Fluorometric broad-range screening of compounds with affinity for nucleic acids
(2005) In Analytical Chemistry 77(8). p.2450-2454- Abstract
- The potential of a nucleic acid-based optical bioprobe for environmental measurements and drug monitoring is described. The sensor employs the long-wavelength intercalating fluorophore TO-PRO-3 (TP3). Compounds that interact with the TP3-DNA complex are indirectly detected by a decrease in the fluorescence intensity. We found that the configuration and length of the DNA dramatically affected the intensity of the fluorescence emitted from the TP3-DNA complex. We compared nucleic acids from different sources and optimized the system for pBR322 plasmid DNA (4363 bp) digested by HindIII restriction endonuclease. This endonuclease has a single recognition site in plasmid pBR322. In the proposed method, we attempt to combine broad-range... (More)
- The potential of a nucleic acid-based optical bioprobe for environmental measurements and drug monitoring is described. The sensor employs the long-wavelength intercalating fluorophore TO-PRO-3 (TP3). Compounds that interact with the TP3-DNA complex are indirectly detected by a decrease in the fluorescence intensity. We found that the configuration and length of the DNA dramatically affected the intensity of the fluorescence emitted from the TP3-DNA complex. We compared nucleic acids from different sources and optimized the system for pBR322 plasmid DNA (4363 bp) digested by HindIII restriction endonuclease. This endonuclease has a single recognition site in plasmid pBR322. In the proposed method, we attempt to combine broad-range detection with rapid and simple operation. A fiber-optic capillary fluorescence system was used to analyze toxic aromatic amines, antibiotics, and several kinds of antitumor drugs, using small amounts of sample, down to 10 mu L, with a sensitivity comparable to that of current electrochemical methods. The detection limit can be as low as a few ppb or submicromolar. This approach is useful for routine screening in environmental monitoring or for controlling cytotoxic drug administration. The ease of operation and the rapid response allow high-throughput screening. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/152647
- author
- Liu, Yang LU and Danielsson, Bengt LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical Chemistry
- volume
- 77
- issue
- 8
- pages
- 2450 - 2454
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000228605100023
- scopus:17644418461
- ISSN
- 1520-6882
- DOI
- 10.1021/ac048449o
- language
- English
- LU publication?
- yes
- id
- 76601810-4731-490b-aa45-80546900e9b9 (old id 152647)
- date added to LUP
- 2016-04-01 11:51:19
- date last changed
- 2022-01-26 19:14:48
@article{76601810-4731-490b-aa45-80546900e9b9, abstract = {{The potential of a nucleic acid-based optical bioprobe for environmental measurements and drug monitoring is described. The sensor employs the long-wavelength intercalating fluorophore TO-PRO-3 (TP3). Compounds that interact with the TP3-DNA complex are indirectly detected by a decrease in the fluorescence intensity. We found that the configuration and length of the DNA dramatically affected the intensity of the fluorescence emitted from the TP3-DNA complex. We compared nucleic acids from different sources and optimized the system for pBR322 plasmid DNA (4363 bp) digested by HindIII restriction endonuclease. This endonuclease has a single recognition site in plasmid pBR322. In the proposed method, we attempt to combine broad-range detection with rapid and simple operation. A fiber-optic capillary fluorescence system was used to analyze toxic aromatic amines, antibiotics, and several kinds of antitumor drugs, using small amounts of sample, down to 10 mu L, with a sensitivity comparable to that of current electrochemical methods. The detection limit can be as low as a few ppb or submicromolar. This approach is useful for routine screening in environmental monitoring or for controlling cytotoxic drug administration. The ease of operation and the rapid response allow high-throughput screening.}}, author = {{Liu, Yang and Danielsson, Bengt}}, issn = {{1520-6882}}, language = {{eng}}, number = {{8}}, pages = {{2450--2454}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Analytical Chemistry}}, title = {{Fluorometric broad-range screening of compounds with affinity for nucleic acids}}, url = {{http://dx.doi.org/10.1021/ac048449o}}, doi = {{10.1021/ac048449o}}, volume = {{77}}, year = {{2005}}, }