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Extracellular Electron Transfer by the Gram-positive Bacterium Enterococcus faecalis

Pankratova, Galina LU ; Leech, Dónal; Gorton, Lo LU and Hederstedt, Lars LU (2018) In Biochemistry 57. p.4597-4603
Abstract
Extracellular electron transfer (EET) in microbial cells is essential for certain biotechnological applications and contributes to the biogeochemical cycling of elements and syntrophic microbial metabolism in complex natural environments. The Gram-positive lactic acid bacterium Enterococcus faecalis, an opportunistic human pathogen, is shown to be able to transfer electrons generated in fermentation metabolism to electrodes directly and indirectly via mediators. By exploiting E. faecalis wild-type and mutant cells it is demonstrated that reduced demethylmenaquinone in the respiratory chain in the bacterial cytoplasmic membrane is crucial for the EET. Heme-proteins are not involved and cytochrome bd oxidase activity was found to attenuate... (More)
Extracellular electron transfer (EET) in microbial cells is essential for certain biotechnological applications and contributes to the biogeochemical cycling of elements and syntrophic microbial metabolism in complex natural environments. The Gram-positive lactic acid bacterium Enterococcus faecalis, an opportunistic human pathogen, is shown to be able to transfer electrons generated in fermentation metabolism to electrodes directly and indirectly via mediators. By exploiting E. faecalis wild-type and mutant cells it is demonstrated that reduced demethylmenaquinone in the respiratory chain in the bacterial cytoplasmic membrane is crucial for the EET. Heme-proteins are not involved and cytochrome bd oxidase activity was found to attenuate EET. These results are significant for the mechanistic understanding of EET in bacteria and for design of microbial electrochemical systems. The basic findings infer that in dense microbial communities, such as in biofilm and in the large intestine, metabolism in E. faecalis and similar Gram-positive lactic acid bacteria might be electrically connected to other microbes. Such an intercellular electron transfer might confer syntrophic metabolism that promote growth and other activities of bacteria in the microbiota of humans and animals. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biochemistry
volume
57
pages
4597 - 4603
publisher
The American Chemical Society
external identifiers
  • scopus:85049829447
ISSN
0006-2960
DOI
10.1021/acs.biochem.8b00600
language
English
LU publication?
yes
id
76b14085-9dcc-4966-8c04-78767a9db48f
date added to LUP
2018-07-12 08:50:47
date last changed
2018-10-03 10:52:16
@article{76b14085-9dcc-4966-8c04-78767a9db48f,
  abstract     = {Extracellular electron transfer (EET) in microbial cells is essential for certain biotechnological applications and contributes to the biogeochemical cycling of elements and syntrophic microbial metabolism in complex natural environments. The Gram-positive lactic acid bacterium Enterococcus faecalis, an opportunistic human pathogen, is shown to be able to transfer electrons generated in fermentation metabolism to electrodes directly and indirectly via mediators. By exploiting E. faecalis wild-type and mutant cells it is demonstrated that reduced demethylmenaquinone in the respiratory chain in the bacterial cytoplasmic membrane is crucial for the EET. Heme-proteins are not involved and cytochrome bd oxidase activity was found to attenuate EET. These results are significant for the mechanistic understanding of EET in bacteria and for design of microbial electrochemical systems. The basic findings infer that in dense microbial communities, such as in biofilm and in the large intestine, metabolism in E. faecalis and similar Gram-positive lactic acid bacteria might be electrically connected to other microbes. Such an intercellular electron transfer might confer syntrophic metabolism that promote growth and other activities of bacteria in the microbiota of humans and animals.},
  author       = {Pankratova, Galina and Leech, Dónal and Gorton, Lo and Hederstedt, Lars},
  issn         = {0006-2960},
  language     = {eng},
  pages        = {4597--4603},
  publisher    = {The American Chemical Society},
  series       = {Biochemistry},
  title        = {Extracellular Electron Transfer by the Gram-positive Bacterium Enterococcus faecalis},
  url          = {http://dx.doi.org/10.1021/acs.biochem.8b00600},
  volume       = {57},
  year         = {2018},
}