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Bacillus subtilis CtaA and CtaB function in haem A biosynthesis

Svensson, Birgitta; Lübben, Mattias and Hederstedt, Lars LU (1993) In Molecular Microbiology 10(1). p.193-201
Abstract
Haem A, a prosthetic group of many respiratory oxidases, is probably synthesized from haem B (protohaem IX) in a pathway in which haem 0 is an intermediate. Possible roles of the Bacillus subtilis ctaA and ctaB gene products in haem 0 and haem A synthesis were studied. Escherichia coli does not contain haem A. The ctaA gene on plasmids in E. coli resulted in haem A accumulation in membranes. The presence of ctaB together with ctaA increased the amount of haem A found in E. coli. Haem 0 was not detected in wild-type B. subtilis strains. A previously isolated B. subtilis ctaA deletion mutant was found to contain haem B and haem 0, but not haem A. B. subtilis ctaB deletion mutants were constructed and found to lack both haem A and haem 0. The... (More)
Haem A, a prosthetic group of many respiratory oxidases, is probably synthesized from haem B (protohaem IX) in a pathway in which haem 0 is an intermediate. Possible roles of the Bacillus subtilis ctaA and ctaB gene products in haem 0 and haem A synthesis were studied. Escherichia coli does not contain haem A. The ctaA gene on plasmids in E. coli resulted in haem A accumulation in membranes. The presence of ctaB together with ctaA increased the amount of haem A found in E. coli. Haem 0 was not detected in wild-type B. subtilis strains. A previously isolated B. subtilis ctaA deletion mutant was found to contain haem B and haem 0, but not haem A. B. subtilis ctaB deletion mutants were constructed and found to lack both haem A and haem 0. The results with E. coli and B. subtilis strongly suggest that the B. subtilis CtaA protein functions in haem A synthesis. It is tentatively suggested that it functions in the oxygenation/oxidation of the methyl side group of carbon 8 of haem 0. B. subtilis CtaB, which is homologous to Saccharomyces cerevisiae COX10 and E. coli CyoE, also has a role in haem A synthesis and seems to be required for both cytochrome a and cytochrome o synthesis. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular Microbiology
volume
10
issue
1
pages
193 - 201
publisher
Wiley-Blackwell
external identifiers
  • scopus:0027527547
ISSN
1365-2958
DOI
10.1111/j.1365-2958.1993.tb00915.x
language
English
LU publication?
yes
id
75a28bdb-5356-4974-87ee-e8fae9b738d9
date added to LUP
2017-07-18 10:20:24
date last changed
2017-08-27 06:44:47
@article{75a28bdb-5356-4974-87ee-e8fae9b738d9,
  abstract     = {Haem A, a prosthetic group of many respiratory oxidases, is probably synthesized from haem B (protohaem IX) in a pathway in which haem 0 is an intermediate. Possible roles of the Bacillus subtilis ctaA and ctaB gene products in haem 0 and haem A synthesis were studied. Escherichia coli does not contain haem A. The ctaA gene on plasmids in E. coli resulted in haem A accumulation in membranes. The presence of ctaB together with ctaA increased the amount of haem A found in E. coli. Haem 0 was not detected in wild-type B. subtilis strains. A previously isolated B. subtilis ctaA deletion mutant was found to contain haem B and haem 0, but not haem A. B. subtilis ctaB deletion mutants were constructed and found to lack both haem A and haem 0. The results with E. coli and B. subtilis strongly suggest that the B. subtilis CtaA protein functions in haem A synthesis. It is tentatively suggested that it functions in the oxygenation/oxidation of the methyl side group of carbon 8 of haem 0. B. subtilis CtaB, which is homologous to Saccharomyces cerevisiae COX10 and E. coli CyoE, also has a role in haem A synthesis and seems to be required for both cytochrome a and cytochrome o synthesis.},
  author       = {Svensson, Birgitta and Lübben, Mattias and Hederstedt, Lars},
  issn         = {1365-2958},
  language     = {eng},
  number       = {1},
  pages        = {193--201},
  publisher    = {Wiley-Blackwell},
  series       = {Molecular Microbiology},
  title        = {<em>Bacillus subtilis</em> CtaA and CtaB function in haem A biosynthesis},
  url          = {http://dx.doi.org/10.1111/j.1365-2958.1993.tb00915.x },
  volume       = {10},
  year         = {1993},
}