Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

An early step in wobble uridine tRNA modification requires the Elongator complex

Huang, Bo ; Johansson, Marcus J O LU and Byström, Anders S (2005) In RNA 11(4). p.36-424
Abstract

Elongator has been reported to be a histone acetyltransferase complex involved in elongation of RNA polymerase II transcription. In Saccharomyces cerevisiae, mutations in any of the six Elongator protein subunit (ELP1-ELP6) genes or the three killer toxin insensitivity (KTI11-KTI13) genes cause similar pleiotropic phenotypes. By analyzing modified nucleosides in individual tRNA species, we show that the ELP1-ELP6 and KTI11-KTI13 genes are all required for an early step in synthesis of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) groups present on uridines at the wobble position in tRNA. Transfer RNA immunoprecipitation experiments showed that the Elp1 and Elp3 proteins specifically coprecipitate a tRNA susceptible to... (More)

Elongator has been reported to be a histone acetyltransferase complex involved in elongation of RNA polymerase II transcription. In Saccharomyces cerevisiae, mutations in any of the six Elongator protein subunit (ELP1-ELP6) genes or the three killer toxin insensitivity (KTI11-KTI13) genes cause similar pleiotropic phenotypes. By analyzing modified nucleosides in individual tRNA species, we show that the ELP1-ELP6 and KTI11-KTI13 genes are all required for an early step in synthesis of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) groups present on uridines at the wobble position in tRNA. Transfer RNA immunoprecipitation experiments showed that the Elp1 and Elp3 proteins specifically coprecipitate a tRNA susceptible to formation of an mcm5 side chain, indicating a direct role of Elongator in tRNA modification. The presence of mcm5U, ncm5U, or derivatives thereof at the wobble position is required for accurate and efficient translation, suggesting that the phenotypes of elp1-elp6 and kti11-kti13 mutants could be caused by a translational defect. Accordingly, a deletion of any ELP1-ELP6 or KTI11-KTI13 gene prevents an ochre suppressor tRNA that normally contains mcm5U from reading ochre stop codons.

(Less)
Please use this url to cite or link to this publication:
author
; and
publishing date
type
Contribution to journal
publication status
published
keywords
Acetyltransferases/genetics, Gene Deletion, Genes, Suppressor, Histone Acetyltransferases, Mutation, RNA, Transfer, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins/genetics, Schizosaccharomyces, Schizosaccharomyces pombe Proteins/genetics, Uridine/analogs & derivatives
in
RNA
volume
11
issue
4
pages
36 - 424
publisher
Cold Spring Harbor Laboratory Press (CSHL)
external identifiers
  • scopus:15444371415
  • pmid:15769872
ISSN
1355-8382
DOI
10.1261/rna.7247705
language
English
LU publication?
no
id
725bbdb6-0b7c-4528-9f95-1e0ad97c33ab
date added to LUP
2024-03-05 17:01:27
date last changed
2024-04-09 13:26:43
@article{725bbdb6-0b7c-4528-9f95-1e0ad97c33ab,
  abstract     = {{<p>Elongator has been reported to be a histone acetyltransferase complex involved in elongation of RNA polymerase II transcription. In Saccharomyces cerevisiae, mutations in any of the six Elongator protein subunit (ELP1-ELP6) genes or the three killer toxin insensitivity (KTI11-KTI13) genes cause similar pleiotropic phenotypes. By analyzing modified nucleosides in individual tRNA species, we show that the ELP1-ELP6 and KTI11-KTI13 genes are all required for an early step in synthesis of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) groups present on uridines at the wobble position in tRNA. Transfer RNA immunoprecipitation experiments showed that the Elp1 and Elp3 proteins specifically coprecipitate a tRNA susceptible to formation of an mcm5 side chain, indicating a direct role of Elongator in tRNA modification. The presence of mcm5U, ncm5U, or derivatives thereof at the wobble position is required for accurate and efficient translation, suggesting that the phenotypes of elp1-elp6 and kti11-kti13 mutants could be caused by a translational defect. Accordingly, a deletion of any ELP1-ELP6 or KTI11-KTI13 gene prevents an ochre suppressor tRNA that normally contains mcm5U from reading ochre stop codons.</p>}},
  author       = {{Huang, Bo and Johansson, Marcus J O and Byström, Anders S}},
  issn         = {{1355-8382}},
  keywords     = {{Acetyltransferases/genetics; Gene Deletion; Genes, Suppressor; Histone Acetyltransferases; Mutation; RNA, Transfer; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins/genetics; Schizosaccharomyces; Schizosaccharomyces pombe Proteins/genetics; Uridine/analogs & derivatives}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{36--424}},
  publisher    = {{Cold Spring Harbor Laboratory Press (CSHL)}},
  series       = {{RNA}},
  title        = {{An early step in wobble uridine tRNA modification requires the Elongator complex}},
  url          = {{http://dx.doi.org/10.1261/rna.7247705}},
  doi          = {{10.1261/rna.7247705}},
  volume       = {{11}},
  year         = {{2005}},
}