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Histologic distribution and biochemical properties of alpha 1-microglobulin in human placenta

Berggård, T LU ; Enghild, J J ; Badve, S ; Salafia, C M ; Lögdberg, L and Akerström, B LU (1999) In American Journal of Reproductive Immunology and Microbiology 41(1). p.52-60
Abstract

PROBLEM: The embryo is protected from immunologic rejection by the mother, possibly accomplished by immunosuppressive molecules located in the placenta. We investigated the distribution and biochemical properties in placenta of the immunosuppressive plasma protein alpha 1-microglobulin.

METHOD OF STUDY: Placental alpha 1-microglobulin was investigated by immunohistochemistry and, after extraction, by electrophoresis, immunoblotting and radioimmunoassay.

RESULTS: alpha 1-Microglobulin staining was observed in the intervillous fibrin and in syncytiotrophoblasts, especially at sites with syncytial injury. Strongly stained single cells in the intervillous spaces and variably stained intravillous histiocytes were noted.... (More)

PROBLEM: The embryo is protected from immunologic rejection by the mother, possibly accomplished by immunosuppressive molecules located in the placenta. We investigated the distribution and biochemical properties in placenta of the immunosuppressive plasma protein alpha 1-microglobulin.

METHOD OF STUDY: Placental alpha 1-microglobulin was investigated by immunohistochemistry and, after extraction, by electrophoresis, immunoblotting and radioimmunoassay.

RESULTS: alpha 1-Microglobulin staining was observed in the intervillous fibrin and in syncytiotrophoblasts, especially at sites with syncytial injury. Strongly stained single cells in the intervillous spaces and variably stained intravillous histiocytes were noted. Solubilization of the placenta-matrix fraction and placenta membrane fraction released predominantly the free form of alpha 1-microglobulin, but, additionally, an apparently truncated form from the placenta-membrane fraction. The soluble fraction of placenta contained two novel alpha 1-microglobulin complexes.

CONCLUSIONS: The biochemical analysis indicates the presence in placenta of alpha 1-microglobulin forms not found in blood. The histochemical analysis supports the possibility that alpha 1-microglobulin may function as a local immunoregulator in the placenta.

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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
Electrophoresis, Polyacrylamide Gel, Female, Humans, Immunoblotting, Immunohistochemistry, Placenta/chemistry, Pregnancy, Radioimmunoassay, beta 2-Microglobulin/analysis
in
American Journal of Reproductive Immunology and Microbiology
volume
41
issue
1
pages
52 - 60
publisher
Wiley-Blackwell
external identifiers
  • pmid:10097787
  • scopus:0345059886
ISSN
1046-7408
DOI
10.1111/j.1600-0897.1999.tb00075.x
language
English
LU publication?
yes
id
76112653-71dd-42a2-86e0-0a0995e7607d
date added to LUP
2019-05-22 10:10:27
date last changed
2020-01-16 03:55:58
@article{76112653-71dd-42a2-86e0-0a0995e7607d,
  abstract     = {<p>PROBLEM: The embryo is protected from immunologic rejection by the mother, possibly accomplished by immunosuppressive molecules located in the placenta. We investigated the distribution and biochemical properties in placenta of the immunosuppressive plasma protein alpha 1-microglobulin.</p><p>METHOD OF STUDY: Placental alpha 1-microglobulin was investigated by immunohistochemistry and, after extraction, by electrophoresis, immunoblotting and radioimmunoassay.</p><p>RESULTS: alpha 1-Microglobulin staining was observed in the intervillous fibrin and in syncytiotrophoblasts, especially at sites with syncytial injury. Strongly stained single cells in the intervillous spaces and variably stained intravillous histiocytes were noted. Solubilization of the placenta-matrix fraction and placenta membrane fraction released predominantly the free form of alpha 1-microglobulin, but, additionally, an apparently truncated form from the placenta-membrane fraction. The soluble fraction of placenta contained two novel alpha 1-microglobulin complexes.</p><p>CONCLUSIONS: The biochemical analysis indicates the presence in placenta of alpha 1-microglobulin forms not found in blood. The histochemical analysis supports the possibility that alpha 1-microglobulin may function as a local immunoregulator in the placenta.</p>},
  author       = {Berggård, T and Enghild, J J and Badve, S and Salafia, C M and Lögdberg, L and Akerström, B},
  issn         = {1046-7408},
  language     = {eng},
  number       = {1},
  pages        = {52--60},
  publisher    = {Wiley-Blackwell},
  series       = {American Journal of Reproductive Immunology and Microbiology},
  title        = {Histologic distribution and biochemical properties of alpha 1-microglobulin in human placenta},
  url          = {http://dx.doi.org/10.1111/j.1600-0897.1999.tb00075.x},
  doi          = {10.1111/j.1600-0897.1999.tb00075.x},
  volume       = {41},
  year         = {1999},
}