Joining chain-expressing and -nonexpressing B cell populations in the mouse
(2001) In Journal of Experimental Medicine 194(5). p.557-570- Abstract
- The diphtheria toxin A chain (DTA) was gene targeted into the Joining chain (J chain) locus to create a mouse strain selecting against J chain-expressing cells, JDTA mice. Serum immunoglobulin (Ig)M and serum IgG were reduced six to eightfold, while serum IgA was elevated 14-fold in these mice. JDTA mice were immune competent although the serum Ig response compared with wild-type mice was reduced sixfold at day 14 but only fourfold at day 45 after immunization. Exchanging the DTA gene with a cDNA for c-myc resulted in mice with a distinct phenotype with increased Ig production and enhanced humoral immune responses. Analysis of single B cells stimulated by lipopolysaccharide in vitro using reverse transcription-polymerase chain reaction... (More)
- The diphtheria toxin A chain (DTA) was gene targeted into the Joining chain (J chain) locus to create a mouse strain selecting against J chain-expressing cells, JDTA mice. Serum immunoglobulin (Ig)M and serum IgG were reduced six to eightfold, while serum IgA was elevated 14-fold in these mice. JDTA mice were immune competent although the serum Ig response compared with wild-type mice was reduced sixfold at day 14 but only fourfold at day 45 after immunization. Exchanging the DTA gene with a cDNA for c-myc resulted in mice with a distinct phenotype with increased Ig production and enhanced humoral immune responses. Analysis of single B cells stimulated by lipopolysaccharide in vitro using reverse transcription-polymerase chain reaction showed that J chain-nonexpressing B cells could be detected that had a secretory phenotype as determined by an abundance of transcript for secretory IgM. Finally, limiting dilution analysis of peripheral B cells showed that J chain expression was a clonal property already established in naive, peripheral B lymphocytes. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1121591
- author
- Erlandsson, Lena ; Akerblad, Peter ; Vingsbo-Lundberg, C ; Källberg, Eva LU ; Lycke, Nils and Leanderson, Tomas
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- B cells, plasma cells, mice, ES cells, J chain
- in
- Journal of Experimental Medicine
- volume
- 194
- issue
- 5
- pages
- 557 - 570
- publisher
- Rockefeller University Press
- external identifiers
-
- pmid:11535625
- scopus:0035801695
- ISSN
- 1540-9538
- DOI
- 10.1084/jem.194.5.557
- language
- English
- LU publication?
- yes
- id
- 735523de-b7a2-4810-865b-96eeb3173d40 (old id 1121591)
- date added to LUP
- 2016-04-01 16:48:49
- date last changed
- 2022-04-23 00:44:16
@article{735523de-b7a2-4810-865b-96eeb3173d40, abstract = {{The diphtheria toxin A chain (DTA) was gene targeted into the Joining chain (J chain) locus to create a mouse strain selecting against J chain-expressing cells, JDTA mice. Serum immunoglobulin (Ig)M and serum IgG were reduced six to eightfold, while serum IgA was elevated 14-fold in these mice. JDTA mice were immune competent although the serum Ig response compared with wild-type mice was reduced sixfold at day 14 but only fourfold at day 45 after immunization. Exchanging the DTA gene with a cDNA for c-myc resulted in mice with a distinct phenotype with increased Ig production and enhanced humoral immune responses. Analysis of single B cells stimulated by lipopolysaccharide in vitro using reverse transcription-polymerase chain reaction showed that J chain-nonexpressing B cells could be detected that had a secretory phenotype as determined by an abundance of transcript for secretory IgM. Finally, limiting dilution analysis of peripheral B cells showed that J chain expression was a clonal property already established in naive, peripheral B lymphocytes.}}, author = {{Erlandsson, Lena and Akerblad, Peter and Vingsbo-Lundberg, C and Källberg, Eva and Lycke, Nils and Leanderson, Tomas}}, issn = {{1540-9538}}, keywords = {{B cells; plasma cells; mice; ES cells; J chain}}, language = {{eng}}, number = {{5}}, pages = {{557--570}}, publisher = {{Rockefeller University Press}}, series = {{Journal of Experimental Medicine}}, title = {{Joining chain-expressing and -nonexpressing B cell populations in the mouse}}, url = {{http://dx.doi.org/10.1084/jem.194.5.557}}, doi = {{10.1084/jem.194.5.557}}, volume = {{194}}, year = {{2001}}, }