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Whole cell based microcontact imprinted capacitive biosensor for the detection of Escherichia coli

Idil, Neslihan; Hedström, Martin LU ; Denizli, Adil and Mattiasson, Bo LU (2017) In Biosensors and Bioelectronics 87. p.807-815
Abstract

In this study, a label-free, selective and sensitive microcontact imprinted capacitive biosensor was developed for the detection of Escherichia coli. The recognition of E. coli was successfully performed by this sensor prepared with the combination of microcontact imprinting method and capacitive biosensor technology. After preparation of bacterial stamps, microcontact-E. coli imprinted gold electrodes were generated using an amino acid based recognition element, N-methacryloyl-L-histidine methylester (MAH), 2-Hydroxyethyl methacrylate (HEMA) as monomers and ethyleneglycol dimethacrylate (EGDMA) as crosslinker under UV-polymerization. Real-time E. coli detection experiments were carried out within the range of... (More)

In this study, a label-free, selective and sensitive microcontact imprinted capacitive biosensor was developed for the detection of Escherichia coli. The recognition of E. coli was successfully performed by this sensor prepared with the combination of microcontact imprinting method and capacitive biosensor technology. After preparation of bacterial stamps, microcontact-E. coli imprinted gold electrodes were generated using an amino acid based recognition element, N-methacryloyl-L-histidine methylester (MAH), 2-Hydroxyethyl methacrylate (HEMA) as monomers and ethyleneglycol dimethacrylate (EGDMA) as crosslinker under UV-polymerization. Real-time E. coli detection experiments were carried out within the range of 1.0×102–1.0×107 CFU/mL. The unique combination of these two techniques provides selective detection with a detection limit of 70 CFU/mL. The designed capacitive sensor has high selectivity and was able to distinguish E. coli when present together with competing bacterial strains which are known to have similar shape. In addition, the prepared sensor has the ability to detect E. coli with a recovery of 81–97% in e.g. river water.

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type
Contribution to journal
publication status
published
subject
keywords
Capacitive biosensor, Escherichia coli, Microcontact imprinting, N-methacryloyl-L-histidine methylester
in
Biosensors and Bioelectronics
volume
87
pages
9 pages
publisher
Elsevier
external identifiers
  • scopus:84988027007
ISSN
0956-5663
DOI
10.1016/j.bios.2016.08.096
language
English
LU publication?
yes
id
74afed84-80bf-4c09-9901-6976c284b677
date added to LUP
2016-10-11 10:27:17
date last changed
2017-05-21 04:54:25
@article{74afed84-80bf-4c09-9901-6976c284b677,
  abstract     = {<p>In this study, a label-free, selective and sensitive microcontact imprinted capacitive biosensor was developed for the detection of Escherichia coli. The recognition of E. coli was successfully performed by this sensor prepared with the combination of microcontact imprinting method and capacitive biosensor technology. After preparation of bacterial stamps, microcontact-E. coli imprinted gold electrodes were generated using an amino acid based recognition element, N-methacryloyl-L-histidine methylester (MAH), 2-Hydroxyethyl methacrylate (HEMA) as monomers and ethyleneglycol dimethacrylate (EGDMA) as crosslinker under UV-polymerization. Real-time E. coli detection experiments were carried out within the range of 1.0×10<sup>2</sup>–1.0×10<sup>7</sup> CFU/mL. The unique combination of these two techniques provides selective detection with a detection limit of 70 CFU/mL. The designed capacitive sensor has high selectivity and was able to distinguish E. coli when present together with competing bacterial strains which are known to have similar shape. In addition, the prepared sensor has the ability to detect E. coli with a recovery of 81–97% in e.g. river water.</p>},
  author       = {Idil, Neslihan and Hedström, Martin and Denizli, Adil and Mattiasson, Bo},
  issn         = {0956-5663},
  keyword      = {Capacitive biosensor,Escherichia coli,Microcontact imprinting,N-methacryloyl-L-histidine methylester},
  language     = {eng},
  month        = {01},
  pages        = {807--815},
  publisher    = {Elsevier},
  series       = {Biosensors and Bioelectronics},
  title        = {Whole cell based microcontact imprinted capacitive biosensor for the detection of Escherichia coli},
  url          = {http://dx.doi.org/10.1016/j.bios.2016.08.096},
  volume       = {87},
  year         = {2017},
}