Design and Construction of the Lawnmower, An Artificial Burnt-Bridges Motor
Kovacic, Suzana ; Samii, Laleh ; Curmi, Paul M. G. ; Linke, Heiner LU
; Zuckermann, Martin J.
and Forde, Nancy R.
(2015)
In IEEE Transactions on Nanobioscience
14(3).
p.305-312
- Abstract
- Molecular motors of the cell are protein-based, nanoscale machines, which use a variety of strategies to transduce chemical energy into mechanical work in the presence of a large thermal background. The design and construction of artificial molecular motors is one approach to better understand their basic physical principles. Here, we propose the concept of a protein-based, burnt-bridges ratchet, inspired by biological examples. Our concept, the lawnmower, utilizes protease blades to cleave peptide substrates, and uses the asymmetric substrate-product interface arising from productive cleavage to bias subsequent diffusion on the track (lawn). Following experimental screening to select a protease to act as the motor's blades, we chemically... (More)
- Molecular motors of the cell are protein-based, nanoscale machines, which use a variety of strategies to transduce chemical energy into mechanical work in the presence of a large thermal background. The design and construction of artificial molecular motors is one approach to better understand their basic physical principles. Here, we propose the concept of a protein-based, burnt-bridges ratchet, inspired by biological examples. Our concept, the lawnmower, utilizes protease blades to cleave peptide substrates, and uses the asymmetric substrate-product interface arising from productive cleavage to bias subsequent diffusion on the track (lawn). Following experimental screening to select a protease to act as the motor's blades, we chemically couple trypsin to quantum dots and demonstrate activity of the resulting lawnmower construct in solution. Accompanying Brownian dynamics simulations illustrate the importance for processivity of correct protease density on the quantum dot and spacing of substrates on the track. These results lay the groundwork for future tests of the protein-based lawnmower's motor performance characteristics. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/7411005
- author
- Kovacic, Suzana
; Samii, Laleh
; Curmi, Paul M. G.
; Linke, Heiner
LU
; Zuckermann, Martin J.
and Forde, Nancy R.
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Burnt-bridges, molecular motor, protein, quantum dot, trypsin
- in
- IEEE Transactions on Nanobioscience
- volume
- 14
- issue
- 3
- pages
- 305 - 312
- publisher
- IEEE - Institute of Electrical and Electronics Engineers Inc.
- external identifiers
-
- wos:000355321100007
- scopus:84930625344
- pmid:25751869
- ISSN
- 1558-2639
- DOI
- 10.1109/TNB.2015.2393872
- language
- English
- LU publication?
- yes
- id
- 8bfc1379-f49d-4807-b651-ee119fcea2ff (old id 7411005)
- date added to LUP
- 2016-04-01 10:31:47
- date last changed
- 2023-09-28 07:52:31
@article{8bfc1379-f49d-4807-b651-ee119fcea2ff,
abstract = {{Molecular motors of the cell are protein-based, nanoscale machines, which use a variety of strategies to transduce chemical energy into mechanical work in the presence of a large thermal background. The design and construction of artificial molecular motors is one approach to better understand their basic physical principles. Here, we propose the concept of a protein-based, burnt-bridges ratchet, inspired by biological examples. Our concept, the lawnmower, utilizes protease blades to cleave peptide substrates, and uses the asymmetric substrate-product interface arising from productive cleavage to bias subsequent diffusion on the track (lawn). Following experimental screening to select a protease to act as the motor's blades, we chemically couple trypsin to quantum dots and demonstrate activity of the resulting lawnmower construct in solution. Accompanying Brownian dynamics simulations illustrate the importance for processivity of correct protease density on the quantum dot and spacing of substrates on the track. These results lay the groundwork for future tests of the protein-based lawnmower's motor performance characteristics.}},
author = {{Kovacic, Suzana and Samii, Laleh and Curmi, Paul M. G. and Linke, Heiner and Zuckermann, Martin J. and Forde, Nancy R.}},
issn = {{1558-2639}},
keywords = {{Burnt-bridges; molecular motor; protein; quantum dot; trypsin}},
language = {{eng}},
number = {{3}},
pages = {{305--312}},
publisher = {{IEEE - Institute of Electrical and Electronics Engineers Inc.}},
series = {{IEEE Transactions on Nanobioscience}},
title = {{Design and Construction of the Lawnmower, An Artificial Burnt-Bridges Motor}},
url = {{http://dx.doi.org/10.1109/TNB.2015.2393872}},
doi = {{10.1109/TNB.2015.2393872}},
volume = {{14}},
year = {{2015}},
}