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ACAC beta gene (rs2268388) and AGTR1 gene (rs5186) polymorphism and the risk of nephropathy in Asian Indian patients with type 2 diabetes

Shah, Viral N. ; Cheema, Balneek Singh ; Sharma, Rajni ; Khullar, Madhu ; Kohli, Harbir Singh ; Ahluwalia, Tarun LU ; Mohan, Viswanathan and Bhansali, Anil (2013) In Molecular and Cellular Biochemistry 372(1-2). p.191-198
Abstract
Patients with type 2 diabetes (T2DM) are usually obese and concurrent obesity results into activation of the renin-angiotensin-system (RAS) which is a risk factor for diabetic nephropathy (DN). Gene-gene interaction between acetyl-coenzymeA carboxylase beta (ACAC beta) gene, which is involved in fatty acid metabolism and angiotensin II receptors (AGTR1) gene, which mediates RAS proteins actions on renal tissue, polymorphism with DN have not been studied earlier. The present study was designed with the aim to examine the association of an ACAC beta (rs2268388) and AGTR1 (rs5186) gene polymorphism with the risk of DN in Asian Indians. 1,158 patients with T2DM belonging to two independently ascertained North Indian and one South Indian... (More)
Patients with type 2 diabetes (T2DM) are usually obese and concurrent obesity results into activation of the renin-angiotensin-system (RAS) which is a risk factor for diabetic nephropathy (DN). Gene-gene interaction between acetyl-coenzymeA carboxylase beta (ACAC beta) gene, which is involved in fatty acid metabolism and angiotensin II receptors (AGTR1) gene, which mediates RAS proteins actions on renal tissue, polymorphism with DN have not been studied earlier. The present study was designed with the aim to examine the association of an ACAC beta (rs2268388) and AGTR1 (rs5186) gene polymorphism with the risk of DN in Asian Indians. 1,158 patients with T2DM belonging to two independently ascertained North Indian and one South Indian cohorts were genotyped for ACAC beta (rs2268388) and AGTR1 (rs5186) polymorphism using real time PCR-based Taq-man assay and PCR-RFLP assays. In all the three cohorts, a significantly higher frequency of T allele and TT genotypes of ACAC beta and C allele and CC genotypes of AGTR1 were found in patients with DN as compared to patients without nephropathy. Further, T allele of ACAC beta and C allele of AGTR1 were found to be significantly associated with proteinuria, a hallmark of DN. We also found significant epistatic interactions between these two genes. TT genotypes of ACAC beta gene and CC genotype of AGTR1 gene confers the risk of DN and both genes had significant epistatic interaction in Asian Indian patients with T2DM. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Acetyl-CoA carboxylase beta, Angiotensin II type 1 receptor, Gene, polymorphism, Diabetic nephropathy, Asian Indian
in
Molecular and Cellular Biochemistry
volume
372
issue
1-2
pages
191 - 198
publisher
Springer
external identifiers
  • wos:000312130500021
  • scopus:84870416376
  • pmid:23081748
ISSN
0300-8177
DOI
10.1007/s11010-012-1460-2
language
English
LU publication?
yes
id
73e4f284-391c-40aa-b111-8557d3efba1f (old id 3400979)
date added to LUP
2016-04-01 14:20:32
date last changed
2024-03-27 15:41:54
@article{73e4f284-391c-40aa-b111-8557d3efba1f,
  abstract     = {{Patients with type 2 diabetes (T2DM) are usually obese and concurrent obesity results into activation of the renin-angiotensin-system (RAS) which is a risk factor for diabetic nephropathy (DN). Gene-gene interaction between acetyl-coenzymeA carboxylase beta (ACAC beta) gene, which is involved in fatty acid metabolism and angiotensin II receptors (AGTR1) gene, which mediates RAS proteins actions on renal tissue, polymorphism with DN have not been studied earlier. The present study was designed with the aim to examine the association of an ACAC beta (rs2268388) and AGTR1 (rs5186) gene polymorphism with the risk of DN in Asian Indians. 1,158 patients with T2DM belonging to two independently ascertained North Indian and one South Indian cohorts were genotyped for ACAC beta (rs2268388) and AGTR1 (rs5186) polymorphism using real time PCR-based Taq-man assay and PCR-RFLP assays. In all the three cohorts, a significantly higher frequency of T allele and TT genotypes of ACAC beta and C allele and CC genotypes of AGTR1 were found in patients with DN as compared to patients without nephropathy. Further, T allele of ACAC beta and C allele of AGTR1 were found to be significantly associated with proteinuria, a hallmark of DN. We also found significant epistatic interactions between these two genes. TT genotypes of ACAC beta gene and CC genotype of AGTR1 gene confers the risk of DN and both genes had significant epistatic interaction in Asian Indian patients with T2DM.}},
  author       = {{Shah, Viral N. and Cheema, Balneek Singh and Sharma, Rajni and Khullar, Madhu and Kohli, Harbir Singh and Ahluwalia, Tarun and Mohan, Viswanathan and Bhansali, Anil}},
  issn         = {{0300-8177}},
  keywords     = {{Acetyl-CoA carboxylase beta; Angiotensin II type 1 receptor; Gene; polymorphism; Diabetic nephropathy; Asian Indian}},
  language     = {{eng}},
  number       = {{1-2}},
  pages        = {{191--198}},
  publisher    = {{Springer}},
  series       = {{Molecular and Cellular Biochemistry}},
  title        = {{ACAC beta gene (rs2268388) and AGTR1 gene (rs5186) polymorphism and the risk of nephropathy in Asian Indian patients with type 2 diabetes}},
  url          = {{http://dx.doi.org/10.1007/s11010-012-1460-2}},
  doi          = {{10.1007/s11010-012-1460-2}},
  volume       = {{372}},
  year         = {{2013}},
}