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Mannan-binding lectin in women with a history of recurrent vulvovaginal candidiasis.

Henic, Emir LU ; Thiel, Steffen and Mårdh, Per-Anders LU (2010) In European Journal of Obstetrics, Gynecology, and Reproductive Biology 148(2). p.163-165
Abstract
OBJECTIVES: To determine the serum concentration of mannan-binding lectin (MBL), a component of the innate immune system, in women with a history of recurrent vulvovaginal candidiasis (RVVC) and to correlate the result to candida-cultures, contraceptive use, if any, and to different antifungal therapies. STUDY DESIGN: Twenty-nine women with a history of RVVC were investigated. Cultures of vulvar and vaginal samples were grown on chromogenic agar. Serum levels of MBL were determined by a sandwich time-resolved immunofluorometric assay, using anti-MBL coated microtiter wells containing samples, which were washed, incubated with biotinylated anti-MBL followed by europium-labeled streptavidin and measured by time-resolved flourometry. RESULTS:... (More)
OBJECTIVES: To determine the serum concentration of mannan-binding lectin (MBL), a component of the innate immune system, in women with a history of recurrent vulvovaginal candidiasis (RVVC) and to correlate the result to candida-cultures, contraceptive use, if any, and to different antifungal therapies. STUDY DESIGN: Twenty-nine women with a history of RVVC were investigated. Cultures of vulvar and vaginal samples were grown on chromogenic agar. Serum levels of MBL were determined by a sandwich time-resolved immunofluorometric assay, using anti-MBL coated microtiter wells containing samples, which were washed, incubated with biotinylated anti-MBL followed by europium-labeled streptavidin and measured by time-resolved flourometry. RESULTS: The median MBL level was higher in the RVVC cases than in 30 women with no history of genital candida infection who served as a comparison group (p=0.006). It was also higher in the candida-positive than in the culture-negative RVVC (p=0.02). The median concentration of MBL was also higher in hormonal contraceptive users as compared to condom-users and those using no contraceptive at all (p=0.03). CONCLUSION: The result indicates a role of MBL in RVVC and the production may correlate to vulvar/vaginal colonization by Candida, hormonal contraceptive use, and antifungal therapies. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
European Journal of Obstetrics, Gynecology, and Reproductive Biology
volume
148
issue
2
pages
163 - 165
publisher
Elsevier
external identifiers
  • wos:000274845400013
  • pmid:19910100
  • scopus:73749084681
ISSN
0301-2115
DOI
10.1016/j.ejogrb.2009.10.008
language
English
LU publication?
yes
id
73377111-cb2c-4e3a-a4e0-a9ca5305c975 (old id 1511977)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19910100?dopt=Abstract
date added to LUP
2009-12-01 15:31:51
date last changed
2018-01-07 04:18:09
@article{73377111-cb2c-4e3a-a4e0-a9ca5305c975,
  abstract     = {OBJECTIVES: To determine the serum concentration of mannan-binding lectin (MBL), a component of the innate immune system, in women with a history of recurrent vulvovaginal candidiasis (RVVC) and to correlate the result to candida-cultures, contraceptive use, if any, and to different antifungal therapies. STUDY DESIGN: Twenty-nine women with a history of RVVC were investigated. Cultures of vulvar and vaginal samples were grown on chromogenic agar. Serum levels of MBL were determined by a sandwich time-resolved immunofluorometric assay, using anti-MBL coated microtiter wells containing samples, which were washed, incubated with biotinylated anti-MBL followed by europium-labeled streptavidin and measured by time-resolved flourometry. RESULTS: The median MBL level was higher in the RVVC cases than in 30 women with no history of genital candida infection who served as a comparison group (p=0.006). It was also higher in the candida-positive than in the culture-negative RVVC (p=0.02). The median concentration of MBL was also higher in hormonal contraceptive users as compared to condom-users and those using no contraceptive at all (p=0.03). CONCLUSION: The result indicates a role of MBL in RVVC and the production may correlate to vulvar/vaginal colonization by Candida, hormonal contraceptive use, and antifungal therapies.},
  author       = {Henic, Emir and Thiel, Steffen and Mårdh, Per-Anders},
  issn         = {0301-2115},
  language     = {eng},
  number       = {2},
  pages        = {163--165},
  publisher    = {Elsevier},
  series       = {European Journal of Obstetrics, Gynecology, and Reproductive Biology},
  title        = {Mannan-binding lectin in women with a history of recurrent vulvovaginal candidiasis.},
  url          = {http://dx.doi.org/10.1016/j.ejogrb.2009.10.008},
  volume       = {148},
  year         = {2010},
}