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Blood Cells as a Cellular Biomarker for Mitochondrial Function in a Experimental Model of Acute Carbon Monoxide Poisoning with Treatment

Bungatavula, Devesh ; Greenwood, John C. ; Shofer, Frances S. ; Buehler, Guthrie ; Kao, Shih Han ; Kelly, Matthew ; Shin, Samuel S. ; Ehinger, Johannes K. LU orcid ; Kilbaugh, Todd J. and Jang, David H. (2025) In Journal of Medical Toxicology 21(3). p.327-335
Abstract

Introduction: Carbon monoxide (CO) is a leading cause of environmental poisoning in the United States with substantial mortality and morbidity. The mechanism of CO poisoning is complex and includes hypoxia, inflammation, and mitochondrial dysfunction. Currently both biomarkers and therapies for CO poisoning are limited and require new approaches. Methods: Rats (~ 300 g) were divided into four groups of ten rodents per group (exposure): Control (room air), CO-400 (400 ppm), CO-1000 (1000 ppm) and CO-2000 (2000 ppm). Rodents received the assigned exposure through a secured tracheotomy tube over 120 min followed by 30 min of re-oxygenation at room air for a total of 150 min. Five additional rodents in each group were administered a... (More)

Introduction: Carbon monoxide (CO) is a leading cause of environmental poisoning in the United States with substantial mortality and morbidity. The mechanism of CO poisoning is complex and includes hypoxia, inflammation, and mitochondrial dysfunction. Currently both biomarkers and therapies for CO poisoning are limited and require new approaches. Methods: Rats (~ 300 g) were divided into four groups of ten rodents per group (exposure): Control (room air), CO-400 (400 ppm), CO-1000 (1000 ppm) and CO-2000 (2000 ppm). Rodents received the assigned exposure through a secured tracheotomy tube over 120 min followed by 30 min of re-oxygenation at room air for a total of 150 min. Five additional rodents in each group were administered a succinate prodrug (NV354) at the start of exposure for the duration of the experiment until the reoxygenation period as separate experiments. Cortical brain tissue and whole blood were obtained for mitochondrial respiration. Stored plasma and snap frozen tissue stored at -80oC were used to obtain protein quantification with Western Blotting. Results: All animals in the Sham, CO-400, and CO-1000 groups survived until the end of the exposure period; no animals in the CO-2000 groups survived the exposure and were counted as attrition. We observed a dose-dependent decrease in key respiratory states in both isolated brain mitochondria and peripheral blood mononuclear cells (PBMCs), and, PBMCs respiration more positively correlated with isolated brain mitochondria when compared to carboxyhemoglobin (COHb). There was no significant difference in mitochondrial respiratory states in animals treated with NV354 compared to the untreated group. Conclusions: The primary findings from this study include: (1) A dose-dependent decrease with key respiration states with higher concentrations of CO; (2) PBMCs had a higher correlation to isolated brain mitochondria respiration when compared to COHb; and (3) there was no treatment effect with the use of NV354.

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author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Basic science, Biomarker, Blood Cells, Carbon Monoxide, Mitochondria
in
Journal of Medical Toxicology
volume
21
issue
3
pages
9 pages
publisher
Springer
external identifiers
  • pmid:40295447
  • scopus:105003751732
ISSN
1556-9039
DOI
10.1007/s13181-025-01077-6
language
English
LU publication?
yes
id
72d1a610-94a5-41eb-8212-c659aadb5884
date added to LUP
2025-09-22 13:40:09
date last changed
2025-09-23 03:00:03
@article{72d1a610-94a5-41eb-8212-c659aadb5884,
  abstract     = {{<p>Introduction: Carbon monoxide (CO) is a leading cause of environmental poisoning in the United States with substantial mortality and morbidity. The mechanism of CO poisoning is complex and includes hypoxia, inflammation, and mitochondrial dysfunction. Currently both biomarkers and therapies for CO poisoning are limited and require new approaches. Methods: Rats (~ 300 g) were divided into four groups of ten rodents per group (exposure): Control (room air), CO-400 (400 ppm), CO-1000 (1000 ppm) and CO-2000 (2000 ppm). Rodents received the assigned exposure through a secured tracheotomy tube over 120 min followed by 30 min of re-oxygenation at room air for a total of 150 min. Five additional rodents in each group were administered a succinate prodrug (NV354) at the start of exposure for the duration of the experiment until the reoxygenation period as separate experiments. Cortical brain tissue and whole blood were obtained for mitochondrial respiration. Stored plasma and snap frozen tissue stored at -80<sup>o</sup>C were used to obtain protein quantification with Western Blotting. Results: All animals in the Sham, CO-400, and CO-1000 groups survived until the end of the exposure period; no animals in the CO-2000 groups survived the exposure and were counted as attrition. We observed a dose-dependent decrease in key respiratory states in both isolated brain mitochondria and peripheral blood mononuclear cells (PBMCs), and, PBMCs respiration more positively correlated with isolated brain mitochondria when compared to carboxyhemoglobin (COHb). There was no significant difference in mitochondrial respiratory states in animals treated with NV354 compared to the untreated group. Conclusions: The primary findings from this study include: (1) A dose-dependent decrease with key respiration states with higher concentrations of CO; (2) PBMCs had a higher correlation to isolated brain mitochondria respiration when compared to COHb; and (3) there was no treatment effect with the use of NV354.</p>}},
  author       = {{Bungatavula, Devesh and Greenwood, John C. and Shofer, Frances S. and Buehler, Guthrie and Kao, Shih Han and Kelly, Matthew and Shin, Samuel S. and Ehinger, Johannes K. and Kilbaugh, Todd J. and Jang, David H.}},
  issn         = {{1556-9039}},
  keywords     = {{Basic science; Biomarker; Blood Cells; Carbon Monoxide; Mitochondria}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{327--335}},
  publisher    = {{Springer}},
  series       = {{Journal of Medical Toxicology}},
  title        = {{Blood Cells as a Cellular Biomarker for Mitochondrial Function in a Experimental Model of Acute Carbon Monoxide Poisoning with Treatment}},
  url          = {{http://dx.doi.org/10.1007/s13181-025-01077-6}},
  doi          = {{10.1007/s13181-025-01077-6}},
  volume       = {{21}},
  year         = {{2025}},
}