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Separation of 100-kilobase DNA molecules in 10 seconds

Bakajin, O. ; Duke, T. A J ; Tegenfeldt, J. LU ; Chou, C. F. ; Chan, S. S. LU ; Austin, R. H. and Cox, E. C. (2001) In Analytical Chemistry 73(24). p.6053-6056
Abstract

Long double-stranded DNA molecules were separated in microfabricated hexagonal arrays in less than 1 min, several orders of magnitude faster than by using conventional technology. DNA samples were first concentrated at the entrance to the array in a thin band by entropic focusing. They were then separated by pulsed field electrophoresis. T4 (168.9 kbp) and λ (48.5 kbp) DNAs could be resolved into two clearly separated bands in ∼10 s in these experiments. This corresponds to a mass resolution of 6% in 11 min in a 1-cm-long array.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
in
Analytical Chemistry
volume
73
issue
24
pages
4 pages
publisher
The American Chemical Society (ACS)
external identifiers
  • scopus:0035894044
  • pmid:11791579
ISSN
0003-2700
DOI
10.1021/ac015527o
language
English
LU publication?
yes
id
73a008d4-1f93-434f-8fbe-3c79b138adb8
date added to LUP
2018-10-20 10:42:23
date last changed
2021-03-31 04:36:10
@article{73a008d4-1f93-434f-8fbe-3c79b138adb8,
  abstract     = {<p>Long double-stranded DNA molecules were separated in microfabricated hexagonal arrays in less than 1 min, several orders of magnitude faster than by using conventional technology. DNA samples were first concentrated at the entrance to the array in a thin band by entropic focusing. They were then separated by pulsed field electrophoresis. T4 (168.9 kbp) and λ (48.5 kbp) DNAs could be resolved into two clearly separated bands in ∼10 s in these experiments. This corresponds to a mass resolution of 6% in 11 min in a 1-cm-long array.</p>},
  author       = {Bakajin, O. and Duke, T. A J and Tegenfeldt, J. and Chou, C. F. and Chan, S. S. and Austin, R. H. and Cox, E. C.},
  issn         = {0003-2700},
  language     = {eng},
  month        = {12},
  number       = {24},
  pages        = {6053--6056},
  publisher    = {The American Chemical Society (ACS)},
  series       = {Analytical Chemistry},
  title        = {Separation of 100-kilobase DNA molecules in 10 seconds},
  url          = {http://dx.doi.org/10.1021/ac015527o},
  doi          = {10.1021/ac015527o},
  volume       = {73},
  year         = {2001},
}