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Separation of 100-kilobase DNA molecules in 10 seconds

Bakajin, O. ; Duke, T. A J ; Tegenfeldt, J. LU orcid ; Chou, C. F. ; Chan, S. S. LU ; Austin, R. H. and Cox, E. C. (2001) In Analytical Chemistry 73(24). p.6053-6056
Abstract

Long double-stranded DNA molecules were separated in microfabricated hexagonal arrays in less than 1 min, several orders of magnitude faster than by using conventional technology. DNA samples were first concentrated at the entrance to the array in a thin band by entropic focusing. They were then separated by pulsed field electrophoresis. T4 (168.9 kbp) and λ (48.5 kbp) DNAs could be resolved into two clearly separated bands in ∼10 s in these experiments. This corresponds to a mass resolution of 6% in 11 min in a 1-cm-long array.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
in
Analytical Chemistry
volume
73
issue
24
pages
4 pages
publisher
The American Chemical Society (ACS)
external identifiers
  • pmid:11791579
  • scopus:0035894044
ISSN
0003-2700
DOI
10.1021/ac015527o
language
English
LU publication?
yes
id
73a008d4-1f93-434f-8fbe-3c79b138adb8
date added to LUP
2018-10-20 10:42:23
date last changed
2025-01-22 21:39:53
@article{73a008d4-1f93-434f-8fbe-3c79b138adb8,
  abstract     = {{<p>Long double-stranded DNA molecules were separated in microfabricated hexagonal arrays in less than 1 min, several orders of magnitude faster than by using conventional technology. DNA samples were first concentrated at the entrance to the array in a thin band by entropic focusing. They were then separated by pulsed field electrophoresis. T4 (168.9 kbp) and λ (48.5 kbp) DNAs could be resolved into two clearly separated bands in ∼10 s in these experiments. This corresponds to a mass resolution of 6% in 11 min in a 1-cm-long array.</p>}},
  author       = {{Bakajin, O. and Duke, T. A J and Tegenfeldt, J. and Chou, C. F. and Chan, S. S. and Austin, R. H. and Cox, E. C.}},
  issn         = {{0003-2700}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{24}},
  pages        = {{6053--6056}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Analytical Chemistry}},
  title        = {{Separation of 100-kilobase DNA molecules in 10 seconds}},
  url          = {{http://dx.doi.org/10.1021/ac015527o}},
  doi          = {{10.1021/ac015527o}},
  volume       = {{73}},
  year         = {{2001}},
}