Impact of xylose epimerase on sugar assimilation and sensing in recombinant Saccharomyces cerevisiae carrying different xylose-utilization pathways
(2023) In Biotechnology for Biofuels and Bioproducts 16(1).- Abstract
- Background
Over the last decades, many strategies to procure and improve xylose consumption in Saccharomyces cerevisiae have been reported. This includes the introduction of efficient xylose-assimilating enzymes, the improvement of xylose transport, or the alteration of the sugar signaling response. However, different strain backgrounds are often used, making it difficult to determine if the findings are transferrable both to other xylose-consuming strains and to other xylose-assimilation pathways. For example, the influence of anomerization rates between α- and β-xylopyranose in pathway optimization and sugar sensing is relatively unexplored.
Results
In this study, we tested the effect of expressing a xylose epimerase in... (More) - Background
Over the last decades, many strategies to procure and improve xylose consumption in Saccharomyces cerevisiae have been reported. This includes the introduction of efficient xylose-assimilating enzymes, the improvement of xylose transport, or the alteration of the sugar signaling response. However, different strain backgrounds are often used, making it difficult to determine if the findings are transferrable both to other xylose-consuming strains and to other xylose-assimilation pathways. For example, the influence of anomerization rates between α- and β-xylopyranose in pathway optimization and sugar sensing is relatively unexplored.
Results
In this study, we tested the effect of expressing a xylose epimerase in S. cerevisiae strains carrying different xylose-consuming routes. First, XIs originating from three different species in isogenic S. cerevisiae strains were tested and the XI from Lachnoclostridium phytofermentans was found to give the best performance. The benefit of increasing the anomerization rate of xylose by adding a xylose epimerase to the XI strains was confirmed, as higher biomass formation and faster xylose consumption were obtained. However, the impact of xylose epimerase was XI-dependent, indicating that anomer preference may differ from enzyme to enzyme. The addition of the xylose epimerase in xylose reductase/xylitol dehydrogenase (XR/XDH)-carrying strains gave no improvement in xylose assimilation, suggesting that the XR from Spathaspora passalidarum had no anomer preference, in contrast to other reported XRs. The reduction in accumulated xylitol that was observed when the xylose epimerase was added may be associated with the upregulation of genes encoding endogenous aldose reductases which could be affected by the anomerization rate. Finally, xylose epimerase addition did not affect the sugar signaling, whereas the type of xylose pathway (XI vs. XR/XDH) did.
Conclusions
Although xylose anomer specificity is often overlooked, the addition of xylose epimerase should be considered as a key engineering step, especially when using the best-performing XI enzyme from L. phytofermentans. Additional research into the binding mechanism of xylose is needed to elucidate the enzyme-specific effect and decrease in xylitol accumulation. Finally, the differences in sugar signaling responses between XI and XR/XDH strains indicate that either the redox balance or the growth rate impacts the SNF1/Mig1p sensing pathway. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/735760b3-7660-4407-a790-9a9aae187e88
- author
- Persson, Viktor C. LU ; Perruca Foncillas, Raquel LU ; Anderes, Tegan R. ; Ginestet, Clément and Gorwa-Grauslund, Marie F LU
- organization
- publishing date
- 2023-11-06
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- xylose epimerase, xylopyranose, Sugar signaling, biosensor, xylose isomerase, xylose reductase, aldose-1-epimerase, epimerase, anomers, redox, saccharomyces, cerevisiae, snf1, SNF1/Mig1p
- in
- Biotechnology for Biofuels and Bioproducts
- volume
- 16
- issue
- 1
- article number
- 168
- publisher
- BioMed Central (BMC)
- external identifiers
-
- scopus:85175829605
- pmid:37932829
- ISSN
- 2731-3654
- DOI
- 10.1186/s13068-023-02422-z
- project
- Understanding, mapping and engineering xylose signaling in industrially relevant yeast species
- On line monitoring of yeast population fitness to improve energy-related fermentation processes
- language
- English
- LU publication?
- yes
- id
- 735760b3-7660-4407-a790-9a9aae187e88
- date added to LUP
- 2023-11-17 12:54:45
- date last changed
- 2024-02-17 03:00:47
@article{735760b3-7660-4407-a790-9a9aae187e88,
abstract = {{Background<br/>Over the last decades, many strategies to procure and improve xylose consumption in Saccharomyces cerevisiae have been reported. This includes the introduction of efficient xylose-assimilating enzymes, the improvement of xylose transport, or the alteration of the sugar signaling response. However, different strain backgrounds are often used, making it difficult to determine if the findings are transferrable both to other xylose-consuming strains and to other xylose-assimilation pathways. For example, the influence of anomerization rates between α- and β-xylopyranose in pathway optimization and sugar sensing is relatively unexplored.<br/><br/>Results<br/>In this study, we tested the effect of expressing a xylose epimerase in S. cerevisiae strains carrying different xylose-consuming routes. First, XIs originating from three different species in isogenic S. cerevisiae strains were tested and the XI from Lachnoclostridium phytofermentans was found to give the best performance. The benefit of increasing the anomerization rate of xylose by adding a xylose epimerase to the XI strains was confirmed, as higher biomass formation and faster xylose consumption were obtained. However, the impact of xylose epimerase was XI-dependent, indicating that anomer preference may differ from enzyme to enzyme. The addition of the xylose epimerase in xylose reductase/xylitol dehydrogenase (XR/XDH)-carrying strains gave no improvement in xylose assimilation, suggesting that the XR from Spathaspora passalidarum had no anomer preference, in contrast to other reported XRs. The reduction in accumulated xylitol that was observed when the xylose epimerase was added may be associated with the upregulation of genes encoding endogenous aldose reductases which could be affected by the anomerization rate. Finally, xylose epimerase addition did not affect the sugar signaling, whereas the type of xylose pathway (XI vs. XR/XDH) did.<br/><br/>Conclusions<br/>Although xylose anomer specificity is often overlooked, the addition of xylose epimerase should be considered as a key engineering step, especially when using the best-performing XI enzyme from L. phytofermentans. Additional research into the binding mechanism of xylose is needed to elucidate the enzyme-specific effect and decrease in xylitol accumulation. Finally, the differences in sugar signaling responses between XI and XR/XDH strains indicate that either the redox balance or the growth rate impacts the SNF1/Mig1p sensing pathway.}},
author = {{Persson, Viktor C. and Perruca Foncillas, Raquel and Anderes, Tegan R. and Ginestet, Clément and Gorwa-Grauslund, Marie F}},
issn = {{2731-3654}},
keywords = {{xylose epimerase; xylopyranose; Sugar signaling; biosensor; xylose isomerase; xylose reductase; aldose-1-epimerase; epimerase; anomers; redox; saccharomyces; cerevisiae; snf1; SNF1/Mig1p}},
language = {{eng}},
month = {{11}},
number = {{1}},
publisher = {{BioMed Central (BMC)}},
series = {{Biotechnology for Biofuels and Bioproducts}},
title = {{Impact of xylose epimerase on sugar assimilation and sensing in recombinant Saccharomyces cerevisiae carrying different xylose-utilization pathways}},
url = {{http://dx.doi.org/10.1186/s13068-023-02422-z}},
doi = {{10.1186/s13068-023-02422-z}},
volume = {{16}},
year = {{2023}},
}