An Improvement on MS-based Epigenetic Analysis of Large Histone-derived Peptides by Using the IVU Interface.
(2015) In Analytical Biochemistry 486(Online 05 June 2015). p.14-16- Abstract
- Highly protonated histone-derived peptides impede a sufficient mass spectrometry (MS) -based epigenetic analysis since their relatively low m/z due to a high degree of proton addition to peptides would difficult to analyze the resulting complex MS/MS spectra. In order to reduce the degree of protonations, we have developed a new interface, the IVU, in which peptides are ionized under a vaporized organic solvent, and it has demonstrated that the doubly-charged histone-tail H2B peptide, PEPAKSAPAPKKGSKKAVTKAQKK (m/z 1238.243 , +2), has been detected by using the IVU interface and sequenced, which was not detectable before.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/7487715
- author
- Fukuda, Tetsuya ; Hike, Hiroshi ; Usui, Fumihiko ; Bando, Yasuhiko ; Nishimura, Toshide LU ; Kodama, Tatsuhiko and Kawamura, Takeshi
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical Biochemistry
- volume
- 486
- issue
- Online 05 June 2015
- pages
- 14 - 16
- publisher
- Elsevier
- external identifiers
-
- pmid:26050628
- wos:000359881000003
- scopus:84937551726
- pmid:26050628
- ISSN
- 1096-0309
- DOI
- 10.1016/j.ab.2015.06.002
- language
- English
- LU publication?
- yes
- id
- 635dfa16-c94f-4228-9e80-39735122aea3 (old id 7487715)
- date added to LUP
- 2016-04-01 10:47:41
- date last changed
- 2022-01-26 02:32:33
@article{635dfa16-c94f-4228-9e80-39735122aea3, abstract = {{Highly protonated histone-derived peptides impede a sufficient mass spectrometry (MS) -based epigenetic analysis since their relatively low m/z due to a high degree of proton addition to peptides would difficult to analyze the resulting complex MS/MS spectra. In order to reduce the degree of protonations, we have developed a new interface, the IVU, in which peptides are ionized under a vaporized organic solvent, and it has demonstrated that the doubly-charged histone-tail H2B peptide, PEPAKSAPAPKKGSKKAVTKAQKK (m/z 1238.243 , +2), has been detected by using the IVU interface and sequenced, which was not detectable before.}}, author = {{Fukuda, Tetsuya and Hike, Hiroshi and Usui, Fumihiko and Bando, Yasuhiko and Nishimura, Toshide and Kodama, Tatsuhiko and Kawamura, Takeshi}}, issn = {{1096-0309}}, language = {{eng}}, number = {{Online 05 June 2015}}, pages = {{14--16}}, publisher = {{Elsevier}}, series = {{Analytical Biochemistry}}, title = {{An Improvement on MS-based Epigenetic Analysis of Large Histone-derived Peptides by Using the IVU Interface.}}, url = {{http://dx.doi.org/10.1016/j.ab.2015.06.002}}, doi = {{10.1016/j.ab.2015.06.002}}, volume = {{486}}, year = {{2015}}, }