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Methods for autofluorescence analysis of uterine cavity washings

Gegzna, V.; Sladkevicius, P.; Valentin, Lil LU and Vaitkuviene, A. (2015) In Lithuanian Journal of Physics 55(1). p.63-70
Abstract
The diagnostics of endometrial pathology can be done by obtaining information at the molecular level, e. g. using the autofluorescence-based technique. Thus, for the first time an experimental study was performed on waste material of uterine cavity washing specimens to evaluate suitability of the technique for diagnostics. The specimens were obtained from 32 patients who had a conventional uterine hydrosonography procedure. A portable Nd:YAG 355 nm microlaser was used to excite autofluorescence at the point of care. Various algorithms of multivariate curve resolution and artificial neural networks were utilized for spectra analysis. The spectra were classified according to histological and ultrasound diagnosis. Receiver operating... (More)
The diagnostics of endometrial pathology can be done by obtaining information at the molecular level, e. g. using the autofluorescence-based technique. Thus, for the first time an experimental study was performed on waste material of uterine cavity washing specimens to evaluate suitability of the technique for diagnostics. The specimens were obtained from 32 patients who had a conventional uterine hydrosonography procedure. A portable Nd:YAG 355 nm microlaser was used to excite autofluorescence at the point of care. Various algorithms of multivariate curve resolution and artificial neural networks were utilized for spectra analysis. The spectra were classified according to histological and ultrasound diagnosis. Receiver operating characteristic (ROC) curve analysis was used to make statistical decisions. The results showed that it was possible to distinguish all compared groups: pathologic vs non-pathologic endometrium (sensitivity 97.3 +/- 5.2%, specificity 91.7 +/- 7%, AUC (area under the ROC curve) 0.96 +/- 0.04), malignant endometrium vs endometrial polyps (sensitivity 100 +/- 0%, specificity 92.0 +/- 10.6%, AUG = 0.98 +/- 0.07), and secretory menstrual cycle phase vs proliferative phase (sensitivity 87.5 +/- 13.2%, specificity 94.4 +/- 7.4%, AUG = 0.88 +/- 0.10). To conclude, uterine cavity washing specimens could be used for endometrial pathology recognition using the autofluorescence-based technique in clinical setting. It will possibly speed up the treatment decision making for endometrial pathology. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
endometrium, tissue fluorescence, photodiagnostics, multivariate, analysis
in
Lithuanian Journal of Physics
volume
55
issue
1
pages
63 - 70
publisher
Lithuanian Physical Society
external identifiers
  • wos:000353856200008
  • scopus:84928675876
ISSN
1648-8504
DOI
10.3952/physics.v55i1.3059
language
English
LU publication?
yes
id
d93d1c55-f044-4aec-96e3-fb33930ae69b (old id 7439484)
date added to LUP
2015-07-03 07:10:27
date last changed
2017-01-01 06:00:35
@article{d93d1c55-f044-4aec-96e3-fb33930ae69b,
  abstract     = {The diagnostics of endometrial pathology can be done by obtaining information at the molecular level, e. g. using the autofluorescence-based technique. Thus, for the first time an experimental study was performed on waste material of uterine cavity washing specimens to evaluate suitability of the technique for diagnostics. The specimens were obtained from 32 patients who had a conventional uterine hydrosonography procedure. A portable Nd:YAG 355 nm microlaser was used to excite autofluorescence at the point of care. Various algorithms of multivariate curve resolution and artificial neural networks were utilized for spectra analysis. The spectra were classified according to histological and ultrasound diagnosis. Receiver operating characteristic (ROC) curve analysis was used to make statistical decisions. The results showed that it was possible to distinguish all compared groups: pathologic vs non-pathologic endometrium (sensitivity 97.3 +/- 5.2%, specificity 91.7 +/- 7%, AUC (area under the ROC curve) 0.96 +/- 0.04), malignant endometrium vs endometrial polyps (sensitivity 100 +/- 0%, specificity 92.0 +/- 10.6%, AUG = 0.98 +/- 0.07), and secretory menstrual cycle phase vs proliferative phase (sensitivity 87.5 +/- 13.2%, specificity 94.4 +/- 7.4%, AUG = 0.88 +/- 0.10). To conclude, uterine cavity washing specimens could be used for endometrial pathology recognition using the autofluorescence-based technique in clinical setting. It will possibly speed up the treatment decision making for endometrial pathology.},
  author       = {Gegzna, V. and Sladkevicius, P. and Valentin, Lil and Vaitkuviene, A.},
  issn         = {1648-8504},
  keyword      = {endometrium,tissue fluorescence,photodiagnostics,multivariate,analysis},
  language     = {eng},
  number       = {1},
  pages        = {63--70},
  publisher    = {Lithuanian Physical Society},
  series       = {Lithuanian Journal of Physics},
  title        = {Methods for autofluorescence analysis of uterine cavity washings},
  url          = {http://dx.doi.org/10.3952/physics.v55i1.3059},
  volume       = {55},
  year         = {2015},
}