Isolation, characterization and multiplex polymerase chain reaction of novel microsatellite loci for the avian parasite Philornis downsi (Diptera: Muscidae)
(2008) In Molecular Ecology Resources 8(1). p.142-144- Abstract
- An enrichment technique was used to isolate 11 di-, tri-, and tetra microsatellites for the parasitic fly Philornis downsi (Diptera: Muscidae). These loci were polymerase chain reaction amplified in singleplexes or two-plexes for P. downsi. The loci showed low to moderate polymorphism, exhibited between three and four alleles, and observed heterozygosity ranged from 0.05 to 0.86. These new markers will be useful for population-level and paternity analyses and will provide valuable information about the ecology of this high-impact parasite of vulnerable bird species.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3738422
- author
- Dudaniec, Rachael LU ; Gardner, Michael G. and Kleindorfer, Sonia
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Darwin's finches, ectoparasitism, microsatellites, multiplex PCR, paternity, Philornis
- in
- Molecular Ecology Resources
- volume
- 8
- issue
- 1
- pages
- 142 - 144
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:36849030765
- ISSN
- 1755-098X
- DOI
- 10.1111/j.1471-8286.2007.01900.x
- language
- English
- LU publication?
- no
- additional info
- 1
- id
- 73907bd5-7eb9-4e0c-889a-4bfbad3baec2 (old id 3738422)
- date added to LUP
- 2016-04-01 12:33:23
- date last changed
- 2022-01-27 06:44:02
@article{73907bd5-7eb9-4e0c-889a-4bfbad3baec2, abstract = {{An enrichment technique was used to isolate 11 di-, tri-, and tetra microsatellites for the parasitic fly Philornis downsi (Diptera: Muscidae). These loci were polymerase chain reaction amplified in singleplexes or two-plexes for P. downsi. The loci showed low to moderate polymorphism, exhibited between three and four alleles, and observed heterozygosity ranged from 0.05 to 0.86. These new markers will be useful for population-level and paternity analyses and will provide valuable information about the ecology of this high-impact parasite of vulnerable bird species.}}, author = {{Dudaniec, Rachael and Gardner, Michael G. and Kleindorfer, Sonia}}, issn = {{1755-098X}}, keywords = {{Darwin's finches; ectoparasitism; microsatellites; multiplex PCR; paternity; Philornis}}, language = {{eng}}, number = {{1}}, pages = {{142--144}}, publisher = {{Wiley-Blackwell}}, series = {{Molecular Ecology Resources}}, title = {{Isolation, characterization and multiplex polymerase chain reaction of novel microsatellite loci for the avian parasite Philornis downsi (Diptera: Muscidae)}}, url = {{http://dx.doi.org/10.1111/j.1471-8286.2007.01900.x}}, doi = {{10.1111/j.1471-8286.2007.01900.x}}, volume = {{8}}, year = {{2008}}, }