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A multiscale analysis of early flower development in Arabidopsis provides an integrated view of molecular regulation and growth control

Refahi, Yassin ; Zardilis, Argyris ; Michelin, Gaël ; Wightman, Raymond ; Leggio, Bruno ; Legrand, Jonathan ; Faure, Emmanuel ; Vachez, Laetitia ; Armezzani, Alessia and Risson, Anne Evodie , et al. (2021) In Developmental Cell 56(4). p.8-556
Abstract

We have analyzed the link between the gene regulation and growth during the early stages of flower development in Arabidopsis. Starting from time-lapse images, we generated a 4D atlas of early flower development, including cell lineage, cellular growth rates, and the expression patterns of regulatory genes. This information was introduced in MorphoNet, a web-based platform. Using computational models, we found that the literature-based molecular network only explained a minority of the gene expression patterns. This was substantially improved by adding regulatory hypotheses for individual genes. Correlating growth with the combinatorial expression of multiple regulators led to a set of hypotheses for the action of individual genes in... (More)

We have analyzed the link between the gene regulation and growth during the early stages of flower development in Arabidopsis. Starting from time-lapse images, we generated a 4D atlas of early flower development, including cell lineage, cellular growth rates, and the expression patterns of regulatory genes. This information was introduced in MorphoNet, a web-based platform. Using computational models, we found that the literature-based molecular network only explained a minority of the gene expression patterns. This was substantially improved by adding regulatory hypotheses for individual genes. Correlating growth with the combinatorial expression of multiple regulators led to a set of hypotheses for the action of individual genes in morphogenesis. This identified the central factor LEAFY as a potential regulator of heterogeneous growth, which was supported by quantifying growth patterns in a leafy mutant. By providing an integrated view, this atlas should represent a fundamental step toward mechanistic models of flower development. Refahi and Zardilis et al. present a 4D atlas of early flower development. They combine growth and gene expression to construct a molecular network model that correctly predicts a large majority of 28 gene expression patterns. This model suggests hypotheses for the combined action of regulatory genes in morphogenesis.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
flower development, gene expression, molecular network, morphogenesis
in
Developmental Cell
volume
56
issue
4
pages
8 - 556
publisher
Cell Press
external identifiers
  • pmid:33621494
  • scopus:85101324934
ISSN
1534-5807
DOI
10.1016/j.devcel.2021.01.019
language
English
LU publication?
yes
id
7192fb39-cc66-4838-ab11-85841b73f1a1
date added to LUP
2021-03-09 11:19:50
date last changed
2025-02-07 08:50:54
@article{7192fb39-cc66-4838-ab11-85841b73f1a1,
  abstract     = {{<p>We have analyzed the link between the gene regulation and growth during the early stages of flower development in Arabidopsis. Starting from time-lapse images, we generated a 4D atlas of early flower development, including cell lineage, cellular growth rates, and the expression patterns of regulatory genes. This information was introduced in MorphoNet, a web-based platform. Using computational models, we found that the literature-based molecular network only explained a minority of the gene expression patterns. This was substantially improved by adding regulatory hypotheses for individual genes. Correlating growth with the combinatorial expression of multiple regulators led to a set of hypotheses for the action of individual genes in morphogenesis. This identified the central factor LEAFY as a potential regulator of heterogeneous growth, which was supported by quantifying growth patterns in a leafy mutant. By providing an integrated view, this atlas should represent a fundamental step toward mechanistic models of flower development. Refahi and Zardilis et al. present a 4D atlas of early flower development. They combine growth and gene expression to construct a molecular network model that correctly predicts a large majority of 28 gene expression patterns. This model suggests hypotheses for the combined action of regulatory genes in morphogenesis.</p>}},
  author       = {{Refahi, Yassin and Zardilis, Argyris and Michelin, Gaël and Wightman, Raymond and Leggio, Bruno and Legrand, Jonathan and Faure, Emmanuel and Vachez, Laetitia and Armezzani, Alessia and Risson, Anne Evodie and Zhao, Feng and Das, Pradeep and Prunet, Nathanaël and Meyerowitz, Elliot M. and Godin, Christophe and Malandain, Grégoire and Jönsson, Henrik and Traas, Jan}},
  issn         = {{1534-5807}},
  keywords     = {{flower development; gene expression; molecular network; morphogenesis}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{8--556}},
  publisher    = {{Cell Press}},
  series       = {{Developmental Cell}},
  title        = {{A multiscale analysis of early flower development in Arabidopsis provides an integrated view of molecular regulation and growth control}},
  url          = {{http://dx.doi.org/10.1016/j.devcel.2021.01.019}},
  doi          = {{10.1016/j.devcel.2021.01.019}},
  volume       = {{56}},
  year         = {{2021}},
}