Supported Lipid Bilayer Formation and Lipid-Membrane-Mediated Biorecognition Reactions Studied with a New Nanoplasmonic Sensor Template
Jonsson, Magnus LU ; Jönsson, Peter LU
; Dahlin, Andreas
LU
and Höök, Fredrik
LU
(2007)
In Nano Letters
7(11).
p.3462-3468
- Abstract
- This paper presents the use of the localized surface plasmon resonance (LSPR) sensor concept to probe the formation of macroscopic and
laterally mobile supported lipid bilayers (SLBs) on SiOx-encapsulated nanohole-containing Au and Ag films. A comparison between Au- and
Ag-based sensor templates demonstrates a higher sensitivity for Au-based templates with respect to both bulk and interfacial refractive index
(RI) changes in aqueous solution. The lateral mobility of SLBs formed on the SiOx-encapsulated nanohole templates was analyzed using
fluorescence recovery after photobleaching (FRAP), demonstrating essentially complete (>96%) recovery, but a reduction in diffusivity of
about 35% compared... (More) - This paper presents the use of the localized surface plasmon resonance (LSPR) sensor concept to probe the formation of macroscopic and
laterally mobile supported lipid bilayers (SLBs) on SiOx-encapsulated nanohole-containing Au and Ag films. A comparison between Au- and
Ag-based sensor templates demonstrates a higher sensitivity for Au-based templates with respect to both bulk and interfacial refractive index
(RI) changes in aqueous solution. The lateral mobility of SLBs formed on the SiOx-encapsulated nanohole templates was analyzed using
fluorescence recovery after photobleaching (FRAP), demonstrating essentially complete (>96%) recovery, but a reduction in diffusivity of
about 35% compared with SLBs formed on flat SiOx substrates. Furthermore, upon SLB formation, the temporal variation in extinction peak
position of the LSPR active templates display a characteristic shape, illustrating what, to the best of our knowledge, is the first example where
the nanoplasmonic concept is shown capable of probing biomacromolecular structural changes without the introduction of labels. With a
signal-to-noise ratio better than 500 upon protein binding to the cell-membrane mimics, the sensor concept is also proven competitive with
state-of-the-art label-free sensors. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/764049
- author
- Jonsson, Magnus
LU
; Jönsson, Peter
LU
; Dahlin, Andreas
LU
and Höök, Fredrik
LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Localized Surface Plasmon Resonance Nanoplasmonics Lipid Bilayer Nanosensor Self-Assembly
- in
- Nano Letters
- volume
- 7
- issue
- 11
- pages
- 3462 - 3468
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000251059800038
- scopus:36749023298
- pmid:17902726
- ISSN
- 1530-6992
- DOI
- 10.1021/nl072006t
- language
- English
- LU publication?
- yes
- id
- a3cb1569-53bf-4b0d-8868-9cfe20d13894 (old id 764049)
- alternative location
- http://pubs.acs.org/cgi-bin/abstract.cgi/nalefd/2007/7/i11/abs/nl072006t.html
- date added to LUP
- 2016-04-04 14:36:45
- date last changed
- 2024-10-05 14:36:19
@article{a3cb1569-53bf-4b0d-8868-9cfe20d13894,
abstract = {{This paper presents the use of the localized surface plasmon resonance (LSPR) sensor concept to probe the formation of macroscopic and<br/><br>
laterally mobile supported lipid bilayers (SLBs) on SiOx-encapsulated nanohole-containing Au and Ag films. A comparison between Au- and<br/><br>
Ag-based sensor templates demonstrates a higher sensitivity for Au-based templates with respect to both bulk and interfacial refractive index<br/><br>
(RI) changes in aqueous solution. The lateral mobility of SLBs formed on the SiOx-encapsulated nanohole templates was analyzed using<br/><br>
fluorescence recovery after photobleaching (FRAP), demonstrating essentially complete (>96%) recovery, but a reduction in diffusivity of<br/><br>
about 35% compared with SLBs formed on flat SiOx substrates. Furthermore, upon SLB formation, the temporal variation in extinction peak<br/><br>
position of the LSPR active templates display a characteristic shape, illustrating what, to the best of our knowledge, is the first example where<br/><br>
the nanoplasmonic concept is shown capable of probing biomacromolecular structural changes without the introduction of labels. With a<br/><br>
signal-to-noise ratio better than 500 upon protein binding to the cell-membrane mimics, the sensor concept is also proven competitive with<br/><br>
state-of-the-art label-free sensors.}},
author = {{Jonsson, Magnus and Jönsson, Peter and Dahlin, Andreas and Höök, Fredrik}},
issn = {{1530-6992}},
keywords = {{Localized Surface Plasmon Resonance
Nanoplasmonics
Lipid Bilayer
Nanosensor
Self-Assembly}},
language = {{eng}},
number = {{11}},
pages = {{3462--3468}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Nano Letters}},
title = {{Supported Lipid Bilayer Formation and Lipid-Membrane-Mediated Biorecognition Reactions Studied with a New Nanoplasmonic Sensor Template}},
url = {{http://dx.doi.org/10.1021/nl072006t}},
doi = {{10.1021/nl072006t}},
volume = {{7}},
year = {{2007}},
}