Whole cell based microcontact imprinted capacitive biosensor for the detection of Escherichia coli
(2017) In Biosensors and Bioelectronics 87. p.807-815- Abstract
In this study, a label-free, selective and sensitive microcontact imprinted capacitive biosensor was developed for the detection of Escherichia coli. The recognition of E. coli was successfully performed by this sensor prepared with the combination of microcontact imprinting method and capacitive biosensor technology. After preparation of bacterial stamps, microcontact-E. coli imprinted gold electrodes were generated using an amino acid based recognition element, N-methacryloyl-L-histidine methylester (MAH), 2-Hydroxyethyl methacrylate (HEMA) as monomers and ethyleneglycol dimethacrylate (EGDMA) as crosslinker under UV-polymerization. Real-time E. coli detection experiments were carried out within the range of... (More)
In this study, a label-free, selective and sensitive microcontact imprinted capacitive biosensor was developed for the detection of Escherichia coli. The recognition of E. coli was successfully performed by this sensor prepared with the combination of microcontact imprinting method and capacitive biosensor technology. After preparation of bacterial stamps, microcontact-E. coli imprinted gold electrodes were generated using an amino acid based recognition element, N-methacryloyl-L-histidine methylester (MAH), 2-Hydroxyethyl methacrylate (HEMA) as monomers and ethyleneglycol dimethacrylate (EGDMA) as crosslinker under UV-polymerization. Real-time E. coli detection experiments were carried out within the range of 1.0×102–1.0×107 CFU/mL. The unique combination of these two techniques provides selective detection with a detection limit of 70 CFU/mL. The designed capacitive sensor has high selectivity and was able to distinguish E. coli when present together with competing bacterial strains which are known to have similar shape. In addition, the prepared sensor has the ability to detect E. coli with a recovery of 81–97% in e.g. river water.
(Less)
- author
- Idil, Neslihan ; Hedström, Martin LU ; Denizli, Adil and Mattiasson, Bo LU
- organization
- publishing date
- 2017-01-15
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Capacitive biosensor, Escherichia coli, Microcontact imprinting, N-methacryloyl-L-histidine methylester
- in
- Biosensors and Bioelectronics
- volume
- 87
- pages
- 9 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:84988027007
- pmid:27657842
- wos:000390499600111
- ISSN
- 0956-5663
- DOI
- 10.1016/j.bios.2016.08.096
- language
- English
- LU publication?
- yes
- id
- 74afed84-80bf-4c09-9901-6976c284b677
- date added to LUP
- 2016-10-11 10:27:17
- date last changed
- 2024-04-19 11:14:24
@article{74afed84-80bf-4c09-9901-6976c284b677,
abstract = {{<p>In this study, a label-free, selective and sensitive microcontact imprinted capacitive biosensor was developed for the detection of Escherichia coli. The recognition of E. coli was successfully performed by this sensor prepared with the combination of microcontact imprinting method and capacitive biosensor technology. After preparation of bacterial stamps, microcontact-E. coli imprinted gold electrodes were generated using an amino acid based recognition element, N-methacryloyl-L-histidine methylester (MAH), 2-Hydroxyethyl methacrylate (HEMA) as monomers and ethyleneglycol dimethacrylate (EGDMA) as crosslinker under UV-polymerization. Real-time E. coli detection experiments were carried out within the range of 1.0×10<sup>2</sup>–1.0×10<sup>7</sup> CFU/mL. The unique combination of these two techniques provides selective detection with a detection limit of 70 CFU/mL. The designed capacitive sensor has high selectivity and was able to distinguish E. coli when present together with competing bacterial strains which are known to have similar shape. In addition, the prepared sensor has the ability to detect E. coli with a recovery of 81–97% in e.g. river water.</p>}},
author = {{Idil, Neslihan and Hedström, Martin and Denizli, Adil and Mattiasson, Bo}},
issn = {{0956-5663}},
keywords = {{Capacitive biosensor; Escherichia coli; Microcontact imprinting; N-methacryloyl-L-histidine methylester}},
language = {{eng}},
month = {{01}},
pages = {{807--815}},
publisher = {{Elsevier}},
series = {{Biosensors and Bioelectronics}},
title = {{Whole cell based microcontact imprinted capacitive biosensor for the detection of Escherichia coli}},
url = {{http://dx.doi.org/10.1016/j.bios.2016.08.096}},
doi = {{10.1016/j.bios.2016.08.096}},
volume = {{87}},
year = {{2017}},
}