Scalable, high performance, enzymatic cathodes based on nanoimprint lithography
Pankratov, Dmitry ; Sundberg, Richard LU ; Sotres, Javier ; Suyatin, Dmitry LU
; Maximov, Ivan
LU
; Shleev, Sergey
and Montelius, Lars
LU
(2015)
In Beilstein Journal of Nanotechnology
6.
p.1377-1384
- Abstract
- Here we detail high performance, enzymatic electrodes for oxygen bio-electroreduction, which can be easily and reproducibly fabricated with industry-scale throughput. Planar and nanostructured electrodes were built on biocompatible, flexible polymer sheets, while nanoimprint lithography was used for electrode nanostructuring. To the best of our knowledge, this is one of the first reports concerning the usage of nanoimprint lithography for amperometric bioelectronic devices. The enzyme (Myrothecium verrucaria bilirubin oxidase) was immobilised on planar (control) and artificially nanostructured, gold electrodes by direct physical adsorption. The detailed electrochemical investigation of bioelectrodes was performed and the following... (More)
- Here we detail high performance, enzymatic electrodes for oxygen bio-electroreduction, which can be easily and reproducibly fabricated with industry-scale throughput. Planar and nanostructured electrodes were built on biocompatible, flexible polymer sheets, while nanoimprint lithography was used for electrode nanostructuring. To the best of our knowledge, this is one of the first reports concerning the usage of nanoimprint lithography for amperometric bioelectronic devices. The enzyme (Myrothecium verrucaria bilirubin oxidase) was immobilised on planar (control) and artificially nanostructured, gold electrodes by direct physical adsorption. The detailed electrochemical investigation of bioelectrodes was performed and the following parameters were obtained: open circuit voltage of approximately 0.75 V, and maximum bio-electrocatalytic current densities of 18 mu A/cm(2) and 58 mu A/cm(2) in air-saturated buffers versus 48 mu A/cm(2) and 186 mu A/cm(2) in oxygen-saturated buffers for planar and nanostructured electrodes, respectively. The half-deactivation times of planar and nanostructured biocathodes were measured to be 2 h and 14 h, respectively. The comparison of standard heterogeneous and bio-electrocatalytic rate constants showed that the improved bio-electrocatalytic performance of the nanostructured biocathodes compared to planar biodevices is due to the increased surface area of the nanostructured electrodes, whereas their improved operational stability is attributed to stabilisation of the enzyme inside nanocavities. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/7596815
- author
- Pankratov, Dmitry
; Sundberg, Richard
LU
; Sotres, Javier
; Suyatin, Dmitry
LU
; Maximov, Ivan
LU
; Shleev, Sergey
and Montelius, Lars
LU
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- bilirubin oxidase, bio-electrocatalysis, direct electron transfer, nanoimprint lithography, oxygen reduction reaction
- in
- Beilstein Journal of Nanotechnology
- volume
- 6
- pages
- 1377 - 1384
- publisher
- Beilstein-Institut
- external identifiers
-
- wos:000356588300001
- scopus:84933047555
- pmid:26199841
- pmid:26199841
- ISSN
- 2190-4286
- DOI
- 10.3762/bjnano.6.142
- language
- English
- LU publication?
- yes
- id
- 10cbe2a1-bb5e-43c1-9d51-5412b9c8a29f (old id 7596815)
- date added to LUP
- 2016-04-01 14:47:14
- date last changed
- 2023-09-03 19:14:07
@article{10cbe2a1-bb5e-43c1-9d51-5412b9c8a29f,
abstract = {{Here we detail high performance, enzymatic electrodes for oxygen bio-electroreduction, which can be easily and reproducibly fabricated with industry-scale throughput. Planar and nanostructured electrodes were built on biocompatible, flexible polymer sheets, while nanoimprint lithography was used for electrode nanostructuring. To the best of our knowledge, this is one of the first reports concerning the usage of nanoimprint lithography for amperometric bioelectronic devices. The enzyme (Myrothecium verrucaria bilirubin oxidase) was immobilised on planar (control) and artificially nanostructured, gold electrodes by direct physical adsorption. The detailed electrochemical investigation of bioelectrodes was performed and the following parameters were obtained: open circuit voltage of approximately 0.75 V, and maximum bio-electrocatalytic current densities of 18 mu A/cm(2) and 58 mu A/cm(2) in air-saturated buffers versus 48 mu A/cm(2) and 186 mu A/cm(2) in oxygen-saturated buffers for planar and nanostructured electrodes, respectively. The half-deactivation times of planar and nanostructured biocathodes were measured to be 2 h and 14 h, respectively. The comparison of standard heterogeneous and bio-electrocatalytic rate constants showed that the improved bio-electrocatalytic performance of the nanostructured biocathodes compared to planar biodevices is due to the increased surface area of the nanostructured electrodes, whereas their improved operational stability is attributed to stabilisation of the enzyme inside nanocavities.}},
author = {{Pankratov, Dmitry and Sundberg, Richard and Sotres, Javier and Suyatin, Dmitry and Maximov, Ivan and Shleev, Sergey and Montelius, Lars}},
issn = {{2190-4286}},
keywords = {{bilirubin oxidase; bio-electrocatalysis; direct electron transfer; nanoimprint lithography; oxygen reduction reaction}},
language = {{eng}},
pages = {{1377--1384}},
publisher = {{Beilstein-Institut}},
series = {{Beilstein Journal of Nanotechnology}},
title = {{Scalable, high performance, enzymatic cathodes based on nanoimprint lithography}},
url = {{http://dx.doi.org/10.3762/bjnano.6.142}},
doi = {{10.3762/bjnano.6.142}},
volume = {{6}},
year = {{2015}},
}