Advanced

Macrophage and dendritic cell subsets in IBD: ALDH(+) cells are reduced in colon tissue of patients with ulcerative colitis regardless of inflammation.

Magnusson, M K; Brynjólfsson, S F; Dige, A; Uronen-Hansson, Heli LU ; Börjesson, L G; Bengtsson, J L; Gudjonsson, Sigurdur LU ; Öhman, L; Agnholt, J and Sjövall, H, et al. (2016) In Mucosal Immunology 9(1). p.171-182
Abstract
Disruption of the homeostatic balance of intestinal dendritic cells (DCs) and macrophages (MQs) may contribute to inflammatory bowel disease. We characterized DC and MQ populations, including their ability to produce retinoic acid, in clinical material encompassing Crohn's ileitis, Crohn's colitis and ulcerative colitis (UC) as well as mesenteric lymph nodes (MLNs) draining these sites. Increased CD14(+)DR(int) MQs characterized inflamed intestinal mucosa while total CD141(+) or CD1c(+) DCs numbers were unchanged. However, CD103(+) DCs, including CD141(+)CD103(+) and CD1c(+)CD103(+) DCs, were reduced in inflamed intestine. In MLNs, two CD14(-) DC populations were identified: CD11c(int)HLADR(hi) and CD11c(hi)HLADR(int) cells. A marked... (More)
Disruption of the homeostatic balance of intestinal dendritic cells (DCs) and macrophages (MQs) may contribute to inflammatory bowel disease. We characterized DC and MQ populations, including their ability to produce retinoic acid, in clinical material encompassing Crohn's ileitis, Crohn's colitis and ulcerative colitis (UC) as well as mesenteric lymph nodes (MLNs) draining these sites. Increased CD14(+)DR(int) MQs characterized inflamed intestinal mucosa while total CD141(+) or CD1c(+) DCs numbers were unchanged. However, CD103(+) DCs, including CD141(+)CD103(+) and CD1c(+)CD103(+) DCs, were reduced in inflamed intestine. In MLNs, two CD14(-) DC populations were identified: CD11c(int)HLADR(hi) and CD11c(hi)HLADR(int) cells. A marked increase of CD11c(hi)HLADR(int) DC, particularly DR(int)CD1c(+) DCs, characterized MLNs draining inflamed intestine. The fraction of DC and MQ populations expressing aldehyde dehydrogenase (ALDH) activity, reflecting retinoic acid synthesis, in UC colon, both in active disease and remission, were reduced compared to controls and inflamed Crohn's colon. In contrast, no difference in the frequency of ALDH(+) cells among blood precursors was detected between UC patients and non-inflamed controls. This suggests that ALDH activity in myeloid cells in the colon of UC patients, regardless of whether the disease is active or in remission, is influenced by the intestinal environment.Mucosal Immunology advance online publication, 17 June 2015; doi:10.1038/mi.2015.48. (Less)
Please use this url to cite or link to this publication:
author
, et al. (More)
(Less)
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Mucosal Immunology
volume
9
issue
1
pages
171 - 182
publisher
Nature Publishing Group
external identifiers
  • pmid:26080709
  • wos:000367653800014
  • scopus:84953227102
ISSN
1933-0219
DOI
10.1038/mi.2015.48
language
English
LU publication?
yes
id
cadf5bc4-8402-45d8-b674-2ac8eb9ca462 (old id 7485518)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26080709?dopt=Abstract
date added to LUP
2015-07-08 18:06:28
date last changed
2017-11-19 03:20:21
@article{cadf5bc4-8402-45d8-b674-2ac8eb9ca462,
  abstract     = {Disruption of the homeostatic balance of intestinal dendritic cells (DCs) and macrophages (MQs) may contribute to inflammatory bowel disease. We characterized DC and MQ populations, including their ability to produce retinoic acid, in clinical material encompassing Crohn's ileitis, Crohn's colitis and ulcerative colitis (UC) as well as mesenteric lymph nodes (MLNs) draining these sites. Increased CD14(+)DR(int) MQs characterized inflamed intestinal mucosa while total CD141(+) or CD1c(+) DCs numbers were unchanged. However, CD103(+) DCs, including CD141(+)CD103(+) and CD1c(+)CD103(+) DCs, were reduced in inflamed intestine. In MLNs, two CD14(-) DC populations were identified: CD11c(int)HLADR(hi) and CD11c(hi)HLADR(int) cells. A marked increase of CD11c(hi)HLADR(int) DC, particularly DR(int)CD1c(+) DCs, characterized MLNs draining inflamed intestine. The fraction of DC and MQ populations expressing aldehyde dehydrogenase (ALDH) activity, reflecting retinoic acid synthesis, in UC colon, both in active disease and remission, were reduced compared to controls and inflamed Crohn's colon. In contrast, no difference in the frequency of ALDH(+) cells among blood precursors was detected between UC patients and non-inflamed controls. This suggests that ALDH activity in myeloid cells in the colon of UC patients, regardless of whether the disease is active or in remission, is influenced by the intestinal environment.Mucosal Immunology advance online publication, 17 June 2015; doi:10.1038/mi.2015.48.},
  author       = {Magnusson, M K and Brynjólfsson, S F and Dige, A and Uronen-Hansson, Heli and Börjesson, L G and Bengtsson, J L and Gudjonsson, Sigurdur and Öhman, L and Agnholt, J and Sjövall, H and Agace, William and Wick, M J},
  issn         = {1933-0219},
  language     = {eng},
  number       = {1},
  pages        = {171--182},
  publisher    = {Nature Publishing Group},
  series       = {Mucosal Immunology},
  title        = {Macrophage and dendritic cell subsets in IBD: ALDH(+) cells are reduced in colon tissue of patients with ulcerative colitis regardless of inflammation.},
  url          = {http://dx.doi.org/10.1038/mi.2015.48},
  volume       = {9},
  year         = {2016},
}