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Revised transmembrane orientation of the NADH:quinone oxidoreductase subunit NuoA.

Virzintiene, Egle LU ; Trane, Maria LU and Hägerhäll, Cecilia LU (2011) In FEBS Letters 585. p.3277-3283
Abstract
NuoA is a small membrane spanning subunit of respiratory chain NADH:quinone oxidoreductase (complex I). Unlike the other complex I core protein subunits, the NuoA protein has no known homologue in other enzyme systems. The transmembrane orientation of NuoA cannot be unambiguously predicted, due to the small size of the polypeptide and the varying distribution of charged amino acid residues in NuoA from different organisms. Novel analyses of NuoA from Escherichia coli complex I expressed as fusion proteins to cytochrome c and to alkaline phosphatase demonstrated that the c-terminal end of the polypeptide is localized in the bacterial cytoplasm, in contrast to what was previously reported for the homologous NQO7 subunit from Paracoccus... (More)
NuoA is a small membrane spanning subunit of respiratory chain NADH:quinone oxidoreductase (complex I). Unlike the other complex I core protein subunits, the NuoA protein has no known homologue in other enzyme systems. The transmembrane orientation of NuoA cannot be unambiguously predicted, due to the small size of the polypeptide and the varying distribution of charged amino acid residues in NuoA from different organisms. Novel analyses of NuoA from Escherichia coli complex I expressed as fusion proteins to cytochrome c and to alkaline phosphatase demonstrated that the c-terminal end of the polypeptide is localized in the bacterial cytoplasm, in contrast to what was previously reported for the homologous NQO7 subunit from Paracoccus denitrificans complex I. (Less)
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publishing date
type
Contribution to journal
publication status
published
subject
in
FEBS Letters
volume
585
pages
3277 - 3283
publisher
Wiley-Blackwell
external identifiers
  • wos:000296514100020
  • pmid:21925501
  • scopus:80054073803
ISSN
1873-3468
DOI
10.1016/j.febslet.2011.09.006
language
English
LU publication?
yes
id
718ab85c-013c-4e4c-89f5-081f503fb6ca (old id 2168823)
date added to LUP
2016-04-01 14:12:58
date last changed
2022-01-27 23:28:46
@article{718ab85c-013c-4e4c-89f5-081f503fb6ca,
  abstract     = {{NuoA is a small membrane spanning subunit of respiratory chain NADH:quinone oxidoreductase (complex I). Unlike the other complex I core protein subunits, the NuoA protein has no known homologue in other enzyme systems. The transmembrane orientation of NuoA cannot be unambiguously predicted, due to the small size of the polypeptide and the varying distribution of charged amino acid residues in NuoA from different organisms. Novel analyses of NuoA from Escherichia coli complex I expressed as fusion proteins to cytochrome c and to alkaline phosphatase demonstrated that the c-terminal end of the polypeptide is localized in the bacterial cytoplasm, in contrast to what was previously reported for the homologous NQO7 subunit from Paracoccus denitrificans complex I.}},
  author       = {{Virzintiene, Egle and Trane, Maria and Hägerhäll, Cecilia}},
  issn         = {{1873-3468}},
  language     = {{eng}},
  pages        = {{3277--3283}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{FEBS Letters}},
  title        = {{Revised transmembrane orientation of the NADH:quinone oxidoreductase subunit NuoA.}},
  url          = {{http://dx.doi.org/10.1016/j.febslet.2011.09.006}},
  doi          = {{10.1016/j.febslet.2011.09.006}},
  volume       = {{585}},
  year         = {{2011}},
}