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Long-term consequences of bilateral cavernous crush injury in normal and diabetic rats : a functional study

Castiglione, Fabio ; Albersen, Maarten ; Fiorenzo, Salvatore ; Hedlund, Petter LU ; Cakir, Omer Onur ; Pavone, Carlo ; Alnajjar, Hussain M. ; Joniau, Steven ; Muneer, Asif and Kluth, Luis A. , et al. (2022) In International Journal of Impotence Research 34(8). p.781-785
Abstract

A recent statement from the European-Society-for-Sexual-Medicine has highlighted the limitations of using the rat model for nerve-sparing prostatectomy. The use of young rats with no comorbidities and the early evaluation of the erectile function (EF) are deemed a source of bias. Our aim was to evaluate the long-term consequences in EF of bilateral nerve cavernous crush- injury (BNCI) in type 1 diabetic (DM) rats 30-male/12-week-old rats were divided into four groups: Sham, BNCI, DM, and BNCI + DM. Sham group underwent an intraperitoneal injection (IP) of saline solution and after 1 month underwent a sham laparotomy. BNCI underwent an IP of saline solution and after 1 month to BNCI. DM underwent an IP of 60 mg/kg-1-streptozotocin (STZ)... (More)

A recent statement from the European-Society-for-Sexual-Medicine has highlighted the limitations of using the rat model for nerve-sparing prostatectomy. The use of young rats with no comorbidities and the early evaluation of the erectile function (EF) are deemed a source of bias. Our aim was to evaluate the long-term consequences in EF of bilateral nerve cavernous crush- injury (BNCI) in type 1 diabetic (DM) rats 30-male/12-week-old rats were divided into four groups: Sham, BNCI, DM, and BNCI + DM. Sham group underwent an intraperitoneal injection (IP) of saline solution and after 1 month underwent a sham laparotomy. BNCI underwent an IP of saline solution and after 1 month to BNCI. DM underwent an IP of 60 mg/kg-1-streptozotocin (STZ) and after 1 month to a sham laparotomy. BNCI + DM underwent an IP of 60 mg/kg-1-STZ and after 1 month to BNCI. After 5 months from the induction of diabetes, all rats underwent measurement of intracorporeal pressure (ICP) and mean arterial pressure (MAP) during CN-electrostimulation. Multiple groups were compared using Kruskal–Wallis one-way analysis of variance followed by Mann–Whitney U test for post hoc comparisons. Blood glucose-level was higher (p < 0.05) in the groups with DM and BNCI + DM. After 5-months, DM and BNCI + DM also showed a lower weight compared to other groups (p < 0.05). No differences were noted in ICP/MAP between the sham and BNCI. BNCI + DM showed lower ICP/MAP compared to all the groups (p < 0.05). DM Showed lower ICP/MAP compared to Sham and BNCI (p < 0.05). BNCI in rats without comorbidities did not induce long-term erectile dysfunction (ED) suggesting a spontaneous EF recovery. BNCI in DM induced long-term ED. The results of previous short-term studies can only provide evidence on the time to recovery of spontaneous EF as to the actual EF recovery rate.

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type
Contribution to journal
publication status
published
subject
in
International Journal of Impotence Research
volume
34
issue
8
pages
781 - 785
publisher
Nature Publishing Group
external identifiers
  • scopus:85116827636
  • pmid:34611324
ISSN
0955-9930
DOI
10.1038/s41443-021-00474-z
language
English
LU publication?
yes
additional info
Publisher Copyright: © 2021, The Author(s), under exclusive licence to Springer Nature Limited.
id
764d461f-cc1d-49c5-9094-ad1b29489681
date added to LUP
2021-11-16 17:49:18
date last changed
2024-06-15 20:30:17
@article{764d461f-cc1d-49c5-9094-ad1b29489681,
  abstract     = {{<p>A recent statement from the European-Society-for-Sexual-Medicine has highlighted the limitations of using the rat model for nerve-sparing prostatectomy. The use of young rats with no comorbidities and the early evaluation of the erectile function (EF) are deemed a source of bias. Our aim was to evaluate the long-term consequences in EF of bilateral nerve cavernous crush- injury (BNCI) in type 1 diabetic (DM) rats 30-male/12-week-old rats were divided into four groups: Sham, BNCI, DM, and BNCI + DM. Sham group underwent an intraperitoneal injection (IP) of saline solution and after 1 month underwent a sham laparotomy. BNCI underwent an IP of saline solution and after 1 month to BNCI. DM underwent an IP of 60 mg/kg-1-streptozotocin (STZ) and after 1 month to a sham laparotomy. BNCI + DM underwent an IP of 60 mg/kg-1-STZ and after 1 month to BNCI. After 5 months from the induction of diabetes, all rats underwent measurement of intracorporeal pressure (ICP) and mean arterial pressure (MAP) during CN-electrostimulation. Multiple groups were compared using Kruskal–Wallis one-way analysis of variance followed by Mann–Whitney U test for post hoc comparisons. Blood glucose-level was higher (p &lt; 0.05) in the groups with DM and BNCI + DM. After 5-months, DM and BNCI + DM also showed a lower weight compared to other groups (p &lt; 0.05). No differences were noted in ICP/MAP between the sham and BNCI. BNCI + DM showed lower ICP/MAP compared to all the groups (p &lt; 0.05). DM Showed lower ICP/MAP compared to Sham and BNCI (p &lt; 0.05). BNCI in rats without comorbidities did not induce long-term erectile dysfunction (ED) suggesting a spontaneous EF recovery. BNCI in DM induced long-term ED. The results of previous short-term studies can only provide evidence on the time to recovery of spontaneous EF as to the actual EF recovery rate.</p>}},
  author       = {{Castiglione, Fabio and Albersen, Maarten and Fiorenzo, Salvatore and Hedlund, Petter and Cakir, Omer Onur and Pavone, Carlo and Alnajjar, Hussain M. and Joniau, Steven and Muneer, Asif and Kluth, Luis A. and Campos-Juanatey, Felix and Cocci, Andrea and Rosenbaum, Clemens M. and Vetterlein, Malte W. and Fes, Enrique and Adamowicz, Jan}},
  issn         = {{0955-9930}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{781--785}},
  publisher    = {{Nature Publishing Group}},
  series       = {{International Journal of Impotence Research}},
  title        = {{Long-term consequences of bilateral cavernous crush injury in normal and diabetic rats : a functional study}},
  url          = {{http://dx.doi.org/10.1038/s41443-021-00474-z}},
  doi          = {{10.1038/s41443-021-00474-z}},
  volume       = {{34}},
  year         = {{2022}},
}