Neutrophil secretion induced by an intracellular Ca2+ rise and followed by whole-cell patch-clamp recordings occurs without any selective mobilization of different granule populations
(2006) In Journal of Biomedicine and Biotechnology p.97803-97803- Abstract
- We have investigated calcium-induced secretion in human neutrophils, using a whole-cell patch-clamp technique. Mobilization of subcellular granules to the cell membrane was followed as the change in membrane capacitance (Delta C-m). Both the magnitude and the kinetics of the response differed between low and high concentrations of Ca2+. A sustained secretion following a short lag phase was induced by high concentrations of Ca2+ (100 mu M and higher). A stable plateau was reached after 5-7 minutes at Delta C-m values corresponding to values expected after all specific as well as azurophil granules have been mobilized. Capacitance values of the same magnitude could be obtained also at lower Ca2+ concentrations, but typically no stable... (More)
- We have investigated calcium-induced secretion in human neutrophils, using a whole-cell patch-clamp technique. Mobilization of subcellular granules to the cell membrane was followed as the change in membrane capacitance (Delta C-m). Both the magnitude and the kinetics of the response differed between low and high concentrations of Ca2+. A sustained secretion following a short lag phase was induced by high concentrations of Ca2+ (100 mu M and higher). A stable plateau was reached after 5-7 minutes at Delta C-m values corresponding to values expected after all specific as well as azurophil granules have been mobilized. Capacitance values of the same magnitude could be obtained also at lower Ca2+ concentrations, but typically no stable plateau was reached within the measuring time. In contrast to previous studies, we were unable to detect any pattern of secretion corresponding to a distinct submaximal response or selective mobilization of granule subsets specified by their Ca2+-sensitivity. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/7410299
- author
- Granfeldt, Daniel ; Harbecke, Olof LU ; Bjorstad, Ase ; Karlsson, Anna and Dahlgren, Claes
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Biomedicine and Biotechnology
- pages
- 97803 - 97803
- publisher
- Hindawi Limited
- external identifiers
-
- wos:000240341600001
- scopus:33746790606
- ISSN
- 1110-7251
- DOI
- 10.1155/JBB/2006/97803
- language
- English
- LU publication?
- yes
- id
- febeb554-d157-410a-acb2-3af97b8d4e26 (old id 7410299)
- date added to LUP
- 2016-04-01 11:54:58
- date last changed
- 2022-01-26 20:10:29
@article{febeb554-d157-410a-acb2-3af97b8d4e26,
abstract = {{We have investigated calcium-induced secretion in human neutrophils, using a whole-cell patch-clamp technique. Mobilization of subcellular granules to the cell membrane was followed as the change in membrane capacitance (Delta C-m). Both the magnitude and the kinetics of the response differed between low and high concentrations of Ca2+. A sustained secretion following a short lag phase was induced by high concentrations of Ca2+ (100 mu M and higher). A stable plateau was reached after 5-7 minutes at Delta C-m values corresponding to values expected after all specific as well as azurophil granules have been mobilized. Capacitance values of the same magnitude could be obtained also at lower Ca2+ concentrations, but typically no stable plateau was reached within the measuring time. In contrast to previous studies, we were unable to detect any pattern of secretion corresponding to a distinct submaximal response or selective mobilization of granule subsets specified by their Ca2+-sensitivity.}},
author = {{Granfeldt, Daniel and Harbecke, Olof and Bjorstad, Ase and Karlsson, Anna and Dahlgren, Claes}},
issn = {{1110-7251}},
language = {{eng}},
pages = {{97803--97803}},
publisher = {{Hindawi Limited}},
series = {{Journal of Biomedicine and Biotechnology}},
title = {{Neutrophil secretion induced by an intracellular Ca2+ rise and followed by whole-cell patch-clamp recordings occurs without any selective mobilization of different granule populations}},
url = {{http://dx.doi.org/10.1155/JBB/2006/97803}},
doi = {{10.1155/JBB/2006/97803}},
year = {{2006}},
}