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Cerebrovascular endothelin-1 hyper-reactivity is associated with transient receptor potential canonical channels 1 and 6 activation and delayed cerebral hypoperfusion after forebrain ischaemia in rats

Johansson, S. E.; Andersen, X. E. D. R.; Hansen, R. H.; Povlsen, G. K. and Edvinsson, Lars LU (2015) In Acta Physiologica 214(3). p.376-389
Abstract
Aim: In this study, we aimed to investigate whether changes in cerebrovascular voltage-dependent calcium channels and non-selective cation channels contribute to the enhanced endothelin-1-mediated vasoconstriction in the delayed hypoperfusion phase after experimental transient forebrain ischaemia. Methods: Experimental forebrain ischaemia was induced in Wistar male rats by a two-vessel occlusion model, and the cerebral blood flow was measured by magnetic resonance imaging two days after reperfusion. In vitro vasoreactivity studies, immunofluorescence and quantitative PCR were performed on cerebral arteries from ischaemic or sham-operated rats to evaluate changes in vascular voltage-dependent calcium channels, transient receptor potential... (More)
Aim: In this study, we aimed to investigate whether changes in cerebrovascular voltage-dependent calcium channels and non-selective cation channels contribute to the enhanced endothelin-1-mediated vasoconstriction in the delayed hypoperfusion phase after experimental transient forebrain ischaemia. Methods: Experimental forebrain ischaemia was induced in Wistar male rats by a two-vessel occlusion model, and the cerebral blood flow was measured by magnetic resonance imaging two days after reperfusion. In vitro vasoreactivity studies, immunofluorescence and quantitative PCR were performed on cerebral arteries from ischaemic or sham-operated rats to evaluate changes in vascular voltage-dependent calcium channels, transient receptor potential canonical channels as well as endothelin-1 receptor function and expression. Results: The expression of transient receptor potential canonical channels 1 and 6 in the vascular smooth muscle cells was enhanced and correlated with decreased cerebral blood flow two days after forebrain ischaemia. Furthermore, under conditions when voltage-dependent calcium channels were inhibited, endothelin-1-induced cerebrovascular contraction was enhanced and this enhancement was presumably mediated by Ca2+ influx via upregulated transient receptor potential canonical channels 1 and 6. Conclusions: Our data demonstrates that endothelin-1-mediated influx of extracellular Ca2+ activates transient receptor potential canonical channels 1 and 6 in cerebral vascular smooth muscle cells. This seems to have an important role in the enhanced cerebral vasoconstriction in the delayed post-ischaemic hypoperfusion phase after experimental forebrain ischaemia. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cerebral arteries, endothelin-1, global cerebral ischaemia, hypoperfusion, transient receptor potential canonical channels
in
Acta Physiologica
volume
214
issue
3
pages
376 - 389
publisher
Wiley-Blackwell
external identifiers
  • wos:000356306300012
  • scopus:84930886225
ISSN
1748-1708
DOI
10.1111/apha.12519
language
English
LU publication?
yes
id
489447ec-7791-4acf-a823-40b744cdfd13 (old id 7602032)
date added to LUP
2015-08-03 10:08:17
date last changed
2017-08-13 03:09:34
@article{489447ec-7791-4acf-a823-40b744cdfd13,
  abstract     = {Aim: In this study, we aimed to investigate whether changes in cerebrovascular voltage-dependent calcium channels and non-selective cation channels contribute to the enhanced endothelin-1-mediated vasoconstriction in the delayed hypoperfusion phase after experimental transient forebrain ischaemia. Methods: Experimental forebrain ischaemia was induced in Wistar male rats by a two-vessel occlusion model, and the cerebral blood flow was measured by magnetic resonance imaging two days after reperfusion. In vitro vasoreactivity studies, immunofluorescence and quantitative PCR were performed on cerebral arteries from ischaemic or sham-operated rats to evaluate changes in vascular voltage-dependent calcium channels, transient receptor potential canonical channels as well as endothelin-1 receptor function and expression. Results: The expression of transient receptor potential canonical channels 1 and 6 in the vascular smooth muscle cells was enhanced and correlated with decreased cerebral blood flow two days after forebrain ischaemia. Furthermore, under conditions when voltage-dependent calcium channels were inhibited, endothelin-1-induced cerebrovascular contraction was enhanced and this enhancement was presumably mediated by Ca2+ influx via upregulated transient receptor potential canonical channels 1 and 6. Conclusions: Our data demonstrates that endothelin-1-mediated influx of extracellular Ca2+ activates transient receptor potential canonical channels 1 and 6 in cerebral vascular smooth muscle cells. This seems to have an important role in the enhanced cerebral vasoconstriction in the delayed post-ischaemic hypoperfusion phase after experimental forebrain ischaemia.},
  author       = {Johansson, S. E. and Andersen, X. E. D. R. and Hansen, R. H. and Povlsen, G. K. and Edvinsson, Lars},
  issn         = {1748-1708},
  keyword      = {cerebral arteries,endothelin-1,global cerebral ischaemia,hypoperfusion,transient receptor potential canonical channels},
  language     = {eng},
  number       = {3},
  pages        = {376--389},
  publisher    = {Wiley-Blackwell},
  series       = {Acta Physiologica},
  title        = {Cerebrovascular endothelin-1 hyper-reactivity is associated with transient receptor potential canonical channels 1 and 6 activation and delayed cerebral hypoperfusion after forebrain ischaemia in rats},
  url          = {http://dx.doi.org/10.1111/apha.12519},
  volume       = {214},
  year         = {2015},
}