/10.1039/c6nj03232j},
  volume       = {41},
  year         = {2017},
}