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Immune responsiveness against allogeneic platelet transfusions is determined by the recipient's major histocompatibility complex class II phenotype

Sayeh, Ebrahim ; Aslam, Rukshana ; Speck, Edwin R. ; Le-Tien, Hoang ; Lazarus, Alan H. ; Freedman, John and Semple, John W. LU (2004) In Transfusion 44(11). p.1572-1578
Abstract

BACKGROUND: Immunoglobulin G (IgG) anti-platelet (PLT) immunity has been shown to be initiated by indirect allorecognition where recipient T cells recognize donor PLT antigens presented by class II molecules encoded by the major histocompatibility complex (MHC) on recipient antigen-presenting cells. To understand how the recipient's MHC class II molecules may influence PLT alloimmunity, immune responsiveness against transfused PLTs was tested in different mouse strains. STUDY DESIGN AMD METHODS: Various inbred and mutant mouse strains were transfused with allogeneic PLTs and IgG donor antibodies were measured by flow cytometry. RESULTS: When recipient mice, expressing both MHC class II I-A and MHC class II I-E molecules, were transfused... (More)

BACKGROUND: Immunoglobulin G (IgG) anti-platelet (PLT) immunity has been shown to be initiated by indirect allorecognition where recipient T cells recognize donor PLT antigens presented by class II molecules encoded by the major histocompatibility complex (MHC) on recipient antigen-presenting cells. To understand how the recipient's MHC class II molecules may influence PLT alloimmunity, immune responsiveness against transfused PLTs was tested in different mouse strains. STUDY DESIGN AMD METHODS: Various inbred and mutant mouse strains were transfused with allogeneic PLTs and IgG donor antibodies were measured by flow cytometry. RESULTS: When recipient mice, expressing both MHC class II I-A and MHC class II I-E molecules, were transfused weekly with allogeneic PLTs, high titers of IgG donor antibodies were generated. In comparison, however, recipient mice expressing only MHC class II I-A molecules had significantly (p < 0.001) reduced IgG antibody responsiveness against PLT transfusions. The low IgG responder status against allogeneic PLT transfusions was rescued in transgenic mice expressing I-E molecules and in mice genetically deficient in either β2-microglobulin or CD8+ T cells. CONCLUSION: IgG immune responsiveness against allogeneic PLT transfusions is dependent on recipient expression of I-E MHC class II molecules, whereas I-A expression is linked with CD8-mediated suppression of PLT immunity. The data suggest that strategies to modify recipient MHC class II presentation of donor PLT antigens would be effective in eliminating PLT alloimmunity.

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author
; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
in
Transfusion
volume
44
issue
11
pages
1572 - 1578
publisher
Wiley-Blackwell
external identifiers
  • scopus:7244260718
  • pmid:15504162
ISSN
0041-1132
DOI
10.1111/j.1537-2995.2004.04197.x
language
English
LU publication?
no
id
7165ff23-8355-427a-974e-fc5464e90981
date added to LUP
2019-12-03 10:21:26
date last changed
2024-01-02 01:03:49
@article{7165ff23-8355-427a-974e-fc5464e90981,
  abstract     = {{<p>BACKGROUND: Immunoglobulin G (IgG) anti-platelet (PLT) immunity has been shown to be initiated by indirect allorecognition where recipient T cells recognize donor PLT antigens presented by class II molecules encoded by the major histocompatibility complex (MHC) on recipient antigen-presenting cells. To understand how the recipient's MHC class II molecules may influence PLT alloimmunity, immune responsiveness against transfused PLTs was tested in different mouse strains. STUDY DESIGN AMD METHODS: Various inbred and mutant mouse strains were transfused with allogeneic PLTs and IgG donor antibodies were measured by flow cytometry. RESULTS: When recipient mice, expressing both MHC class II I-A and MHC class II I-E molecules, were transfused weekly with allogeneic PLTs, high titers of IgG donor antibodies were generated. In comparison, however, recipient mice expressing only MHC class II I-A molecules had significantly (p &lt; 0.001) reduced IgG antibody responsiveness against PLT transfusions. The low IgG responder status against allogeneic PLT transfusions was rescued in transgenic mice expressing I-E molecules and in mice genetically deficient in either β<sub>2</sub>-microglobulin or CD8+ T cells. CONCLUSION: IgG immune responsiveness against allogeneic PLT transfusions is dependent on recipient expression of I-E MHC class II molecules, whereas I-A expression is linked with CD8-mediated suppression of PLT immunity. The data suggest that strategies to modify recipient MHC class II presentation of donor PLT antigens would be effective in eliminating PLT alloimmunity.</p>}},
  author       = {{Sayeh, Ebrahim and Aslam, Rukshana and Speck, Edwin R. and Le-Tien, Hoang and Lazarus, Alan H. and Freedman, John and Semple, John W.}},
  issn         = {{0041-1132}},
  language     = {{eng}},
  month        = {{11}},
  number       = {{11}},
  pages        = {{1572--1578}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Transfusion}},
  title        = {{Immune responsiveness against allogeneic platelet transfusions is determined by the recipient's major histocompatibility complex class II phenotype}},
  url          = {{http://dx.doi.org/10.1111/j.1537-2995.2004.04197.x}},
  doi          = {{10.1111/j.1537-2995.2004.04197.x}},
  volume       = {{44}},
  year         = {{2004}},
}