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Direct Electron Transfer of Cellobiose Dehydrogenase on Positively Charged Polyethyleneimine Gold Nanoparticles

Tavahodi, Mojtaba; Ortiz, Roberto LU ; Schulz, Christopher LU ; Ekhtiari, Ali; Ludwig, Roland; Haghighi, Behzad LU and Gorton, Lo LU (2017) In ChemPlusChem 82(4). p.546-552
Abstract

Efficient conjugation between biomolecules and electrode materials is one of the main challenges in the field of biosensors. Cellobiose dehydrogenase (CDH) is a monomeric enzyme, which consists of two separate domains: one catalytic dehydrogenase domain (DHCDH) carrying strongly bound flavin adenine dinucleotide (FAD) in the active site and a cytochrome domain (CYTCDH) carrying a b-type heme connected by a flexible linker region. Herein, we report on the development of a lactose biosensor, based on direct electron transfer (DET) from CDH from Phanerochaete sordida (PsCDH) electrostatically attached onto polyethyleneimine-stabilized gold nanoparticles (PEI@AuNPs) used to cover a conventional polycrystalline solid... (More)

Efficient conjugation between biomolecules and electrode materials is one of the main challenges in the field of biosensors. Cellobiose dehydrogenase (CDH) is a monomeric enzyme, which consists of two separate domains: one catalytic dehydrogenase domain (DHCDH) carrying strongly bound flavin adenine dinucleotide (FAD) in the active site and a cytochrome domain (CYTCDH) carrying a b-type heme connected by a flexible linker region. Herein, we report on the development of a lactose biosensor, based on direct electron transfer (DET) from CDH from Phanerochaete sordida (PsCDH) electrostatically attached onto polyethyleneimine-stabilized gold nanoparticles (PEI@AuNPs) used to cover a conventional polycrystalline solid gold disk electrode. PEI@AuNPs were synthesized in aqueous solution using PEI as reducing agent for AuIII and as stabilizer for the nanoparticles. The heterogeneous electron-transfer (ET) rate (ks) for the redox reaction of immobilized PsCDH at the modified electrodes was calculated based on the Laviron theory and was found to be (39.6±2.5)s-1. The proposed lactose biosensor exhibits good long term stability as well as high and reproducible sensitivity to lactose with a response time less than 5s and a linear range from 1 to 100μm.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Biosensors, Electron transfer, Enzymes, Gold, Nanoparticles
in
ChemPlusChem
volume
82
issue
4
pages
546 - 552
publisher
Institute of Organic Chemistry and Biochemistry
external identifiers
  • scopus:84996946589
  • wos:000400603500005
ISSN
2192-6506
DOI
10.1002/cplu.201600453
language
English
LU publication?
yes
id
73157b76-6764-4bc5-ad97-9a7bff5181f2
date added to LUP
2017-02-21 11:50:02
date last changed
2018-05-06 04:31:09
@article{73157b76-6764-4bc5-ad97-9a7bff5181f2,
  abstract     = {<p>Efficient conjugation between biomolecules and electrode materials is one of the main challenges in the field of biosensors. Cellobiose dehydrogenase (CDH) is a monomeric enzyme, which consists of two separate domains: one catalytic dehydrogenase domain (DH<sub>CDH</sub>) carrying strongly bound flavin adenine dinucleotide (FAD) in the active site and a cytochrome domain (CYT<sub>CDH</sub>) carrying a b-type heme connected by a flexible linker region. Herein, we report on the development of a lactose biosensor, based on direct electron transfer (DET) from CDH from Phanerochaete sordida (PsCDH) electrostatically attached onto polyethyleneimine-stabilized gold nanoparticles (PEI@AuNPs) used to cover a conventional polycrystalline solid gold disk electrode. PEI@AuNPs were synthesized in aqueous solution using PEI as reducing agent for Au<sup>III</sup> and as stabilizer for the nanoparticles. The heterogeneous electron-transfer (ET) rate (k<sub>s</sub>) for the redox reaction of immobilized PsCDH at the modified electrodes was calculated based on the Laviron theory and was found to be (39.6±2.5)s<sup>-1</sup>. The proposed lactose biosensor exhibits good long term stability as well as high and reproducible sensitivity to lactose with a response time less than 5s and a linear range from 1 to 100μm.</p>},
  author       = {Tavahodi, Mojtaba and Ortiz, Roberto and Schulz, Christopher and Ekhtiari, Ali and Ludwig, Roland and Haghighi, Behzad and Gorton, Lo},
  issn         = {2192-6506},
  keyword      = {Biosensors,Electron transfer,Enzymes,Gold,Nanoparticles},
  language     = {eng},
  number       = {4},
  pages        = {546--552},
  publisher    = {Institute of Organic Chemistry and Biochemistry},
  series       = {ChemPlusChem},
  title        = {Direct Electron Transfer of Cellobiose Dehydrogenase on Positively Charged Polyethyleneimine Gold Nanoparticles},
  url          = {http://dx.doi.org/10.1002/cplu.201600453},
  volume       = {82},
  year         = {2017},
}