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The HBS1L-MYB intergenic interval associated with elevated HbF levels shows characteristics of a distal regulatory region in erythroid cells

Wahlberg, Karin LU ; Jiang, Jie; Rooks, Helen; Jawaid, Kiran; Matsuda, Fumihiko; Yamaguchi, Masao; Lathrop, Mark; Thein, Swee Lay and Best, Steve (2009) In Blood 114(6). p.62-1254
Abstract

HBS1L-MYB intergenic polymorphism (HMIP) on chromosome 6q23 is associated with elevated fetal hemoglobin levels and has pleiotropic effects on several hematologic parameters. To investigate potential regulatory activity in the region, we have measured sensitivity of the sequences to DNase I cleavage that identified 3 tissue-specific DNase I hypersensitive sites in the core intergenic interval. Chromatin immunoprecipitation with microarray (ChIP-chip) analysis showed strong histone acetylation in a defined interval of 65 kb corresponding to the core HBS1L-MYB intergenic region in primary human erythroid cells but not in non-MYB-expressing HeLa cells. ChIP-chip analysis also identified several potential cis-regulatory elements as strong... (More)

HBS1L-MYB intergenic polymorphism (HMIP) on chromosome 6q23 is associated with elevated fetal hemoglobin levels and has pleiotropic effects on several hematologic parameters. To investigate potential regulatory activity in the region, we have measured sensitivity of the sequences to DNase I cleavage that identified 3 tissue-specific DNase I hypersensitive sites in the core intergenic interval. Chromatin immunoprecipitation with microarray (ChIP-chip) analysis showed strong histone acetylation in a defined interval of 65 kb corresponding to the core HBS1L-MYB intergenic region in primary human erythroid cells but not in non-MYB-expressing HeLa cells. ChIP-chip analysis also identified several potential cis-regulatory elements as strong GATA-1 signals that coincided with the DNase I hypersensitive sites present in MYB-expressing erythroid cells. We suggest that HMIP contains regulatory sequences that could be important in hematopoiesis by controlling MYB expression. This study provides the functional link between genetic association of HMIP with control of fetal hemoglobin and other hematologic parameters. We also present a large-scale analysis of histone acetylation as well as RNA polymerase II and GATA-1 interactions on chromosome 6q, and alpha and beta globin gene loci. The data suggest that GATA-1 regulates numerous genes of various functions on chromosome 6q.

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published
keywords
Acetylation, Chromosomes, Human, Pair 6, DNA, Intergenic, Deoxyribonuclease I, Fetal Hemoglobin, GATA1 Transcription Factor, Gene Expression Regulation, Genes, myb, HeLa Cells, Histones, Humans, K562 Cells, Quantitative Trait Loci, Regulatory Elements, Transcriptional, Journal Article, Research Support, Non-U.S. Gov't
in
Blood
volume
114
issue
6
pages
9 pages
publisher
American Society of Hematology
external identifiers
  • scopus:69849101637
ISSN
1528-0020
DOI
10.1182/blood-2009-03-210146
language
English
LU publication?
no
id
74a44596-810f-43d5-96d5-77d490dbcc05
date added to LUP
2017-10-25 11:10:14
date last changed
2017-11-03 10:22:14
@article{74a44596-810f-43d5-96d5-77d490dbcc05,
  abstract     = {<p>HBS1L-MYB intergenic polymorphism (HMIP) on chromosome 6q23 is associated with elevated fetal hemoglobin levels and has pleiotropic effects on several hematologic parameters. To investigate potential regulatory activity in the region, we have measured sensitivity of the sequences to DNase I cleavage that identified 3 tissue-specific DNase I hypersensitive sites in the core intergenic interval. Chromatin immunoprecipitation with microarray (ChIP-chip) analysis showed strong histone acetylation in a defined interval of 65 kb corresponding to the core HBS1L-MYB intergenic region in primary human erythroid cells but not in non-MYB-expressing HeLa cells. ChIP-chip analysis also identified several potential cis-regulatory elements as strong GATA-1 signals that coincided with the DNase I hypersensitive sites present in MYB-expressing erythroid cells. We suggest that HMIP contains regulatory sequences that could be important in hematopoiesis by controlling MYB expression. This study provides the functional link between genetic association of HMIP with control of fetal hemoglobin and other hematologic parameters. We also present a large-scale analysis of histone acetylation as well as RNA polymerase II and GATA-1 interactions on chromosome 6q, and alpha and beta globin gene loci. The data suggest that GATA-1 regulates numerous genes of various functions on chromosome 6q.</p>},
  author       = {Wahlberg, Karin and Jiang, Jie and Rooks, Helen and Jawaid, Kiran and Matsuda, Fumihiko and Yamaguchi, Masao and Lathrop, Mark and Thein, Swee Lay and Best, Steve},
  issn         = {1528-0020},
  keyword      = {Acetylation,Chromosomes, Human, Pair 6,DNA, Intergenic,Deoxyribonuclease I,Fetal Hemoglobin,GATA1 Transcription Factor,Gene Expression Regulation,Genes, myb,HeLa Cells,Histones,Humans,K562 Cells,Quantitative Trait Loci,Regulatory Elements, Transcriptional,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  month        = {08},
  number       = {6},
  pages        = {62--1254},
  publisher    = {American Society of Hematology},
  series       = {Blood},
  title        = {The HBS1L-MYB intergenic interval associated with elevated HbF levels shows characteristics of a distal regulatory region in erythroid cells},
  url          = {http://dx.doi.org/10.1182/blood-2009-03-210146},
  volume       = {114},
  year         = {2009},
}