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Making a Hematopoietic Stem Cell

Daniel, Michael G.; Pereira, Carlos Filipe LU ; Lemischka, Ihor R. and Moore, Kateri A. (2016) In Trends in Cell Biology 26(3). p.202-214
Abstract

Previous attempts to either generate or expand hematopoietic stem cells (HSCs) in vitro have involved either ex vivo expansion of pre-existing patient or donor HSCs or de novo generation from pluripotent stem cells (PSCs), comprising both embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). iPSCs alleviated ESC ethical issues but attempts to generate functional mature hematopoietic stem and progenitor cells (HSPCs) have been largely unsuccessful. New efforts focus on directly reprogramming somatic cells into definitive HSCs and HSPCs. To meet clinical needs and to advance drug discovery and stem cell therapy, alternative approaches are necessary. In this review, we synthesize the strategies used and the key findings... (More)

Previous attempts to either generate or expand hematopoietic stem cells (HSCs) in vitro have involved either ex vivo expansion of pre-existing patient or donor HSCs or de novo generation from pluripotent stem cells (PSCs), comprising both embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). iPSCs alleviated ESC ethical issues but attempts to generate functional mature hematopoietic stem and progenitor cells (HSPCs) have been largely unsuccessful. New efforts focus on directly reprogramming somatic cells into definitive HSCs and HSPCs. To meet clinical needs and to advance drug discovery and stem cell therapy, alternative approaches are necessary. In this review, we synthesize the strategies used and the key findings made in recent years by those trying to make an HSC.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Hematopoiesis, Hematopoietic stem cell, Reprogramming, Transcription factors
in
Trends in Cell Biology
volume
26
issue
3
pages
13 pages
publisher
Elsevier Limited
external identifiers
  • scopus:84958672483
ISSN
0962-8924
DOI
10.1016/j.tcb.2015.10.002
language
English
LU publication?
no
id
73dc84cf-1768-46ef-94de-a537e8f87e5d
date added to LUP
2017-10-02 17:24:54
date last changed
2017-11-12 04:35:30
@article{73dc84cf-1768-46ef-94de-a537e8f87e5d,
  abstract     = {<p>Previous attempts to either generate or expand hematopoietic stem cells (HSCs) in vitro have involved either ex vivo expansion of pre-existing patient or donor HSCs or de novo generation from pluripotent stem cells (PSCs), comprising both embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). iPSCs alleviated ESC ethical issues but attempts to generate functional mature hematopoietic stem and progenitor cells (HSPCs) have been largely unsuccessful. New efforts focus on directly reprogramming somatic cells into definitive HSCs and HSPCs. To meet clinical needs and to advance drug discovery and stem cell therapy, alternative approaches are necessary. In this review, we synthesize the strategies used and the key findings made in recent years by those trying to make an HSC.</p>},
  author       = {Daniel, Michael G. and Pereira, Carlos Filipe and Lemischka, Ihor R. and Moore, Kateri A.},
  issn         = {0962-8924},
  keyword      = {Hematopoiesis,Hematopoietic stem cell,Reprogramming,Transcription factors},
  language     = {eng},
  month        = {03},
  number       = {3},
  pages        = {202--214},
  publisher    = {Elsevier Limited},
  series       = {Trends in Cell Biology},
  title        = {Making a Hematopoietic Stem Cell},
  url          = {http://dx.doi.org/10.1016/j.tcb.2015.10.002},
  volume       = {26},
  year         = {2016},
}