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Supported Lipid Bilayer Formation and Lipid-Membrane-Mediated Biorecognition Reactions Studied with a New Nanoplasmonic Sensor Template

Jonsson, Magnus LU ; Jönsson, Peter LU ; Dahlin, Andreas LU and Höök, Fredrik LU (2007) In Nano Letters 7(11). p.3462-3468
Abstract
This paper presents the use of the localized surface plasmon resonance (LSPR) sensor concept to probe the formation of macroscopic and

laterally mobile supported lipid bilayers (SLBs) on SiOx-encapsulated nanohole-containing Au and Ag films. A comparison between Au- and

Ag-based sensor templates demonstrates a higher sensitivity for Au-based templates with respect to both bulk and interfacial refractive index

(RI) changes in aqueous solution. The lateral mobility of SLBs formed on the SiOx-encapsulated nanohole templates was analyzed using

fluorescence recovery after photobleaching (FRAP), demonstrating essentially complete (>96%) recovery, but a reduction in diffusivity of

about 35% compared... (More)
This paper presents the use of the localized surface plasmon resonance (LSPR) sensor concept to probe the formation of macroscopic and

laterally mobile supported lipid bilayers (SLBs) on SiOx-encapsulated nanohole-containing Au and Ag films. A comparison between Au- and

Ag-based sensor templates demonstrates a higher sensitivity for Au-based templates with respect to both bulk and interfacial refractive index

(RI) changes in aqueous solution. The lateral mobility of SLBs formed on the SiOx-encapsulated nanohole templates was analyzed using

fluorescence recovery after photobleaching (FRAP), demonstrating essentially complete (>96%) recovery, but a reduction in diffusivity of

about 35% compared with SLBs formed on flat SiOx substrates. Furthermore, upon SLB formation, the temporal variation in extinction peak

position of the LSPR active templates display a characteristic shape, illustrating what, to the best of our knowledge, is the first example where

the nanoplasmonic concept is shown capable of probing biomacromolecular structural changes without the introduction of labels. With a

signal-to-noise ratio better than 500 upon protein binding to the cell-membrane mimics, the sensor concept is also proven competitive with

state-of-the-art label-free sensors. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Localized Surface Plasmon Resonance Nanoplasmonics Lipid Bilayer Nanosensor Self-Assembly
in
Nano Letters
volume
7
issue
11
pages
3462 - 3468
publisher
The American Chemical Society
external identifiers
  • WOS:000251059800038
  • Scopus:36749023298
ISSN
1530-6992
DOI
10.1021/nl072006t
language
English
LU publication?
yes
id
a3cb1569-53bf-4b0d-8868-9cfe20d13894 (old id 764049)
alternative location
http://pubs.acs.org/cgi-bin/abstract.cgi/nalefd/2007/7/i11/abs/nl072006t.html
date added to LUP
2007-12-17 18:04:09
date last changed
2017-02-12 04:31:09
@article{a3cb1569-53bf-4b0d-8868-9cfe20d13894,
  abstract     = {This paper presents the use of the localized surface plasmon resonance (LSPR) sensor concept to probe the formation of macroscopic and<br/><br>
laterally mobile supported lipid bilayers (SLBs) on SiOx-encapsulated nanohole-containing Au and Ag films. A comparison between Au- and<br/><br>
Ag-based sensor templates demonstrates a higher sensitivity for Au-based templates with respect to both bulk and interfacial refractive index<br/><br>
(RI) changes in aqueous solution. The lateral mobility of SLBs formed on the SiOx-encapsulated nanohole templates was analyzed using<br/><br>
fluorescence recovery after photobleaching (FRAP), demonstrating essentially complete (&gt;96%) recovery, but a reduction in diffusivity of<br/><br>
about 35% compared with SLBs formed on flat SiOx substrates. Furthermore, upon SLB formation, the temporal variation in extinction peak<br/><br>
position of the LSPR active templates display a characteristic shape, illustrating what, to the best of our knowledge, is the first example where<br/><br>
the nanoplasmonic concept is shown capable of probing biomacromolecular structural changes without the introduction of labels. With a<br/><br>
signal-to-noise ratio better than 500 upon protein binding to the cell-membrane mimics, the sensor concept is also proven competitive with<br/><br>
state-of-the-art label-free sensors.},
  author       = {Jonsson, Magnus and Jönsson, Peter and Dahlin, Andreas and Höök, Fredrik},
  issn         = {1530-6992},
  keyword      = {Localized Surface Plasmon Resonance
Nanoplasmonics
Lipid Bilayer
Nanosensor
Self-Assembly},
  language     = {eng},
  number       = {11},
  pages        = {3462--3468},
  publisher    = {The American Chemical Society},
  series       = {Nano Letters},
  title        = {Supported Lipid Bilayer Formation and Lipid-Membrane-Mediated Biorecognition Reactions Studied with a New Nanoplasmonic Sensor Template},
  url          = {http://dx.doi.org/10.1021/nl072006t},
  volume       = {7},
  year         = {2007},
}