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Carbon Aerogel as Electrode Material for Improved Direct Electron Transfer in Biosensors Incorporating Cellobiose Dehydrogenase

Fort, Carmen Ioana; Ortiz, Roberto LU ; Cotet, Liviu Cosmin; Danciu, Virginia; Popescu, Ionel Catalin and Gorton, Lo LU (2016) In Electroanalysis 28(10). p.2311-2319
Abstract

High mesoporous carbon aerogels (CA) were prepared by a sol-gel method, followed by supercritical drying with liquid CO2 and pyrolysis under inert atmosphere. The morphological and structural characteristics (surface area, pore size distribution and pore volume) of CA were examined by using N2 adsorption/desorption isotherms, TEM, SEM and AFM measurements. CA, dispersed in a chitosan (Chi) solution, was immobilized on the surface of a glassy carbon (GC) electrode aiming to prepare an amperometric transducer able to improve the direct electron transfer (DET) between Phanerochaete chrysosporium cellobiose dehydrogenase (PcCDH) and the electrode. The resulting GC/CA-Chi/PcCDH biosensor was optimized with regards to... (More)

High mesoporous carbon aerogels (CA) were prepared by a sol-gel method, followed by supercritical drying with liquid CO2 and pyrolysis under inert atmosphere. The morphological and structural characteristics (surface area, pore size distribution and pore volume) of CA were examined by using N2 adsorption/desorption isotherms, TEM, SEM and AFM measurements. CA, dispersed in a chitosan (Chi) solution, was immobilized on the surface of a glassy carbon (GC) electrode aiming to prepare an amperometric transducer able to improve the direct electron transfer (DET) between Phanerochaete chrysosporium cellobiose dehydrogenase (PcCDH) and the electrode. The resulting GC/CA-Chi/PcCDH biosensor was optimized with regards to the loading of CA and PcCDH, as well as pH of the buffer solution. The electroanalytical parameters of the optimized biosensor (maximum current responses (Imax), apparent Michaelis-Menten constants (KM)) were estimated from amperometric calibration curves for lactose. The remarkably high sensitivity towards lactose (133.7±0.2 µA cm−2 mM−1) at the GC/CA-Chi/PcCDH electrode recommends the GC/CA-Chi electrode as an efficient transducer for PcCDH based biosensors, exploiting DET.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
amperometric biosensors, carbon aerogel, cellobiose dehydrogenase, direct electron transfer, lactose
in
Electroanalysis
volume
28
issue
10
pages
9 pages
publisher
John Wiley & Sons
external identifiers
  • scopus:84977125988
  • wos:000387886500008
ISSN
1040-0397
DOI
10.1002/elan.201600219
language
English
LU publication?
yes
id
74963539-590b-4fc3-8ce9-7cfd82c5de10
date added to LUP
2016-10-25 13:21:12
date last changed
2017-09-24 05:02:11
@article{74963539-590b-4fc3-8ce9-7cfd82c5de10,
  abstract     = {<p>High mesoporous carbon aerogels (CA) were prepared by a sol-gel method, followed by supercritical drying with liquid CO<sub>2</sub> and pyrolysis under inert atmosphere. The morphological and structural characteristics (surface area, pore size distribution and pore volume) of CA were examined by using N<sub>2</sub> adsorption/desorption isotherms, TEM, SEM and AFM measurements. CA, dispersed in a chitosan (Chi) solution, was immobilized on the surface of a glassy carbon (GC) electrode aiming to prepare an amperometric transducer able to improve the direct electron transfer (DET) between Phanerochaete chrysosporium cellobiose dehydrogenase (PcCDH) and the electrode. The resulting GC/CA-Chi/PcCDH biosensor was optimized with regards to the loading of CA and PcCDH, as well as pH of the buffer solution. The electroanalytical parameters of the optimized biosensor (maximum current responses (I<sub>max</sub>), apparent Michaelis-Menten constants (K<sub>M</sub>)) were estimated from amperometric calibration curves for lactose. The remarkably high sensitivity towards lactose (133.7±0.2 µA cm<sup>−2</sup> mM<sup>−1</sup>) at the GC/CA-Chi/PcCDH electrode recommends the GC/CA-Chi electrode as an efficient transducer for PcCDH based biosensors, exploiting DET.</p>},
  author       = {Fort, Carmen Ioana and Ortiz, Roberto and Cotet, Liviu Cosmin and Danciu, Virginia and Popescu, Ionel Catalin and Gorton, Lo},
  issn         = {1040-0397},
  keyword      = {amperometric biosensors,carbon aerogel,cellobiose dehydrogenase,direct electron transfer,lactose},
  language     = {eng},
  month        = {10},
  number       = {10},
  pages        = {2311--2319},
  publisher    = {John Wiley & Sons},
  series       = {Electroanalysis},
  title        = {Carbon Aerogel as Electrode Material for Improved Direct Electron Transfer in Biosensors Incorporating Cellobiose Dehydrogenase},
  url          = {http://dx.doi.org/10.1002/elan.201600219},
  volume       = {28},
  year         = {2016},
}