Protective effect of Zingerone against mouse testicular damage induced by zinc oxide nanoparticles
(2019) In Environmental Science and Pollution Research 26(25). p.25814-25824- Abstract
The purpose of the present study was to evaluate the effect of Zingerone (Zing) on zinc oxide nanoparticle (ZNP)-induced spermatogenesis defects in mice. To this end, 50 mg/kg of ZNP was prescribed to the mice as an intoxicated group for 35 days. In protection groups, Zing (10, 20, and 40 mg/kg) was given prior to ZNP treatment for seven days and then co-administration of ZNP for 35 days. Epididymal sperm parameters, testicular histology, Johnsen’s scoring, morphometric parameters, TUNEL staining, oxidative stress, and serum testosterone level were evaluated for determining ZNP and Zing effects on the mouse testicles. Effects of Zing and ZNP on the viability of mouse Leydig (TM3) and mouse Sertoli (TM4) cell lines were also done.... (More)
The purpose of the present study was to evaluate the effect of Zingerone (Zing) on zinc oxide nanoparticle (ZNP)-induced spermatogenesis defects in mice. To this end, 50 mg/kg of ZNP was prescribed to the mice as an intoxicated group for 35 days. In protection groups, Zing (10, 20, and 40 mg/kg) was given prior to ZNP treatment for seven days and then co-administration of ZNP for 35 days. Epididymal sperm parameters, testicular histology, Johnsen’s scoring, morphometric parameters, TUNEL staining, oxidative stress, and serum testosterone level were evaluated for determining ZNP and Zing effects on the mouse testicles. Effects of Zing and ZNP on the viability of mouse Leydig (TM3) and mouse Sertoli (TM4) cell lines were also done. Testicular weights, testosterone levels, sperm quality, morphometric parameters, Johnsen’s score, and superoxide dismutase (SOD) and catalase (CAT) activities were significantly decreased in ZNP-intoxicated mice, while apoptotic index, Malondialdehyde (MDA) content, and histological features, including epithelial vacuolization, sloughing, and germ cell detachment, were improved significantly in ZNP-intoxicated mice. Pretreatment with 20 or 40 mg/kg Zing significantly reduced the histological criteria, increased morphometric parameters, enhanced testosterone levels, attenuated apoptotic index, improved sperm quality, and reversed oxidative stress by reducing the level of MDA and incrementing the activity level of SOD and CAT enzymes. Zing dose-dependently enhanced the viability of ZNP-treated TM3 and TM4 cells in comparison with only ZNP-exposed cells. According to the results of our study, Zing effectively prevented the defects in spermatogenesis among mice treated by ZNP.
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- author
- Rafiee, Zeinab LU ; Khorsandi, Layasadat and Nejad-Dehbashi, Fereshteh
- publishing date
- 2019-09-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Apoptosis, Leydig cells, Oxidative stress, Sertoli cells, Zinc oxide nanoparticles, Zingerone
- in
- Environmental Science and Pollution Research
- volume
- 26
- issue
- 25
- pages
- 1 pages
- publisher
- Springer
- external identifiers
-
- scopus:85068789129
- pmid:31270769
- ISSN
- 0944-1344
- DOI
- 10.1007/s11356-019-05818-3
- language
- English
- LU publication?
- no
- additional info
- Funding Information: This paper was supported by a grant (96s79) from the student research committee council of the Ahvaz Jundishapur University of Medical Sciences. Publisher Copyright: © 2019, Springer-Verlag GmbH Germany, part of Springer Nature. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.
- id
- 72a71eee-ac2e-48bf-a8bd-8cc011add538
- date added to LUP
- 2021-09-23 20:37:37
- date last changed
- 2024-04-20 12:51:16
@article{72a71eee-ac2e-48bf-a8bd-8cc011add538,
abstract = {{<p>The purpose of the present study was to evaluate the effect of Zingerone (Zing) on zinc oxide nanoparticle (ZNP)-induced spermatogenesis defects in mice. To this end, 50 mg/kg of ZNP was prescribed to the mice as an intoxicated group for 35 days. In protection groups, Zing (10, 20, and 40 mg/kg) was given prior to ZNP treatment for seven days and then co-administration of ZNP for 35 days. Epididymal sperm parameters, testicular histology, Johnsen’s scoring, morphometric parameters, TUNEL staining, oxidative stress, and serum testosterone level were evaluated for determining ZNP and Zing effects on the mouse testicles. Effects of Zing and ZNP on the viability of mouse Leydig (TM3) and mouse Sertoli (TM4) cell lines were also done. Testicular weights, testosterone levels, sperm quality, morphometric parameters, Johnsen’s score, and superoxide dismutase (SOD) and catalase (CAT) activities were significantly decreased in ZNP-intoxicated mice, while apoptotic index, Malondialdehyde (MDA) content, and histological features, including epithelial vacuolization, sloughing, and germ cell detachment, were improved significantly in ZNP-intoxicated mice. Pretreatment with 20 or 40 mg/kg Zing significantly reduced the histological criteria, increased morphometric parameters, enhanced testosterone levels, attenuated apoptotic index, improved sperm quality, and reversed oxidative stress by reducing the level of MDA and incrementing the activity level of SOD and CAT enzymes. Zing dose-dependently enhanced the viability of ZNP-treated TM3 and TM4 cells in comparison with only ZNP-exposed cells. According to the results of our study, Zing effectively prevented the defects in spermatogenesis among mice treated by ZNP.</p>}},
author = {{Rafiee, Zeinab and Khorsandi, Layasadat and Nejad-Dehbashi, Fereshteh}},
issn = {{0944-1344}},
keywords = {{Apoptosis; Leydig cells; Oxidative stress; Sertoli cells; Zinc oxide nanoparticles; Zingerone}},
language = {{eng}},
month = {{09}},
number = {{25}},
pages = {{25814--25824}},
publisher = {{Springer}},
series = {{Environmental Science and Pollution Research}},
title = {{Protective effect of Zingerone against mouse testicular damage induced by zinc oxide nanoparticles}},
url = {{http://dx.doi.org/10.1007/s11356-019-05818-3}},
doi = {{10.1007/s11356-019-05818-3}},
volume = {{26}},
year = {{2019}},
}