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Prevalence and classification of meniscal calcifications in the human knee

Shakya, Bijay Ratna ; Karjalainen, Ville-Pauli ; Hellberg, Iida ; Finnilä, Mikko A J ; Elkhouly, Khaled ; Sjögren, Amanda LU orcid ; Turkiewicz, Aleksandra LU ; Önnerfjord, Patrik LU orcid ; Hughes, Velocity LU and Tjörnstrand, Jon LU , et al. (2024) In Osteoarthritis and Cartilage
Abstract

OBJECTIVE: To investigate the occurrence of meniscal calcifications in individuals with and without knee osteoarthritis (OA). Additionally, we aim to identify the specific types of calcifications: basic calcium phosphate (BCP) and calcium pyrophosphate dihydrate (CPP).

METHOD: We analysed 82 meniscal posterior horn samples (medial and lateral) collected from 41 human subjects. Among them, 20 individuals underwent total knee replacement due to medial compartment OA, while 21 deceased donors had no known knee OA. The assessment of meniscal calcifications and Pauli's histopathological scoring was conducted using histological sections. Furthermore, adjacent sections underwent measurement using Raman spectroscopy to characterize BCP... (More)

OBJECTIVE: To investigate the occurrence of meniscal calcifications in individuals with and without knee osteoarthritis (OA). Additionally, we aim to identify the specific types of calcifications: basic calcium phosphate (BCP) and calcium pyrophosphate dihydrate (CPP).

METHOD: We analysed 82 meniscal posterior horn samples (medial and lateral) collected from 41 human subjects. Among them, 20 individuals underwent total knee replacement due to medial compartment OA, while 21 deceased donors had no known knee OA. The assessment of meniscal calcifications and Pauli's histopathological scoring was conducted using histological sections. Furthermore, adjacent sections underwent measurement using Raman spectroscopy to characterize BCP and CPP calcifications based on their distinct spectral fingerprints.

RESULTS: All OA individuals exhibited calcifications in at least one meniscus, compared to 9.5% (95%CI 1%, 30%) of donors. Among 35 OA menisci with calcifications, 28(80%) had BCP, 5(14%) had CPP and 2(6%) had both types. In 4 donor menisci, 3(75%) had CPP while 1(25%) had both types. We estimated the association between Pauli score and presence of BCP in OA individuals, yielding an odds ratio of 2.1 (95%CI 0.8, 5.3) per 1 Pauli score. The association between Pauli score and presence of CPP (in whole study sample) seemed weaker, with odds ratio of 1.3 (95%CI 1.1, 1.7).

CONCLUSION: The presence of BCP was predominant in menisci of OA individuals, whereas CPP exhibited similar prevalence in individuals with and without OA. The formation of BCP crystals in menisci may represent an important and specific characteristic of OA disease process that warrants further attention.

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organization
publishing date
type
Contribution to journal
publication status
epub
subject
in
Osteoarthritis and Cartilage
publisher
Elsevier
external identifiers
  • pmid:39116991
ISSN
1063-4584
DOI
10.1016/j.joca.2024.07.013
language
English
LU publication?
yes
additional info
Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.
id
71eeb828-af9c-44ee-a4c0-a99390803e8c
date added to LUP
2024-08-11 19:11:14
date last changed
2024-08-12 09:06:21
@article{71eeb828-af9c-44ee-a4c0-a99390803e8c,
  abstract     = {{<p>OBJECTIVE: To investigate the occurrence of meniscal calcifications in individuals with and without knee osteoarthritis (OA). Additionally, we aim to identify the specific types of calcifications: basic calcium phosphate (BCP) and calcium pyrophosphate dihydrate (CPP).</p><p>METHOD: We analysed 82 meniscal posterior horn samples (medial and lateral) collected from 41 human subjects. Among them, 20 individuals underwent total knee replacement due to medial compartment OA, while 21 deceased donors had no known knee OA. The assessment of meniscal calcifications and Pauli's histopathological scoring was conducted using histological sections. Furthermore, adjacent sections underwent measurement using Raman spectroscopy to characterize BCP and CPP calcifications based on their distinct spectral fingerprints.</p><p>RESULTS: All OA individuals exhibited calcifications in at least one meniscus, compared to 9.5% (95%CI 1%, 30%) of donors. Among 35 OA menisci with calcifications, 28(80%) had BCP, 5(14%) had CPP and 2(6%) had both types. In 4 donor menisci, 3(75%) had CPP while 1(25%) had both types. We estimated the association between Pauli score and presence of BCP in OA individuals, yielding an odds ratio of 2.1 (95%CI 0.8, 5.3) per 1 Pauli score. The association between Pauli score and presence of CPP (in whole study sample) seemed weaker, with odds ratio of 1.3 (95%CI 1.1, 1.7).</p><p>CONCLUSION: The presence of BCP was predominant in menisci of OA individuals, whereas CPP exhibited similar prevalence in individuals with and without OA. The formation of BCP crystals in menisci may represent an important and specific characteristic of OA disease process that warrants further attention.</p>}},
  author       = {{Shakya, Bijay Ratna and Karjalainen, Ville-Pauli and Hellberg, Iida and Finnilä, Mikko A J and Elkhouly, Khaled and Sjögren, Amanda and Turkiewicz, Aleksandra and Önnerfjord, Patrik and Hughes, Velocity and Tjörnstrand, Jon and Englund, Martin and Saarakkala, Simo}},
  issn         = {{1063-4584}},
  language     = {{eng}},
  month        = {{08}},
  publisher    = {{Elsevier}},
  series       = {{Osteoarthritis and Cartilage}},
  title        = {{Prevalence and classification of meniscal calcifications in the human knee}},
  url          = {{http://dx.doi.org/10.1016/j.joca.2024.07.013}},
  doi          = {{10.1016/j.joca.2024.07.013}},
  year         = {{2024}},
}