Advanced

Horizontal gene transfer promoted the evolution of the ability to propagate under anaerobic conditions

Gojkovic, Zoran; Knecht, W.; Zameitat, E.; Warneboldt, J.; Coutelis, J.-B.; Pynyaha, Y.; Neuveglise, C.; Moeller, K.; Löffler, M. and Piskur, Jure LU (2004) In Molecular Genetics and Genomics2001-01-01+01:00 271(4). p.387-393
Abstract
The ability to propagate under anaerobic conditions

is an essential and unique trait of brewer’s or

baker’s yeast (Saccharomyces cervisiae). To understand

the evolution of facultative anaerobiosis we studied the

dependence of de novo pyrimidine biosynthesis, more

precisely the fourth enzymic activity catalysed by dihydroorotate

dehydrogenase (DHODase), on the enzymes

of the respiratory chain in several yeast species. While

the majority of yeasts possess a mitochondrial DHODase,

Saccharomyces cerevisiae has a cytoplasmatic enzyme,

whose activity is independent of the presence of

oxygen. From the phylogenetic point of view, this ... (More)
The ability to propagate under anaerobic conditions

is an essential and unique trait of brewer’s or

baker’s yeast (Saccharomyces cervisiae). To understand

the evolution of facultative anaerobiosis we studied the

dependence of de novo pyrimidine biosynthesis, more

precisely the fourth enzymic activity catalysed by dihydroorotate

dehydrogenase (DHODase), on the enzymes

of the respiratory chain in several yeast species. While

the majority of yeasts possess a mitochondrial DHODase,

Saccharomyces cerevisiae has a cytoplasmatic enzyme,

whose activity is independent of the presence of

oxygen. From the phylogenetic point of view, this enzyme

is closely related to a bacterial DHODase from

Lactococcus lactis. Here we show that S. kluyveri, which

separated from the S. cerevisiae lineage more than 100

million years ago, represents an evolutionary intermediate,

having both cytoplasmic and mitochondrial

DHODases. We show that these two S. kluyveri enzymes,

and their coding genes, differ in their dependence

on the presence of oxygen. Only the cytoplasmic

DHODase promotes growth in the absence of oxygen.

Apparently a Saccharomyces yeast progenitor which had

a eukaryotic-like mitochondrial DHODase acquired a

bacterial gene for DHODase, which subsequently

allowed cell growth gradually to become independent of

oxygen. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
nucleic acid precursors, yeast, horizontal transfer, evolution, Anaerobiosis, Pyrimidines
in
Molecular Genetics and Genomics2001-01-01+01:00
volume
271
issue
4
pages
387 - 393
publisher
Springer
external identifiers
  • scopus:3042677643
ISSN
1617-4615
DOI
10.1007/s00438-004-0995-7
language
English
LU publication?
yes
id
96602576-4933-4636-bbf1-f5eb594ebd4f (old id 740192)
date added to LUP
2008-01-09 13:10:32
date last changed
2017-12-03 03:20:29
@article{96602576-4933-4636-bbf1-f5eb594ebd4f,
  abstract     = {The ability to propagate under anaerobic conditions<br/><br>
is an essential and unique trait of brewer’s or<br/><br>
baker’s yeast (Saccharomyces cervisiae). To understand<br/><br>
the evolution of facultative anaerobiosis we studied the<br/><br>
dependence of de novo pyrimidine biosynthesis, more<br/><br>
precisely the fourth enzymic activity catalysed by dihydroorotate<br/><br>
dehydrogenase (DHODase), on the enzymes<br/><br>
of the respiratory chain in several yeast species. While<br/><br>
the majority of yeasts possess a mitochondrial DHODase,<br/><br>
Saccharomyces cerevisiae has a cytoplasmatic enzyme,<br/><br>
whose activity is independent of the presence of<br/><br>
oxygen. From the phylogenetic point of view, this enzyme<br/><br>
is closely related to a bacterial DHODase from<br/><br>
Lactococcus lactis. Here we show that S. kluyveri, which<br/><br>
separated from the S. cerevisiae lineage more than 100<br/><br>
million years ago, represents an evolutionary intermediate,<br/><br>
having both cytoplasmic and mitochondrial<br/><br>
DHODases. We show that these two S. kluyveri enzymes,<br/><br>
and their coding genes, differ in their dependence<br/><br>
on the presence of oxygen. Only the cytoplasmic<br/><br>
DHODase promotes growth in the absence of oxygen.<br/><br>
Apparently a Saccharomyces yeast progenitor which had<br/><br>
a eukaryotic-like mitochondrial DHODase acquired a<br/><br>
bacterial gene for DHODase, which subsequently<br/><br>
allowed cell growth gradually to become independent of<br/><br>
oxygen.},
  author       = {Gojkovic, Zoran and Knecht, W. and Zameitat, E. and Warneboldt, J. and Coutelis, J.-B. and Pynyaha, Y. and Neuveglise, C. and Moeller, K. and Löffler, M. and Piskur, Jure},
  issn         = {1617-4615},
  keyword      = {nucleic acid precursors,yeast,horizontal transfer,evolution,Anaerobiosis,Pyrimidines},
  language     = {eng},
  number       = {4},
  pages        = {387--393},
  publisher    = {Springer},
  series       = {Molecular Genetics and Genomics2001-01-01+01:00},
  title        = {Horizontal gene transfer promoted the evolution of the ability to propagate under anaerobic conditions},
  url          = {http://dx.doi.org/10.1007/s00438-004-0995-7},
  volume       = {271},
  year         = {2004},
}