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Neutrophil secretion induced by an intracellular Ca2+ rise and followed by whole-cell patch-clamp recordings occurs without any selective mobilization of different granule populations

Granfeldt, Daniel ; Harbecke, Olof LU ; Bjorstad, Ase ; Karlsson, Anna and Dahlgren, Claes (2006) In Journal of Biomedicine and Biotechnology p.97803-97803
Abstract
We have investigated calcium-induced secretion in human neutrophils, using a whole-cell patch-clamp technique. Mobilization of subcellular granules to the cell membrane was followed as the change in membrane capacitance (Delta C-m). Both the magnitude and the kinetics of the response differed between low and high concentrations of Ca2+. A sustained secretion following a short lag phase was induced by high concentrations of Ca2+ (100 mu M and higher). A stable plateau was reached after 5-7 minutes at Delta C-m values corresponding to values expected after all specific as well as azurophil granules have been mobilized. Capacitance values of the same magnitude could be obtained also at lower Ca2+ concentrations, but typically no stable... (More)
We have investigated calcium-induced secretion in human neutrophils, using a whole-cell patch-clamp technique. Mobilization of subcellular granules to the cell membrane was followed as the change in membrane capacitance (Delta C-m). Both the magnitude and the kinetics of the response differed between low and high concentrations of Ca2+. A sustained secretion following a short lag phase was induced by high concentrations of Ca2+ (100 mu M and higher). A stable plateau was reached after 5-7 minutes at Delta C-m values corresponding to values expected after all specific as well as azurophil granules have been mobilized. Capacitance values of the same magnitude could be obtained also at lower Ca2+ concentrations, but typically no stable plateau was reached within the measuring time. In contrast to previous studies, we were unable to detect any pattern of secretion corresponding to a distinct submaximal response or selective mobilization of granule subsets specified by their Ca2+-sensitivity. (Less)
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publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Biomedicine and Biotechnology
pages
97803 - 97803
publisher
Hindawi Limited
external identifiers
  • wos:000240341600001
  • scopus:33746790606
ISSN
1110-7251
DOI
10.1155/JBB/2006/97803
language
English
LU publication?
yes
id
febeb554-d157-410a-acb2-3af97b8d4e26 (old id 7410299)
date added to LUP
2016-04-01 11:54:58
date last changed
2021-02-17 04:52:44
@article{febeb554-d157-410a-acb2-3af97b8d4e26,
  abstract     = {We have investigated calcium-induced secretion in human neutrophils, using a whole-cell patch-clamp technique. Mobilization of subcellular granules to the cell membrane was followed as the change in membrane capacitance (Delta C-m). Both the magnitude and the kinetics of the response differed between low and high concentrations of Ca2+. A sustained secretion following a short lag phase was induced by high concentrations of Ca2+ (100 mu M and higher). A stable plateau was reached after 5-7 minutes at Delta C-m values corresponding to values expected after all specific as well as azurophil granules have been mobilized. Capacitance values of the same magnitude could be obtained also at lower Ca2+ concentrations, but typically no stable plateau was reached within the measuring time. In contrast to previous studies, we were unable to detect any pattern of secretion corresponding to a distinct submaximal response or selective mobilization of granule subsets specified by their Ca2+-sensitivity.},
  author       = {Granfeldt, Daniel and Harbecke, Olof and Bjorstad, Ase and Karlsson, Anna and Dahlgren, Claes},
  issn         = {1110-7251},
  language     = {eng},
  pages        = {97803--97803},
  publisher    = {Hindawi Limited},
  series       = {Journal of Biomedicine and Biotechnology},
  title        = {Neutrophil secretion induced by an intracellular Ca2+ rise and followed by whole-cell patch-clamp recordings occurs without any selective mobilization of different granule populations},
  url          = {http://dx.doi.org/10.1155/JBB/2006/97803},
  doi          = {10.1155/JBB/2006/97803},
  year         = {2006},
}