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Scalable, high performance, enzymatic cathodes based on nanoimprint lithography

Pankratov, Dmitry; Sundberg, Richard LU ; Sotres, Javier; Suyatin, Dmitry LU ; Maximov, Ivan LU ; Shleev, Sergey and Montelius, Lars LU (2015) In Beilstein Journal of Nanotechnology 6. p.1377-1384
Abstract
Here we detail high performance, enzymatic electrodes for oxygen bio-electroreduction, which can be easily and reproducibly fabricated with industry-scale throughput. Planar and nanostructured electrodes were built on biocompatible, flexible polymer sheets, while nanoimprint lithography was used for electrode nanostructuring. To the best of our knowledge, this is one of the first reports concerning the usage of nanoimprint lithography for amperometric bioelectronic devices. The enzyme (Myrothecium verrucaria bilirubin oxidase) was immobilised on planar (control) and artificially nanostructured, gold electrodes by direct physical adsorption. The detailed electrochemical investigation of bioelectrodes was performed and the following... (More)
Here we detail high performance, enzymatic electrodes for oxygen bio-electroreduction, which can be easily and reproducibly fabricated with industry-scale throughput. Planar and nanostructured electrodes were built on biocompatible, flexible polymer sheets, while nanoimprint lithography was used for electrode nanostructuring. To the best of our knowledge, this is one of the first reports concerning the usage of nanoimprint lithography for amperometric bioelectronic devices. The enzyme (Myrothecium verrucaria bilirubin oxidase) was immobilised on planar (control) and artificially nanostructured, gold electrodes by direct physical adsorption. The detailed electrochemical investigation of bioelectrodes was performed and the following parameters were obtained: open circuit voltage of approximately 0.75 V, and maximum bio-electrocatalytic current densities of 18 mu A/cm(2) and 58 mu A/cm(2) in air-saturated buffers versus 48 mu A/cm(2) and 186 mu A/cm(2) in oxygen-saturated buffers for planar and nanostructured electrodes, respectively. The half-deactivation times of planar and nanostructured biocathodes were measured to be 2 h and 14 h, respectively. The comparison of standard heterogeneous and bio-electrocatalytic rate constants showed that the improved bio-electrocatalytic performance of the nanostructured biocathodes compared to planar biodevices is due to the increased surface area of the nanostructured electrodes, whereas their improved operational stability is attributed to stabilisation of the enzyme inside nanocavities. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
bilirubin oxidase, bio-electrocatalysis, direct electron transfer, nanoimprint lithography, oxygen reduction reaction
in
Beilstein Journal of Nanotechnology
volume
6
pages
1377 - 1384
publisher
Beilstein-Institut
external identifiers
  • wos:000356588300001
  • scopus:84933047555
  • pmid:26199841
ISSN
2190-4286
DOI
10.3762/bjnano.6.142
language
English
LU publication?
yes
id
10cbe2a1-bb5e-43c1-9d51-5412b9c8a29f (old id 7596815)
date added to LUP
2015-07-23 11:26:27
date last changed
2017-01-01 06:25:59
@article{10cbe2a1-bb5e-43c1-9d51-5412b9c8a29f,
  abstract     = {Here we detail high performance, enzymatic electrodes for oxygen bio-electroreduction, which can be easily and reproducibly fabricated with industry-scale throughput. Planar and nanostructured electrodes were built on biocompatible, flexible polymer sheets, while nanoimprint lithography was used for electrode nanostructuring. To the best of our knowledge, this is one of the first reports concerning the usage of nanoimprint lithography for amperometric bioelectronic devices. The enzyme (Myrothecium verrucaria bilirubin oxidase) was immobilised on planar (control) and artificially nanostructured, gold electrodes by direct physical adsorption. The detailed electrochemical investigation of bioelectrodes was performed and the following parameters were obtained: open circuit voltage of approximately 0.75 V, and maximum bio-electrocatalytic current densities of 18 mu A/cm(2) and 58 mu A/cm(2) in air-saturated buffers versus 48 mu A/cm(2) and 186 mu A/cm(2) in oxygen-saturated buffers for planar and nanostructured electrodes, respectively. The half-deactivation times of planar and nanostructured biocathodes were measured to be 2 h and 14 h, respectively. The comparison of standard heterogeneous and bio-electrocatalytic rate constants showed that the improved bio-electrocatalytic performance of the nanostructured biocathodes compared to planar biodevices is due to the increased surface area of the nanostructured electrodes, whereas their improved operational stability is attributed to stabilisation of the enzyme inside nanocavities.},
  author       = {Pankratov, Dmitry and Sundberg, Richard and Sotres, Javier and Suyatin, Dmitry and Maximov, Ivan and Shleev, Sergey and Montelius, Lars},
  issn         = {2190-4286},
  keyword      = {bilirubin oxidase,bio-electrocatalysis,direct electron transfer,nanoimprint lithography,oxygen reduction reaction},
  language     = {eng},
  pages        = {1377--1384},
  publisher    = {Beilstein-Institut},
  series       = {Beilstein Journal of Nanotechnology},
  title        = {Scalable, high performance, enzymatic cathodes based on nanoimprint lithography},
  url          = {http://dx.doi.org/10.3762/bjnano.6.142},
  volume       = {6},
  year         = {2015},
}