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Joining chain-expressing and -nonexpressing B cell populations in the mouse

Erlandsson, Lena ; Akerblad, Peter ; Vingsbo-Lundberg, C ; Källberg, Eva LU ; Lycke, Nils and Leanderson, Tomas (2001) In Journal of Experimental Medicine 194(5). p.557-570
Abstract
The diphtheria toxin A chain (DTA) was gene targeted into the Joining chain (J chain) locus to create a mouse strain selecting against J chain-expressing cells, JDTA mice. Serum immunoglobulin (Ig)M and serum IgG were reduced six to eightfold, while serum IgA was elevated 14-fold in these mice. JDTA mice were immune competent although the serum Ig response compared with wild-type mice was reduced sixfold at day 14 but only fourfold at day 45 after immunization. Exchanging the DTA gene with a cDNA for c-myc resulted in mice with a distinct phenotype with increased Ig production and enhanced humoral immune responses. Analysis of single B cells stimulated by lipopolysaccharide in vitro using reverse transcription-polymerase chain reaction... (More)
The diphtheria toxin A chain (DTA) was gene targeted into the Joining chain (J chain) locus to create a mouse strain selecting against J chain-expressing cells, JDTA mice. Serum immunoglobulin (Ig)M and serum IgG were reduced six to eightfold, while serum IgA was elevated 14-fold in these mice. JDTA mice were immune competent although the serum Ig response compared with wild-type mice was reduced sixfold at day 14 but only fourfold at day 45 after immunization. Exchanging the DTA gene with a cDNA for c-myc resulted in mice with a distinct phenotype with increased Ig production and enhanced humoral immune responses. Analysis of single B cells stimulated by lipopolysaccharide in vitro using reverse transcription-polymerase chain reaction showed that J chain-nonexpressing B cells could be detected that had a secretory phenotype as determined by an abundance of transcript for secretory IgM. Finally, limiting dilution analysis of peripheral B cells showed that J chain expression was a clonal property already established in naive, peripheral B lymphocytes. (Less)
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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
B cells, plasma cells, mice, ES cells, J chain
in
Journal of Experimental Medicine
volume
194
issue
5
pages
557 - 570
publisher
Rockefeller University Press
external identifiers
  • pmid:11535625
  • scopus:0035801695
ISSN
1540-9538
DOI
10.1084/jem.194.5.557
language
English
LU publication?
yes
id
735523de-b7a2-4810-865b-96eeb3173d40 (old id 1121591)
date added to LUP
2016-04-01 16:48:49
date last changed
2025-10-14 11:07:07
@article{735523de-b7a2-4810-865b-96eeb3173d40,
  abstract     = {{The diphtheria toxin A chain (DTA) was gene targeted into the Joining chain (J chain) locus to create a mouse strain selecting against J chain-expressing cells, JDTA mice. Serum immunoglobulin (Ig)M and serum IgG were reduced six to eightfold, while serum IgA was elevated 14-fold in these mice. JDTA mice were immune competent although the serum Ig response compared with wild-type mice was reduced sixfold at day 14 but only fourfold at day 45 after immunization. Exchanging the DTA gene with a cDNA for c-myc resulted in mice with a distinct phenotype with increased Ig production and enhanced humoral immune responses. Analysis of single B cells stimulated by lipopolysaccharide in vitro using reverse transcription-polymerase chain reaction showed that J chain-nonexpressing B cells could be detected that had a secretory phenotype as determined by an abundance of transcript for secretory IgM. Finally, limiting dilution analysis of peripheral B cells showed that J chain expression was a clonal property already established in naive, peripheral B lymphocytes.}},
  author       = {{Erlandsson, Lena and Akerblad, Peter and Vingsbo-Lundberg, C and Källberg, Eva and Lycke, Nils and Leanderson, Tomas}},
  issn         = {{1540-9538}},
  keywords     = {{B cells; plasma cells; mice; ES cells; J chain}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{557--570}},
  publisher    = {{Rockefeller University Press}},
  series       = {{Journal of Experimental Medicine}},
  title        = {{Joining chain-expressing and -nonexpressing B cell populations in the mouse}},
  url          = {{http://dx.doi.org/10.1084/jem.194.5.557}},
  doi          = {{10.1084/jem.194.5.557}},
  volume       = {{194}},
  year         = {{2001}},
}